##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AflIII Alw44I Aor51HI ApaLI
AscI AseI AsnI AspI AvrII
BalI BbeI BciVI BfrI BlnI
BsaHI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BsrBI
BssHII BssSI Bst1107I BstEII BstPI
BstXI BstZ17I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoT22I FauI FseI HaeII Hin1I
KasI KspI MfeI MluI MluNI
MroI MscI MunI NarI NdeI
NotI NruI NsiI PacI PaeR7I
PflFI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfiI SfoI SnaBI
SpeI Sse8387I SspI StuI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 3011 GGTACC G^GTACC
AccII 2839 CGCG CG^CG
AgeI 2649 ACCGGT A^CCGGT
AlwNI 3410 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 3043 GGGCCC GGGCC^C
Asp718I 3011 GGTACC G^GTACC
AvaI 2113 CCCGGG C^[CT]CG[GA]G
AviII 2824 TGCGCA TGC^GCA
BamHI 908 GGATCC G^GATCC
BbrPI 1588 CACGTG CAC^GTG
BcgI 3313 GCAAACACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2703 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2229 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2229 GCTAAGC GC^T[ACGT]AGC
BsaBI 903 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1660 GAGACC GGTCTC(1/5)
BsaWI 2649 ACCGGT [AT]^CCGG[AT]
BsoBI 2113 CCCGGG C^[CT]CG[GA]G
BstUI 2839 CGCG CG^CG
CelII 2229 GCTAAGC GC^T[ACGT]AGC
DsaI 1412 CCATGG C^C[GA][CT]GG
EaeI 2807 TGGCCG [CT]^GGCC[GA]
EcoRI 2814 GAATTC G^AATTC
EcoRV 630 GATATC GAT^ATC
FspI 2824 TGCGCA TGC^GCA
HgaI 411 GACGC GACGC(5/10)
HindIII 3103 AAGCTT A^AGCTT
HpaI 3367 GTTAAC GTT^AAC
KpnI 3011 GGTACC GGTAC^C
MamI 903 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2839 CGCG CG^CG
NaeI 1109 GCCGGC GCC^GGC
NcoI 1412 CCATGG C^CATGG
NgoMIV 1109 GCCGGC G^CCGGC
NheI 3288 GCTAGC G^CTAGC
PflMI 1269 CCATTAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2649 ACCGGT A^CCGGT
PmaCI 1588 CACGTG CAC^GTG
PmlI 1588 CACGTG CAC^GTG
PvuII 2362 CAGCTG CAG^CTG
SacI 2536 GAGCTC GAGCT^C
SapI 1956 GAAGAGC GCTCTTC(1/4)
SexAI 3458 ACCTGGT A^CC[AT]GGT
SgrAI 2648 CACCGGTG C[GA]^CCGG[CT]G
SmaI 2113 CCCGGG CCC^GGG
SphI 2020 GCATGC GCATG^C
Van91I 1269 CCATTAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2113 CCCGGG C^CCGGG
XmaI 2113 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AhdI 3224 GACACTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3438 GACATTCTGTC
AocI 1824 CCTAAGG CC^T[ACGT]AGG
2997 CCTGAGG
AspEI 3224 GACACTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3438 GACATTCTGTC
BbsI 1320 GAAGAC GAAGAC(2/6)
2475 GAAGAC
BbvCI 735 CCTCAGC CCTCAGC(-5/-2)
2868 CCTCAGC
BclI 1399 TGATCA T^GATCA
3061 TGATCA
BpuAI 1320 GAAGAC GAAGAC(2/6)
2475 GAAGAC
BsaAI 373 TACGTG [CT]AC^GT[GA]
1588 CACGTG
BsmFI 3207 GGGAC GGGAC(10/14)
3406 GTCCC
Bsp1407I 239 TGTACA T^GTACA
2878 TGTACA
BspMI 2318 ACCTGC ACCTGC(4/8)
2913 GCAGGT
BsrFI 1109 GCCGGC [GA]^CCGG[CT]
2649 ACCGGT
BsrGI 239 TGTACA T^GTACA
2878 TGTACA
BstAPI 305 GCAAGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2322 GCAGACCTTGC
BstBI 570 TTCGAA TT^CGAA
943 TTCGAA
Bsu36I 1824 CCTAAGG CC^T[ACGT]AGG
2997 CCTGAGG
CfoI 2825 GCGC GCG^C
2840 GCGC
Cfr10I 1109 GCCGGC [GA]^CCGG[CT]
2649 ACCGGT
DraI 996 TTTAAA TTT^AAA
3423 TTTAAA
Eam1105I 3224 GACACTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3438 GACATTCTGTC
Eco81I 1824 CCTAAGG CC^T[ACGT]AGG
2997 CCTGAGG
EcoNI 1439 CCTCTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2997 CCTGAGGGAGG
EcoT14I 466 CCTTGG C^C[AT][AT]GG
1412 CCATGG
FbaI 1399 TGATCA T^GATCA
3061 TGATCA
HhaI 2825 GCGC GCG^C
2840 GCGC
HinP1I 2825 GCGC G^CGC
2840 GCGC
HincII 1019 GTCAAC GT[CT]^[GA]AC
3367 GTTAAC
HindII 1019 GTCAAC GT[CT]^[GA]AC
3367 GTTAAC
MaeII 374 ACGT A^CGT
1589 ACGT
MslI 1182 CACAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2828 CACCACTGTG
MspA1I 2362 CAGCTG C[AC]G^C[GT]G
2871 CAGCGG
NspI 2020 GCATGC [GA]CATG^[CT]
2666 ACATGC
NspV 570 TTCGAA TT^CGAA
943 TTCGAA
PstI 2320 CTGCAG CTGCA^G
2713 CTGCAG
SfuI 570 TTCGAA TT^CGAA
943 TTCGAA
SspBI 239 TGTACA T^GTACA
2878 TGTACA
StyI 466 CCTTGG C^C[AT][AT]GG
1412 CCATGG
XcmI 2196 CCAAGCAATATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3015 CCAGGGAGAACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI AvaII BanI
BanII BbvI BcnI BfaI BglII
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII DraIII EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI