##################################
# NO CUT ENZYMES (  183 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaII       
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
Cfr13I      ClaI        CpoI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRV       EcoT14I     FauI        
FokI        FseI        FspI        HaeII       HaeIII      
HapII       HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        HpaII       HphI        KasI        
KpnI        KspI        MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MspA1I      
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NspV        PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        Tth111I     Van91I      XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AciI           67  GCGG                 CCGC(-3/-1)
AfaI          719  GTAC                 GT^AC
AseI          830  ATTAAT               AT^TAAT
AsnI          830  ATTAAT               AT^TAAT
AvaI          117  CTCGAG               C^[CT]CG[GA]G
BanI          758  GGTGCC               G^G[CT][GA]CC
BanII         120  GAGCCC               G[GA]GC[CT]^C
BclI          370  TGATCA               T^GATCA
BmyI          120  GAGCCC               G[AGT]GC[ACT]^C
BsmAI         424  GTCTC                GTCTC(1/5)
BsmBI         423  CGTCTC               CGTCTC(1/5)
BsmFI         274  GTCCC                GGGAC(10/14)
BsoBI         117  CTCGAG               C^[CT]CG[GA]G
Bsp1286I      120  GAGCCC               G[AGT]GC[ACT]^C
BsrDI         663  CATTGC               GCAATG(2/0)
Cac8I         222  GCGTGC               GC[ACGT]^[ACGT]GC
CfoI          226  GCGC                 GCG^C
DpnII         371  GATC                 ^GATC
EcoRI         260  GAATTC               G^AATTC
EcoT22I       606  ATGCAT               ATGCA^T
FbaI          370  TGATCA               T^GATCA
HhaI          226  GCGC                 GCG^C
HinP1I        226  GCGC                 G^CGC
MaeII         422  ACGT                 A^CGT
MboI          371  GATC                 ^GATC
MwoI          113  GCTGCTCGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII         371  GATC                 ^GATC
NsiI          606  ATGCAT               ATGCA^T
NspI          353  GCATGT               [GA]CATG^[CT]
PacI          504  TTAATTAA             TTAAT^TAA
PaeR7I        117  CTCGAG               C^TCGAG
PshBI         830  ATTAAT               AT^TAAT
RsaI          719  GTAC                 GT^AC
Sau3AI        371  GATC                 ^GATC
SmlI          117  CTCGAG               C^T[CT][GA]AG
Tsp45I        769  GTCAC                ^GT[GC]AC
XbaI          446  TCTAGA               T^CTAGA
XcmI          230  CCAACTCCCAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          117  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

BbvI          113  GCTGC                GCAGC(8/12)
              724  GCTGC               
BfaI          447  CTAG                 C^TAG
              641  CTAG                
BsaJI          10  CCTCGG               C^C[ACGT][ACGT]GG
              437  CCCTGG              
BseRI          36  CTCCTC               GAGGAG(10/8)
               41  CTCCTC              
BssKI          89  CCAGG                ^CC[ACGT]GG
              438  CCTGG               
BstNI          89  CCAGG                CC^[AT]GG
              438  CCTGG               
DraI          522  TTTAAA               TTT^AAA
              787  TTTAAA              
EarI           46  CTCTTC               CTCTTC(1/4)
              311  GAAGAG              
EcoRII         89  CCAGG                ^CC[AT]GG
              438  CCTGG               
Fnu4HI        113  GCTGC                GC^[ACGT]GC
              724  GCTGC               
ItaI          113  GCTGC                GC^[ACGT]GC
              724  GCTGC               
Ksp632I        46  CTCTTC               CTCTTC(1/4)
              311  GAAGAG              
MaeI          447  CTAG                 C^TAG
              641  CTAG                
MaeIII        581  GTAAC                ^GT[ACGT]AC
              769  GTCAC               
MslI          374  CACACAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              463  CATAAATGTG          
MvaI           89  CCAGG                CC^[AT]GG
              438  CCTGG               
NlaIII         61  CATG                 CATG^
              354  CATG                
NlaIV         210  GGAACC               GG[ACGT]^[ACGT]CC
              758  GGTGCC              
ScrFI          89  CCAGG                CC^[ACGT]GG
              438  CCTGG               
TseI          113  GCTGC                G^C[AT]GC
              724  GCTGC               
TspRI          82  CAGTG                CA[GC]TG[ACGT][ACGT]^
              325  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AcsI        AluI        ApoI        BsiYI       BslI        
DdeI        HinfI       MboII       MnlI        MseI        
TaqI        TfiI        Tru9I       Tsp509I     TthHB8I