##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AclI AgeI Alw44I
ApaLI AscI AseI AsnI Asp700I
Asp718I AviII BsaBI BsiWI BsmBI
BspDI BspHI BspLU11I BsrFI BssHII
BssSI BstEII BstPI Cfr10I ClaI
CpoI DraI DraIII EcoO65I EcoRV
EcoT22I FseI FspI HincII HindII
HindIII HpaI KpnI MamI MfeI
MluI MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SfiI SgrAI SmaI SnaBI SpeI
SwaI Van91I XbaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 2383 GTATAC GT^[AC][GT]AC
AccIII 1956 TCCGGA T^CCGGA
AcyI 2839 GGCGCC G[GA]^CG[CT]C
AflII 3043 CTTAAG C^TTAAG
AflIII 560 ACGTGT A^C[GA][CT]GT
AhdI 2333 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3022 AGCGCT AGC^GCT
AspEI 2333 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2328 CCTAGG C^CTAGG
BalI 2626 TGGCCA TGG^CCA
BamHI 1953 GGATCC G^GATCC
BbeI 2839 GGCGCC GGCGC^C
BbvCI 1471 CCTCAGC CCTCAGC(-5/-2)
BciVI 515 GGATAC GTATCC(6/5)
BfrI 3043 CTTAAG C^TTAAG
BglII 1050 AGATCT A^GATCT
BlnI 2328 CCTAGG C^CTAGG
BsaHI 2839 GGCGCC G[GA]^CG[CT]C
BsaI 2029 GGTCTC GGTCTC(1/5)
BsaWI 1956 TCCGGA [AT]^CCGG[AT]
BseAI 1956 TCCGGA T^CCGGA
BsiEI 138 CGGCCG CG[GA][CT]^CG
Bsp1407I 563 TGTACA T^GTACA
BspEI 1956 TCCGGA T^CCGGA
BsrBI 193 GAGCGG CCGCTC(-3/-3)
BsrGI 563 TGTACA T^GTACA
Bst1107I 2383 GTATAC GTA^TAC
BstBI 634 TTCGAA TT^CGAA
BstXI 3028 CCAGGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2383 GTATAC GTA^TAC
DrdI 3528 GACGAGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 138 CGGCCG C^GGCCG
Eam1105I 2333 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 138 CGGCCG C^GGCCG
Eco47III 3022 AGCGCT AGC^GCT
Eco52I 138 CGGCCG C^GGCCG
EcoRI 691 GAATTC G^AATTC
Hin1I 2839 GGCGCC G[GA]^CG[CT]C
KasI 2839 GGCGCC G^GCGCC
MluNI 2626 TGGCCA TGG^CCA
MroI 1956 TCCGGA T^CCGGA
MscI 2626 TGGCCA TGG^CCA
NarI 2839 GGCGCC GG^CGCC
NspV 634 TTCGAA TT^CGAA
PaeR7I 2161 CTCGAG C^TCGAG
SapI 589 GAAGAGC GCTCTTC(1/4)
SbfI 963 CCTGCAGG CCTGCA^GG
ScaI 3208 AGTACT AGT^ACT
SexAI 2783 ACCAGGT A^CC[AT]GGT
SfoI 2839 GGCGCC GGC^GCC
SfuI 634 TTCGAA TT^CGAA
SphI 2732 GCATGC GCATG^C
Sse8387I 963 CCTGCAGG CCTGCA^GG
SspBI 563 TGTACA T^GTACA
SspI 1790 AATATT AAT^ATT
XcmI 2228 CCAAGGTACGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2161 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 631 AAATTC [GA]^AATT[CT]
691 GAATTC
AlwNI 1062 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
3340 CAGAATCTG
AocI 272 CCTTAGG CC^T[ACGT]AGG
2833 CCTGAGG
ApaI 94 GGGCCC GGGCC^C
3373 GGGCCC
ApoI 631 AAATTC [GA]^AATT[CT]
691 GAATTC
AspI 1614 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2068 GACCCTGTC
BbsI 976 GAAGAC GAAGAC(2/6)
1620 GTCTTC
BlpI 1031 GCTTAGC GC^T[ACGT]AGC
2035 GCTGAGC
Bpu1102I 1031 GCTTAGC GC^T[ACGT]AGC
2035 GCTGAGC
BpuAI 976 GAAGAC GAAGAC(2/6)
1620 GTCTTC
BsmI 2049 GAATGC GAATGC(1/-1)
1352 GCATTC
Bsu36I 272 CCTTAGG CC^T[ACGT]AGG
2833 CCTGAGG
CelII 1031 GCTTAGC GC^T[ACGT]AGC
2035 GCTGAGC
EaeI 138 CGGCCG [CT]^GGCC[GA]
2626 TGGCCA
Eco81I 272 CCTTAGG CC^T[ACGT]AGG
2833 CCTGAGG
EcoNI 2623 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2954 CCTCTCGGAGG
KspI 90 CCGCGG CCGC^GG
129 CCGCGG
NcoI 1191 CCATGG C^CATGG
3357 CCATGG
PflFI 1614 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2068 GACCCTGTC
SacI 592 GAGCTC GAGCT^C
2755 GAGCTC
SacII 90 CCGCGG CCGC^GG
129 CCGCGG
StuI 1121 AGGCCT AGG^CCT
2799 AGGCCT
Tth111I 1614 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2068 GACCCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbrPI BbvI BcgI BclI BcnI
BfaI BglI BmyI BpmI BsaAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII