##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BbeI BbrPI
BbvCI BclI BfrI BglII BlnI
BsaAI BsaBI BsaI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspHI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI CfoI
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRV FbaI FseI FspI
HaeII HhaI HinP1I HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I StuI
SwaI TaqI Tsp45I Tth111I TthHB8I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 133 GTATAC GT^[AC][GT]AC
AccII 36 CGCG CG^CG
AcyI 39 GACGCC G[GA]^CG[CT]C
AlwNI 227 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1290 GGGCCC GGGCC^C
Asp700I 531 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 1076 GTCTTC GAAGAC(2/6)
BcgI 212 CGAGCTTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 791 GTATCC GTATCC(6/5)
BcnI 598 CCGGG CC^[GC]GG
BglI 1283 GCCTTATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 478 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 478 GCTCAGC GC^T[ACGT]AGC
BpuAI 1076 GTCTTC GAAGAC(2/6)
BsaHI 39 GACGCC G[GA]^CG[CT]C
BsaWI 52 ACCGGA [AT]^CCGG[AT]
Bsp1407I 1743 TGTACA T^GTACA
BsrGI 1743 TGTACA T^GTACA
Bst1107I 133 GTATAC GTA^TAC
BstEII 1505 GGTTACC G^GT[ACGT]ACC
BstPI 1505 GGTTACC G^GT[ACGT]ACC
BstUI 36 CGCG CG^CG
BstXI 374 CCACAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 133 GTATAC GTA^TAC
CelII 478 GCTCAGC GC^T[ACGT]AGC
DraI 1412 TTTAAA TTT^AAA
DraII 1290 GGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 1535 CGGCCA [CT]^GGCC[GA]
EarI 11 GAAGAG CTCTTC(1/4)
EcoO109I 1290 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1505 GGTTACC G^GT[ACGT]ACC
EcoRI 1557 GAATTC G^AATTC
EcoT22I 1439 ATGCAT ATGCA^T
HgaI 39 GACGC GACGC(5/10)
Hin1I 39 GACGCC G[GA]^CG[CT]C
HindIII 1450 AAGCTT A^AGCTT
Ksp632I 11 GAAGAG CTCTTC(1/4)
MaeII 506 ACGT A^CGT
MspA1I 520 CAGCGG C[AC]G^C[GT]G
MvnI 36 CGCG CG^CG
NciI 598 CCGGG CC^[GC]GG
NsiI 1439 ATGCAT ATGCA^T
PleI 1615 GAGTC GAGTC(4/5)
PshAI 1610 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 199 GAGCTC GAGCT^C
SmlI 1612 CTTGAG C^T[CT][GA]AG
SpeI 1185 ACTAGT A^CTAGT
SspBI 1743 TGTACA T^GTACA
SspI 1645 AATATT AAT^ATT
XmnI 531 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 679 ACATGT A^C[GA][CT]GT
1746 ACATGT
Alw44I 220 GTGCAC G^TGCAC
1816 GTGCAC
ApaLI 220 GTGCAC G^TGCAC
1816 GTGCAC
AvaII 55 GGACC G^G[AT]CC
352 GGTCC
BamHI 1200 GGATCC G^GATCC
1655 GGATCC
BanII 199 GAGCTC G[GA]GC[CT]^C
1290 GGGCCC
BpmI 266 CTGGAG CTGGAG(16/14)
850 CTGGAG
BsgI 112 GTGCAG GTGCAG(16/14)
1115 GTGCAG
BsmFI 241 GTCCC GGGAC(10/14)
353 GTCCC
BspLU11I 679 ACATGT A^CATGT
1746 ACATGT
BspMI 186 ACCTGC ACCTGC(4/8)
403 GCAGGT
BstYI 1200 GGATCC [GA]^GATC[CT]
1655 GGATCC
Cac8I 433 GCTGGC GC[ACGT]^[ACGT]GC
483 GCTTGC
DsaI 287 CCATGG C^C[GA][CT]GG
332 CCATGG
FauI 58 CCCGC CCCGC(4/6)
1090 CCCGC
HapII 53 CCGG C^CGG
598 CCGG
HpaII 53 CCGG C^CGG
598 CCGG
HphI 572 GGTGA GGTGA(8/7)
815 GGTGA
MflI 1200 GGATCC [GA]^GATC[CT]
1655 GGATCC
MslI 588 CACCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1809 CACTGTGGTG
MspI 53 CCGG C^CGG
598 CCGG
NcoI 287 CCATGG C^CATGG
332 CCATGG
NspI 679 ACATGT [GA]CATG^[CT]
1746 ACATGT
TfiI 126 GAATC G^A[AT]TC
1106 GAATC
XhoII 1200 GGATCC [GA]^GATC[CT]
1655 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AspHI BanI BbvI
BfaI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Bsu36I Cfr13I
DdeI DpnII Eco81I EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PflMI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI Tru9I TseI
Tsp509I TspRI Van91I XcmI