##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AscI        Asp718I     AspI        AviII       BbrPI       
BcgI        BfrI        BsaAI       BsaBI       BseAI       
BsiWI       BspDI       BspEI       BsrDI       BstBI       
ClaI        DrdI        EcoNI       EcoRV       EcoT22I     
FspI        HindIII     KpnI        MamI        MfeI        
MluI        MroI        MunI        NheI        NotI        
NruI        NsiI        NspV        PacI        PflFI       
PmaCI       PmeI        PmlI        PshAI       Psp1406I    
PvuI        SalI        SapI        SbfI        ScaI        
SfiI        SfuI        SnaBI       SpeI        SphI        
Sse8387I    SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AccI         5647  GTATAC               GT^[AC][GT]AC
AgeI         2732  ACCGGT               A^CCGGT
AhdI         1101  GACCCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        1101  GACCCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI        5101  GGATCC               G^GATCC
BciVI        4122  GTATCC               GTATCC(6/5)
BclI          943  TGATCA               T^GATCA
BglII        4878  AGATCT               A^GATCT
BlpI         2944  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2944  GCTCAGC              GC^T[ACGT]AGC
BsaWI        2732  ACCGGT               [AT]^CCGG[AT]
Bsp1407I     4813  TGTACA               T^GTACA
BsrGI        4813  TGTACA               T^GTACA
BssSI        1917  CACGAG               CACGAG(-5/-1)
Bst1107I     5647  GTATAC               GTA^TAC
BstEII       5053  GGTGACC              G^GT[ACGT]ACC
BstPI        5053  GGTGACC              G^GT[ACGT]ACC
BstZ17I      5647  GTATAC               GTA^TAC
CelII        2944  GCTCAGC              GC^T[ACGT]AGC
CpoI         3153  CGGACCG              CG^G[AT]CCG
DraIII       4602  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     1101  GACCCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      5053  GGTGACC              G^GT[ACGT]ACC
FbaI          943  TGATCA               T^GATCA
FseI         4372  GGCCGGCC             GGCCGG^CC
HpaI         5572  GTTAAC               GTT^AAC
NdeI         4093  CATATG               CA^TATG
PaeR7I       2389  CTCGAG               C^TCGAG
PinAI        2732  ACCGGT               A^CCGGT
RsrII        3153  CGGACCG              CG^G[AT]CCG
SgrAI         333  CGCCGGCG             C[GA]^CCGG[CT]G
SspBI        4813  TGTACA               T^GTACA
SspI         1152  AATATT               AAT^ATT
XhoI         2389  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AflIII       4168  ACATGT               A^C[GA][CT]GT
             4533  ACATGT              
Aor51HI       445  AGCGCT               AGC^GCT
             5458  AGCGCT              
AseI         1149  ATTAAT               AT^TAAT
             5611  ATTAAT              
AsnI         1149  ATTAAT               AT^TAAT
             5611  ATTAAT              
Asp700I      4051  GAATCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4385  GAAGCAATTC          
AvrII        1283  CCTAGG               C^CTAGG
             3843  CCTAGG              
BbeI          355  GGCGCC               GGCGC^C
              512  GGCGCC              
BlnI         1283  CCTAGG               C^CTAGG
             3843  CCTAGG              
BsaI         4610  GAGACC               GGTCTC(1/5)
             3304  GGTCTC              
BsmBI        2639  CGTCTC               CGTCTC(1/5)
             5141  CGTCTC              
BsmI         2791  GAATGC               GAATGC(1/-1)
             4645  GAATGC              
BspHI         584  TCATGA               T^CATGA
             1657  TCATGA              
BspLU11I     4168  ACATGT               A^CATGT
             4533  ACATGT              
BsrBI         134  CCGCTC               CCGCTC(-3/-3)
               96  GAGCGG              
BssHII        124  GCGCGC               G^CGCGC
              470  GCGCGC              
BstAPI       2132  GCATCAGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3038  GCAGGGCTTGC         
BstXI        1307  CCACACCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3503  CCATCGGGCTGG        
BstYI        4878  AGATCT               [GA]^GATC[CT]
             5101  GGATCC              
Eco47III      445  AGCGCT               AGC^GCT
             5458  AGCGCT              
HincII       3945  GTTGAC               GT[CT]^[GA]AC
             5572  GTTAAC              
HindII       3945  GTTGAC               GT[CT]^[GA]AC
             5572  GTTAAC              
KasI          355  GGCGCC               G^GCGCC
              512  GGCGCC              
MaeII        2620  ACGT                 A^CGT
             2830  ACGT                
MflI         4878  AGATCT               [GA]^GATC[CT]
             5101  GGATCC              
NarI          355  GGCGCC               GG^CGCC
              512  GGCGCC              
PshBI        1149  ATTAAT               AT^TAAT
             5611  ATTAAT              
RcaI          584  TCATGA               T^CATGA
             1657  TCATGA              
SacI         1020  GAGCTC               GAGCT^C
             2942  GAGCTC              
SexAI        3540  ACCTGGT              A^CC[AT]GGT
             5299  ACCTGGT             
SfoI          355  GGCGCC               GGC^GCC
              512  GGCGCC              
SmlI           58  CTTGAG               C^T[CT][GA]AG
             2389  CTCGAG              
StuI         3139  AGGCCT               AGG^CCT
             3448  AGGCCT              
XhoII        4878  AGATCT               [GA]^GATC[CT]
             5101  GGATCC              
XmnI         4051  GAATCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4385  GAAGCAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  131 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AluI        Alw44I      AlwI        AlwNI       AocI        
ApaI        ApaLI       ApoI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BpuAI       BsaHI       BsaJI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       Bsu36I      Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EaeI        EagI        EarI        
EclXI       Eco52I      Eco81I      EcoO109I    EcoRI       
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     KspI        MaeI        MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PflMI       PleI        PpuMI       PstI        PvuII       
RsaI        SacII       Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XmaCI       
XmaI