##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspI AviII
BbeI BbrPI BbvCI BcgI BclI
BfrI BglI BsaAI BseAI BsgI
BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BssSI Bst1107I BstAPI BstZ17I
Bsu36I CpoI EagI EclXI Eco47III
Eco52I Eco81I FbaI FseI FspI
KasI KpnI KspI MfeI MluI
MroI MunI NarI NheI NotI
NruI PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SexAI SfiI SfoI SgrAI
SnaBI SphI SspI SwaI Tth111I
XcmI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AflIII 2774 ACATGT A^C[GA][CT]GT
AgeI 358 ACCGGT A^CCGGT
AhdI 1779 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1779 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2528 CCTAGG C^CTAGG
BalI 2936 TGGCCA TGG^CCA
BglII 1857 AGATCT A^GATCT
BlnI 2528 CCTAGG C^CTAGG
BlpI 2228 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2228 GCTCAGC GC^T[ACGT]AGC
BsaBI 2891 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3834 GGTCTC GGTCTC(1/5)
Bsp1407I 3145 TGTACA T^GTACA
BspDI 2683 ATCGAT AT^CGAT
BspLU11I 2774 ACATGT A^CATGT
BsrGI 3145 TGTACA T^GTACA
BssHII 3702 GCGCGC G^CGCGC
BstBI 17 TTCGAA TT^CGAA
BstEII 594 GGTGACC G^GT[ACGT]ACC
BstPI 594 GGTGACC G^GT[ACGT]ACC
CelII 2228 GCTCAGC GC^T[ACGT]AGC
ClaI 2683 ATCGAT AT^CGAT
DraIII 996 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 5 GACCCAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2936 TGGCCA [CT]^GGCC[GA]
Eam1105I 1779 GACTGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 594 GGTGACC G^GT[ACGT]ACC
EcoRI 20 GAATTC G^AATTC
EcoT22I 4040 ATGCAT ATGCA^T
HpaI 1769 GTTAAC GTT^AAC
MamI 2891 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2936 TGGCCA TGG^CCA
MscI 2936 TGGCCA TGG^CCA
NcoI 2557 CCATGG C^CATGG
NsiI 4040 ATGCAT ATGCA^T
NspV 17 TTCGAA TT^CGAA
PinAI 358 ACCGGT A^CCGGT
SacI 2131 GAGCTC GAGCT^C
SbfI 368 CCTGCAGG CCTGCA^GG
SfuI 17 TTCGAA TT^CGAA
SmaI 590 CCCGGG CCC^GGG
SpeI 3373 ACTAGT A^CTAGT
Sse8387I 368 CCTGCAGG CCTGCA^GG
SspBI 3145 TGTACA T^GTACA
StuI 2661 AGGCCT AGG^CCT
XbaI 3972 TCTAGA T^CTAGA
XmaCI 590 CCCGGG C^CCGGG
XmaI 590 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 220 GTAGAC GT^[AC][GT]AC
862 GTCTAC
AccII 2497 CGCG CG^CG
3703 CGCG
AcyI 1696 GACGCC G[GA]^CG[CT]C
3279 GGCGTC
Asp700I 926 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3460 GAATATGTTC
AvaI 590 CCCGGG C^[CT]CG[GA]G
2244 CCCGAG
BamHI 1169 GGATCC G^GATCC
4193 GGATCC
BciVI 4103 GGATAC GTATCC(6/5)
2725 GTATCC
BsaHI 1696 GACGCC G[GA]^CG[CT]C
3279 GGCGTC
BsmI 2713 GAATGC GAATGC(1/-1)
1586 GCATTC
BsoBI 590 CCCGGG C^[CT]CG[GA]G
2244 CCCGAG
BsrDI 1077 CATTGC GCAATG(2/0)
3594 CATTGC
BstUI 2497 CGCG CG^CG
3703 CGCG
EcoRV 651 GATATC GAT^ATC
1308 GATATC
FauI 2667 CCCGC CCCGC(4/6)
2433 GCGGG
HaeII 431 GGCGCT [GA]GCGC^[CT]
499 AGCGCC
Hin1I 1696 GACGCC G[GA]^CG[CT]C
3279 GGCGTC
HincII 1769 GTTAAC GT[CT]^[GA]AC
3353 GTTGAC
HindII 1769 GTTAAC GT[CT]^[GA]AC
3353 GTTGAC
MslI 2310 CATTACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3066 CATAGTCATG
MvnI 2497 CGCG CG^CG
3703 CGCG
NaeI 2125 GCCGGC GCC^GGC
3761 GCCGGC
NdeI 276 CATATG CA^TATG
3924 CATATG
NgoMIV 2125 GCCGGC G^CCGGC
3761 GCCGGC
NspI 52 ACATGC [GA]CATG^[CT]
2774 ACATGT
PflMI 680 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
995 CCACACTGTGG
PpuMI 585 AGGACCC [GA]G^G[AT]CC[CT]
1403 AGGTCCC
SmlI 1774 CTCAAG C^T[CT][GA]AG
3212 CTCAAG
Van91I 680 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
995 CCACACTGTGG
XmnI 926 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3460 GAATATGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AspHI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII