##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BbeI
BbrPI BfrI BlnI BsaAI BsaWI
BseAI BsiWI BspDI BspEI BspLU11I
BssHII Bst1107I BstAPI BstBI BstZ17I
ClaI CpoI DraI Eco47III EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI KasI KpnI KspI MroI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AflIII 4701 ACGCGT A^C[GA][CT]GT
AhdI 718 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 718 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 2494 GCAAGTGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2257 TGATCA T^GATCA
BglII 2665 AGATCT A^GATCT
BsaBI 3161 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3910 CGGCCG CG[GA][CT]^CG
BsmI 494 GAATGC GAATGC(1/-1)
Bsp1407I 129 TGTACA T^GTACA
BspHI 4353 TCATGA T^CATGA
BsrGI 129 TGTACA T^GTACA
BssSI 4632 CTCGTG CACGAG(-5/-1)
EagI 3910 CGGCCG C^GGCCG
Eam1105I 718 GACACCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3910 CGGCCG C^GGCCG
Eco52I 3910 CGGCCG C^GGCCG
FbaI 2257 TGATCA T^GATCA
MamI 3161 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 5015 CAATTG C^AATTG
MluI 4701 ACGCGT A^CGCGT
MunI 5015 CAATTG C^AATTG
NaeI 1071 GCCGGC GCC^GGC
NgoMIV 1071 GCCGGC G^CCGGC
PflMI 3997 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3886 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4353 TCATGA T^CATGA
ScaI 190 AGTACT AGT^ACT
SexAI 721 ACCTGGT A^CC[AT]GGT
SspBI 129 TGTACA T^GTACA
Tsp509I 5016 AATT ^AATT
Van91I 3997 CCAACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 2757 GTCTAC GT^[AC][GT]AC
4989 GTAGAC
AcyI 1164 GGCGTC G[GA]^CG[CT]C
1602 GACGCC
BfaI 3114 CTAG C^TAG
4542 CTAG
BsaHI 1164 GGCGTC G[GA]^CG[CT]C
1602 GACGCC
BsmBI 712 GAGACG CGTCTC(1/5)
1600 GAGACG
BsrDI 2036 CATTGC GCAATG(2/0)
3257 CATTGC
DraIII 2540 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
3069 CACCAAGTG
DrdI 4086 GACAGCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4415 GACCCCGCTGTC
Hin1I 1164 GGCGTC G[GA]^CG[CT]C
1602 GACGCC
HincII 726 GTCAAC GT[CT]^[GA]AC
2736 GTCAAC
HindII 726 GTCAAC GT[CT]^[GA]AC
2736 GTCAAC
MaeI 3114 CTAG C^TAG
4542 CTAG
MseI 2272 TTAA T^TAA
5004 TTAA
NcoI 202 CCATGG C^CATGG
2309 CCATGG
PpuMI 1543 AGGACCT [GA]G^G[AT]CC[CT]
4637 GGGTCCC
SacI 2600 GAGCTC GAGCT^C
2778 GAGCTC
SapI 1785 GAAGAGC GCTCTTC(1/4)
4487 GCTCTTC
TaqI 2503 TCGA T^CGA
3439 TCGA
Tru9I 2272 TTAA T^TAA
5004 TTAA
TthHB8I 2503 TCGA T^CGA
3439 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbsI BbvCI BbvI BciVI BcnI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SphI StuI StyI TfiI
TseI Tsp45I TspRI XcmI XhoII
XmaCI XmaI