##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AclI AflII AscI AviII AvrII
BbeI BclI BfrI BglII BlnI
BsaBI BsiWI BsmI BspDI BspLU11I
BssHII BssSI BstAPI BstBI ClaI
CpoI DraI DrdI EcoRV EcoT22I
FbaI FseI FspI HpaI KasI
MamI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PmeI Psp1406I PvuI RsrII SalI
SbfI SfiI SfoI SfuI SnaBI
SpeI SphI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 1292 GACGTC GACGT^C
Acc65I 980 GGTACC G^GTACC
AccI 2784 GTATAC GT^[AC][GT]AC
AccIII 1425 TCCGGA T^CCGGA
AcyI 1292 GACGTC G[GA]^CG[CT]C
AgeI 1040 ACCGGT A^CCGGT
AhdI 2321 GACCAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 250 AGCGCT AGC^GCT
AseI 1379 ATTAAT AT^TAAT
AsnI 1379 ATTAAT AT^TAAT
Asp718I 980 GGTACC G^GTACC
AspEI 2321 GACCAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1532 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2635 CACGTG CAC^GTG
BciVI 3434 GGATAC GTATCC(6/5)
BsaHI 1292 GACGTC G[GA]^CG[CT]C
BseAI 1425 TCCGGA T^CCGGA
BspEI 1425 TCCGGA T^CCGGA
BsrDI 1299 GCAATG GCAATG(2/0)
Bst1107I 2784 GTATAC GTA^TAC
BstEII 2674 GGTCACC G^GT[ACGT]ACC
BstPI 2674 GGTCACC G^GT[ACGT]ACC
BstZ17I 2784 GTATAC GTA^TAC
DraIII 1403 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 3531 CGGCCG C^GGCCG
Eam1105I 2321 GACCAAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3531 CGGCCG C^GGCCG
Eco47III 250 AGCGCT AGC^GCT
Eco52I 3531 CGGCCG C^GGCCG
EcoO65I 2674 GGTCACC G^GT[ACGT]ACC
HgaI 3798 GACGC GACGC(5/10)
Hin1I 1292 GACGTC G[GA]^CG[CT]C
HincII 2477 GTTGAC GT[CT]^[GA]AC
HindII 2477 GTTGAC GT[CT]^[GA]AC
KpnI 980 GGTACC GGTAC^C
KspI 365 CCGCGG CCGC^GG
MroI 1425 TCCGGA T^CCGGA
NcoI 63 CCATGG C^CATGG
NspI 1752 GCATGT [GA]CATG^[CT]
PflFI 1532 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1040 ACCGGT A^CCGGT
PmaCI 2635 CACGTG CAC^GTG
PmlI 2635 CACGTG CAC^GTG
PshAI 781 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1379 ATTAAT AT^TAAT
SacII 365 CCGCGG CCGC^GG
SexAI 3738 ACCTGGT A^CC[AT]GGT
SgrAI 1039 CACCGGTG C[GA]^CCGG[CT]G
SmaI 4028 CCCGGG CCC^GGG
SspI 305 AATATT AAT^ATT
StuI 1966 AGGCCT AGG^CCT
Tth111I 1532 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1990 TCTAGA T^CTAGA
XmaCI 4028 CCCGGG C^CCGGG
XmaI 4028 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 2272 GAATTC [GA]^AATT[CT]
4247 GAATTC
Alw44I 1559 GTGCAC G^TGCAC
3591 GTGCAC
AocI 408 CCTCAGG CC^T[ACGT]AGG
2627 CCTGAGG
ApaLI 1559 GTGCAC G^TGCAC
3591 GTGCAC
ApoI 2272 GAATTC [GA]^AATT[CT]
4247 GAATTC
Asp700I 2105 GAACTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3685 GAACCCCTTC
AvaI 1413 CCCGAG C^[CT]CG[GA]G
4028 CCCGGG
BbsI 260 GAAGAC GAAGAC(2/6)
4202 GAAGAC
BcgI 3583 CGAGTACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1964 GCAGGCCTCTCG
BglI 2910 GCCTGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3072 GCCCGCTGGGC
BlpI 167 GCTGAGC GC^T[ACGT]AGC
2939 GCTGAGC
Bpu1102I 167 GCTGAGC GC^T[ACGT]AGC
2939 GCTGAGC
BpuAI 260 GAAGAC GAAGAC(2/6)
4202 GAAGAC
BsaAI 851 TACGTG [CT]AC^GT[GA]
2635 CACGTG
BsmBI 2766 GAGACG CGTCTC(1/5)
3796 GAGACG
BsoBI 1413 CCCGAG C^[CT]CG[GA]G
4028 CCCGGG
Bsp1407I 311 TGTACA T^GTACA
3663 TGTACA
BspHI 3756 TCATGA T^CATGA
4260 TCATGA
BsrFI 1040 ACCGGT [GA]^CCGG[CT]
2465 ACCGGC
BsrGI 311 TGTACA T^GTACA
3663 TGTACA
Bsu36I 408 CCTCAGG CC^T[ACGT]AGG
2627 CCTGAGG
CelII 167 GCTGAGC GC^T[ACGT]AGC
2939 GCTGAGC
Cfr10I 1040 ACCGGT [GA]^CCGG[CT]
2465 ACCGGC
Eco81I 408 CCTCAGG CC^T[ACGT]AGG
2627 CCTGAGG
EcoNI 607 CCTCAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1517 CCTGCTCCAGG
EcoRI 2272 GAATTC G^AATTC
4247 GAATTC
HindIII 36 AAGCTT A^AGCTT
513 AAGCTT
PflMI 2979 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3896 CCAGAAAGTGG
RcaI 3756 TCATGA T^CATGA
4260 TCATGA
SapI 966 GAAGAGC GCTCTTC(1/4)
2027 GAAGAGC
ScaI 3045 AGTACT AGT^ACT
3585 AGTACT
SspBI 311 TGTACA T^GTACA
3663 TGTACA
Van91I 2979 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3896 CCAGAAAGTGG
XmnI 2105 GAACTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3685 GAACCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI ApaI AspHI AvaII
BalI BamHI BanI BanII BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrBI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII