##################################
# NO CUT ENZYMES (   89 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
Asp718I     AspEI       AviII       AvrII       BamHI       
BfrI        BlnI        BlpI        Bpu1102I    BsaBI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     CelII       ClaI        CpoI        DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        KspI        MamI        MfeI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshAI       Psp1406I    RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfuI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AcsI          679  AAATTC               [GA]^AATT[CT]
AflIII        800  ACGCGT               A^C[GA][CT]GT
ApoI          679  AAATTC               [GA]^AATT[CT]
Asp700I      2548  GAAGTAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1502  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          137  CCCGGG               C^[CT]CG[GA]G
BalI         1131  TGGCCA               TGG^CCA
BbrPI         788  CACGTG               CAC^GTG
BcgI         2443  CGACTCTTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1471  GTATCC               GTATCC(6/5)
BclI          524  TGATCA               T^GATCA
BglI         2194  GCCCTCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI         1500  GAGACC               GGTCTC(1/5)
BsoBI         137  CCCGGG               C^[CT]CG[GA]G
BspMI         401  ACCTGC               ACCTGC(4/8)
BstAPI       1955  GCAATTTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI         2629  TTTAAA               TTT^AAA
DraIII       2348  CACATCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         2306  CCATGG               C^C[GA][CT]GG
EaeI         1131  TGGCCA               [CT]^GGCC[GA]
EcoNI        2827  CCTTGGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI          524  TGATCA               T^GATCA
MluI          800  ACGCGT               A^CGCGT
MluNI        1131  TGGCCA               TGG^CCA
MscI         1131  TGGCCA               TGG^CCA
NaeI         1085  GCCGGC               GCC^GGC
NcoI         2306  CCATGG               C^CATGG
NgoMIV       1085  GCCGGC               G^CCGGC
NspI         1199  GCATGC               [GA]CATG^[CT]
PflFI        1502  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         788  CACGTG               CAC^GTG
PmlI          788  CACGTG               CAC^GTG
PpuMI         367  GGGTCCT              [GA]G^G[AT]CC[CT]
PstI         2375  CTGCAG               CTGCA^G
PvuI           26  CGATCG               CGAT^CG
SgrAI          82  CACCGGCG             C[GA]^CCGG[CT]G
SmaI          137  CCCGGG               CCC^GGG
SphI         1199  GCATGC               GCATG^C
SspI          262  AATATT               AAT^ATT
StuI          283  AGGCCT               AGG^CCT
Tth111I      1502  GACCTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         2306  CCATGGACACCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         137  CCCGGG               C^CCGGG
XmaI          137  CCCGGG               C^CCGGG
XmnI         2548  GAAGTAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI          744  GTCTAC               GT^[AC][GT]AC
             1508  GTCTAC              
AccIII        931  TCCGGA               T^CCGGA
             1187  TCCGGA              
AlwI          651  GATCC                GGATC(4/5)
              795  GGATC               
AocI          156  CCTCAGG              CC^T[ACGT]AGG
              286  CCTCAGG             
ApaI         1737  GGGCCC               GGGCC^C
             1844  GGGCCC              
AseI         2151  ATTAAT               AT^TAAT
             2609  ATTAAT              
AsnI         2151  ATTAAT               AT^TAAT
             2609  ATTAAT              
BbvCI         371  CCTCAGC              CCTCAGC(-5/-2)
             1906  GCTGAGG             
BfaI         1971  CTAG                 C^TAG
             2191  CTAG                
BglII         541  AGATCT               A^GATCT
             1043  AGATCT              
BsaAI         788  CACGTG               [CT]AC^GT[GA]
             1378  TACGTG              
BseAI         931  TCCGGA               T^CCGGA
             1187  TCCGGA              
BsiEI          26  CGATCG               CG[GA][CT]^CG
              170  CGACCG              
BspEI         931  TCCGGA               T^CCGGA
             1187  TCCGGA              
BsrBI         481  GAGCGG               CCGCTC(-3/-3)
             1143  GAGCGG              
Bsu36I        156  CCTCAGG              CC^T[ACGT]AGG
              286  CCTCAGG             
Eco81I        156  CCTCAGG              CC^T[ACGT]AGG
              286  CCTCAGG             
MaeI         1971  CTAG                 C^TAG
             2191  CTAG                
MroI          931  TCCGGA               T^CCGGA
             1187  TCCGGA              
MslI         1200  CATGCTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1422  CACCTGTGTG          
PflMI         411  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2002  CCAACAGCTGG         
PshBI        2151  ATTAAT               AT^TAAT
             2609  ATTAAT              
SacI          358  GAGCTC               GAGCT^C
             2079  GAGCTC              
SapI         1018  GAAGAGC              GCTCTTC(1/4)
             1068  GAAGAGC             
SfcI         2375  CTGCAG               C^T[GA][CT]AG
             2520  CTATAG              
Van91I        411  CCAGCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2002  CCAACAGCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   98 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AspHI       AvaII       BanI        BanII       
BbeI        BbsI        BbvI        BcnI        BmyI        
BpmI        BpuAI       BsaHI       BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BsrFI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        KasI        Ksp632I     MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NarI        NciI        NdeII       NlaIII      NlaIV       
PleI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfoI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII