##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
Acc65I AclI AflII AgeI AscI
AseI AsnI Asp700I Asp718I AspI
AviII BamHI BbeI BfrI BsiWI
Bsp1407I BspDI BsrBI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI DraI DraIII EagI EclXI
Eco52I EcoO65I EcoRV EcoT22I FseI
FspI HindIII HpaI KasI KpnI
MfeI MluI MunI NarI NdeI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SwaI
Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AatII 4944 GACGTC GACGT^C
AccIII 2111 TCCGGA T^CCGGA
AflIII 589 ACATGT A^C[GA][CT]GT
AhdI 5189 GACTTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1353 GTGCAC G^TGCAC
Aor51HI 2935 AGCGCT AGC^GCT
ApaLI 1353 GTGCAC G^TGCAC
AspEI 5189 GACTTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2984 CCTAGG C^CTAGG
BciVI 4088 GGATAC GTATCC(6/5)
BclI 1762 TGATCA T^GATCA
BlnI 2984 CCTAGG C^CTAGG
BsaBI 183 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2111 TCCGGA T^CCGGA
BsiEI 5000 CGACCG CG[GA][CT]^CG
BspEI 2111 TCCGGA T^CCGGA
BspHI 3502 TCATGA T^CATGA
BspLU11I 589 ACATGT A^CATGT
CpoI 3904 CGGACCG CG^G[AT]CCG
Eam1105I 5189 GACTTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2935 AGCGCT AGC^GCT
EcoRI 111 GAATTC G^AATTC
FbaI 1762 TGATCA T^GATCA
KspI 3338 CCGCGG CCGC^GG
MamI 183 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2111 TCCGGA T^CCGGA
NheI 749 GCTAGC G^CTAGC
RcaI 3502 TCATGA T^CATGA
RsrII 3904 CGGACCG CG^G[AT]CCG
SacII 3338 CCGCGG CCGC^GG
SapI 833 GCTCTTC GCTCTTC(1/4)
SspI 3615 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1776 GTCTAC GT^[AC][GT]AC
4044 GTCTAC
AcsI 111 GAATTC [GA]^AATT[CT]
3152 GAATTT
AcyI 4944 GACGTC G[GA]^CG[CT]C
4990 GACGCC
ApoI 111 GAATTC [GA]^AATT[CT]
3152 GAATTT
BbrPI 510 CACGTG CAC^GTG
3729 CACGTG
BglII 304 AGATCT A^GATCT
1372 AGATCT
BsaAI 510 CACGTG [CT]AC^GT[GA]
3729 CACGTG
BsaHI 4944 GACGTC G[GA]^CG[CT]C
4990 GACGCC
BsaI 3923 GGTCTC GGTCTC(1/5)
4529 GGTCTC
BsaWI 2111 TCCGGA [AT]^CCGG[AT]
5176 TCCGGT
BsmI 802 GCATTC GAATGC(1/-1)
2965 GCATTC
BsrDI 3824 GCAATG GCAATG(2/0)
4239 GCAATG
BssSI 2199 CACGAG CACGAG(-5/-1)
4629 CACGAG
DrdI 561 GACCCAAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4842 GACCAGCTTGTC
Hin1I 4944 GACGTC G[GA]^CG[CT]C
4990 GACGCC
HincII 955 GTCAAC GT[CT]^[GA]AC
1331 GTCAAC
HindII 955 GTCAAC GT[CT]^[GA]AC
1331 GTCAAC
MseI 3613 TTAA T^TAA
5232 TTAA
NaeI 2697 GCCGGC GCC^GGC
2789 GCCGGC
NcoI 982 CCATGG C^CATGG
4817 CCATGG
NgoMIV 2697 GCCGGC G^CCGGC
2789 GCCGGC
PaeR7I 2777 CTCGAG C^TCGAG
3926 CTCGAG
PmaCI 510 CACGTG CAC^GTG
3729 CACGTG
PmlI 510 CACGTG CAC^GTG
3729 CACGTG
SacI 1049 GAGCTC GAGCT^C
3467 GAGCTC
SmaI 3481 CCCGGG CCC^GGG
5125 CCCGGG
SphI 2429 GCATGC GCATG^C
4929 GCATGC
Tru9I 3613 TTAA T^TAA
5232 TTAA
XcmI 55 CCAGGACCCCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1438 CCAGTAGAGTCCTGG
XhoI 2777 CTCGAG C^TCGAG
3926 CTCGAG
XmaCI 3481 CCCGGG C^CCGGG
5125 CCCGGG
XmaI 3481 CCCGGG C^CCGGG
5125 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI ApaI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcgI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XhoII