##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AflII AgeI Aor51HI AscI AseI
AsnI AspI AvrII BbrPI BcgI
BciVI BclI BfrI BlnI BsaAI
BsaBI BsiEI BsiWI BsmI Bsp1407I
BspDI BspLU11I BsrDI BsrGI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DraIII EagI EclXI Eco47III Eco52I
EcoRI EcoRV FbaI FseI HindIII
HpaI KspI MamI MfeI MunI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI PvuI RsrII
SacI SacII SapI ScaI SexAI
SfuI SgrAI SmlI SnaBI SpeI
SspBI SspI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 1834 GGTACC G^GTACC
AccIII 736 TCCGGA T^CCGGA
AclI 121 AACGTT AA^CGTT
AflIII 3245 ACGCGT A^C[GA][CT]GT
Asp718I 1834 GGTACC G^GTACC
AviII 1162 TGCGCA TGC^GCA
BglI 1123 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1633 AGATCT A^GATCT
BlpI 2142 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2142 GCTGAGC GC^T[ACGT]AGC
BsaI 2381 GGTCTC GGTCTC(1/5)
BsaWI 736 TCCGGA [AT]^CCGG[AT]
BseAI 736 TCCGGA T^CCGGA
BsmBI 720 GAGACG CGTCTC(1/5)
BspEI 736 TCCGGA T^CCGGA
BspHI 1525 TCATGA T^CATGA
BsrBI 302 GAGCGG CCGCTC(-3/-3)
BssSI 156 CACGAG CACGAG(-5/-1)
BstAPI 389 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 695 GGTGACC G^GT[ACGT]ACC
BstPI 695 GGTGACC G^GT[ACGT]ACC
CelII 2142 GCTGAGC GC^T[ACGT]AGC
DrdI 1266 GACACCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 695 GGTGACC G^GT[ACGT]ACC
EcoT22I 2394 ATGCAT ATGCA^T
FspI 1162 TGCGCA TGC^GCA
HincII 1731 GTCGAC GT[CT]^[GA]AC
HindII 1731 GTCGAC GT[CT]^[GA]AC
KpnI 1834 GGTACC GGTAC^C
MluI 3245 ACGCGT A^CGCGT
MroI 736 TCCGGA T^CCGGA
NaeI 10 GCCGGC GCC^GGC
NcoI 524 CCATGG C^CATGG
NgoMIV 10 GCCGGC G^CCGGC
NsiI 2394 ATGCAT ATGCA^T
PflMI 3111 CCAGTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1616 GACGGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 121 AACGTT AA^CGTT
RcaI 1525 TCATGA T^CATGA
SalI 1731 GTCGAC G^TCGAC
SbfI 1828 CCTGCAGG CCTGCA^GG
SfiI 1122 GGCCCCGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 745 GCATGC GCATG^C
Sse8387I 1828 CCTGCAGG CCTGCA^GG
Van91I 3111 CCAGTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AatII 1620 GACGTC GACGT^C
1857 GACGTC
AhdI 2297 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3346 GACATGCAGTC
AspEI 2297 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3346 GACATGCAGTC
BalI 418 TGGCCA TGG^CCA
925 TGGCCA
BstXI 421 CCAACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1883 CCAGCCCGGTGG
Eam1105I 2297 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3346 GACATGCAGTC
MluNI 418 TGGCCA TGG^CCA
925 TGGCCA
MscI 418 TGGCCA TGG^CCA
925 TGGCCA
PleI 2197 GAGTC GAGTC(4/5)
3004 GAGTC
StuI 61 AGGCCT AGG^CCT
2039 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI Asp700I
AspHI AvaI AvaII BamHI BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV NspI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI XmnI