##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI Asp718I AspEI AspHI AviII
AvrII BanII BbeI BbrPI BbsI
BcgI BfrI BglI BlnI BpuAI
BsaBI BsaHI BsaWI BseAI BsiHKAI
BsiWI BsmBI BspDI BspEI BssSI
Bst1107I BstEII BstPI BstZ17I ClaI
DraIII DrdI EagI Eam1105I EclXI
Eco52I EcoNI EcoO65I EcoT22I FseI
FspI HgaI Hin1I HpaI HphI
KasI KpnI KspI MamI MluI
MroI NarI NdeI NheI NotI
NruI NsiI PacI PinAI PmaCI
PmeI PmlI PshAI PvuI SacI
SacII SapI SbfI SfiI SfoI
SgrAI SmaI SnaBI Sse8387I StuI
SwaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AclI 2727 AACGTT AA^CGTT
AocI 318 CCTAAGG CC^T[ACGT]AGG
Aor51HI 3374 AGCGCT AGC^GCT
AseI 2071 ATTAAT AT^TAAT
AsnI 2071 ATTAAT AT^TAAT
AspI 2551 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 2253 GCTGAGG CCTCAGC(-5/-2)
BmyI 3644 GTGCCC G[AGT]GC[ACT]^C
BsaAI 1048 TACGTG [CT]AC^GT[GA]
BsaI 984 GGTCTC GGTCTC(1/5)
BsiEI 3473 CGACCG CG[GA][CT]^CG
Bsp1286I 3644 GTGCCC G[AGT]GC[ACT]^C
BspHI 3008 TCATGA T^CATGA
BspMI 2093 ACCTGC ACCTGC(4/8)
BsrFI 90 GCCGGC [GA]^CCGG[CT]
BssHII 99 GCGCGC G^CGCGC
BstAPI 1201 GCAAAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 842 TTCGAA TT^CGAA
Bsu36I 318 CCTAAGG CC^T[ACGT]AGG
Cfr10I 90 GCCGGC [GA]^CCGG[CT]
CpoI 260 CGGTCCG CG^G[AT]CCG
Eco47III 3374 AGCGCT AGC^GCT
Eco81I 318 CCTAAGG CC^T[ACGT]AGG
EcoRI 1801 GAATTC G^AATTC
MfeI 3392 CAATTG C^AATTG
MunI 3392 CAATTG C^AATTG
NaeI 90 GCCGGC GCC^GGC
NgoMIV 90 GCCGGC G^CCGGC
NspV 842 TTCGAA TT^CGAA
PflFI 2551 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 2071 ATTAAT AT^TAAT
Psp1406I 2727 AACGTT AA^CGTT
RcaI 3008 TCATGA T^CATGA
RsrII 260 CGGTCCG CG^G[AT]CCG
ScaI 2111 AGTACT AGT^ACT
SfuI 842 TTCGAA TT^CGAA
SpeI 4098 ACTAGT A^CTAGT
SphI 1284 GCATGC GCATG^C
SspI 1719 AATATT AAT^ATT
Tth111I 2551 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 609 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflIII 2782 ACATGT A^C[GA][CT]GT
3708 ACATGT
BamHI 1496 GGATCC G^GATCC
2373 GGATCC
BsmI 3012 GAATGC GAATGC(1/-1)
3335 GCATTC
BspLU11I 2782 ACATGT A^CATGT
3708 ACATGT
BsrBI 24 CCGCTC CCGCTC(-3/-3)
1615 GAGCGG
BstXI 463 CCAGAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1126 CCAGATAATTGG
DraII 1928 GGGACCT [GA]G^G[ACGT]CC[CT]
3260 AGGACCC
EcoO109I 1928 GGGACCT [GA]G^G[ACGT]CC[CT]
3260 AGGACCC
EcoRV 430 GATATC GAT^ATC
605 GATATC
HaeII 3374 AGCGCT [GA]GCGC^[CT]
3561 GGCGCT
NcoI 2003 CCATGG C^CATGG
2513 CCATGG
PaeR7I 2462 CTCGAG C^TCGAG
3106 CTCGAG
PflMI 1388 CCATCCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1806 CCACCAGTTGG
PpuMI 1928 GGGACCT [GA]G^G[AT]CC[CT]
3260 AGGACCC
PstI 2745 CTGCAG CTGCA^G
3629 CTGCAG
SalI 953 GTCGAC G^TCGAC
2738 GTCGAC
Van91I 1388 CCATCCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1806 CCACCAGTTGG
XhoI 2462 CTCGAG C^TCGAG
3106 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
AvaI AvaII BalI BanI BbvI
BciVI BclI BcnI BfaI BglII
BlpI BpmI Bpu1102I BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1407I BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DsaI EaeI EarI
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI