##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

AatII       Acc65I      AcyI        AgeI        Alw44I      
ApaLI       AscI        Asp718I     AviII       BbeI        
BsaHI       BsiEI       BsiWI       BsmBI       BsrFI       
BssHII      BstEII      BstPI       Cfr10I      CpoI        
DraIII      DrdI        EagI        EclXI       Eco52I      
EcoNI       EcoO65I     FseI        FspI        HgaI        
Hin1I       HincII      HindII      HpaI        KasI        
KpnI        KspI        MluI        NaeI        NarI        
NgoMIV      NotI        NruI        PacI        PinAI       
PmeI        PvuI        RsrII       SacII       SalI        
SbfI        SfiI        SfoI        SgrAI       Sse8387I    
StuI        SwaI        XbaI        XcmI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccII        5770  CGCG                 CG^CG
AflII         194  CTTAAG               C^TTAAG
AhdI         5897  GACCCTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         5663  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      5837  AGCGCT               AGC^GCT
ApaI         5847  GGGCCC               GGGCC^C
AseI         4016  ATTAAT               AT^TAAT
AsnI         4016  ATTAAT               AT^TAAT
AspEI        5897  GACCCTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         5400  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI         605  CACGTG               CAC^GTG
BfrI          194  CTTAAG               C^TTAAG
BglII        1306  AGATCT               A^GATCT
BlpI         5618  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     5618  GCTCAGC              GC^T[ACGT]AGC
BsaBI        1046  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         4853  GAGACC               GGTCTC(1/5)
BspDI        1932  ATCGAT               AT^CGAT
BspHI        3358  TCATGA               T^CATGA
BspLU11I      388  ACATGT               A^CATGT
BspMI        3730  ACCTGC               ACCTGC(4/8)
BsrBI        2743  CCGCTC               CCGCTC(-3/-3)
BssSI        2859  CACGAG               CACGAG(-5/-1)
Bst1107I     4638  GTATAC               GTA^TAC
BstUI        5770  CGCG                 CG^CG
BstZ17I      4638  GTATAC               GTA^TAC
Bsu36I       5663  CCTGAGG              CC^T[ACGT]AGG
CelII        5618  GCTCAGC              GC^T[ACGT]AGC
ClaI         1932  ATCGAT               AT^CGAT
Eam1105I     5897  GACCCTCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     5837  AGCGCT               AGC^GCT
Eco81I       5663  CCTGAGG              CC^T[ACGT]AGG
EcoRI         877  GAATTC               G^AATTC
EcoRV         600  GATATC               GAT^ATC
EcoT22I      1322  ATGCAT               ATGCA^T
MamI         1046  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1592  CAATTG               C^AATTG
MunI         1592  CAATTG               C^AATTG
MvnI         5770  CGCG                 CG^CG
NheI         6243  GCTAGC               G^CTAGC
NsiI         1322  ATGCAT               ATGCA^T
PaeR7I       5690  CTCGAG               C^TCGAG
PflFI        5400  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI         605  CACGTG               CAC^GTG
PmlI          605  CACGTG               CAC^GTG
PshAI        1729  GACCAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        4016  ATTAAT               AT^TAAT
RcaI         3358  TCATGA               T^CATGA
SexAI        5401  ACCTGGT              A^CC[AT]GGT
SmaI         3471  CCCGGG               CCC^GGG
SnaBI        3132  TACGTA               TAC^GTA
SpeI          581  ACTAGT               A^CTAGT
Tth111I      5400  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         5690  CTCGAG               C^TCGAG
XmaCI        3471  CCCGGG               C^CCGGG
XmaI         3471  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI          384  GTAGAC               GT^[AC][GT]AC
             4638  GTATAC              
AccIII       4004  TCCGGA               T^CCGGA
             4492  TCCGGA              
AclI         1356  AACGTT               AA^CGTT
             2167  AACGTT              
Asp700I      2423  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4582  GAATAGCTTC          
AvrII         659  CCTAGG               C^CTAGG
             5926  CCTAGG              
BamHI        2059  GGATCC               G^GATCC
             5603  GGATCC              
BcgI         4702  CGAGCATCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              306  GCATCAAAATCG        
BciVI        3788  GGATAC               GTATCC(6/5)
              476  GTATCC              
BglI          425  GCCTTTCGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             5260  GCCTCACAGGC         
BlnI          659  CCTAGG               C^CTAGG
             5926  CCTAGG              
BpmI         4763  CTCCAG               CTGGAG(16/14)
             1632  CTGGAG              
BsaWI        4004  TCCGGA               [AT]^CCGG[AT]
             4492  TCCGGA              
BseAI        4004  TCCGGA               T^CCGGA
             4492  TCCGGA              
Bsp1407I     2872  TGTACA               T^GTACA
             5287  TGTACA              
BspEI        4004  TCCGGA               T^CCGGA
             4492  TCCGGA              
BsrGI        2872  TGTACA               T^GTACA
             5287  TGTACA              
BstBI        3045  TTCGAA               TT^CGAA
             4026  TTCGAA              
BstXI        2960  CCAGTAGCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3568  CCAGAATGATGG        
MroI         4004  TCCGGA               T^CCGGA
             4492  TCCGGA              
NspV         3045  TTCGAA               TT^CGAA
             4026  TTCGAA              
PflMI        2019  CCATACCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2536  CCAGGCCATGG         
PpuMI        4420  GGGACCC              [GA]G^G[AT]CC[CT]
             5398  AGGACCT             
Psp1406I     1356  AACGTT               AA^CGTT
             2167  AACGTT              
PvuII         452  CAGCTG               CAG^CTG
             2123  CAGCTG              
SapI          156  GAAGAGC              GCTCTTC(1/4)
             1420  GAAGAGC             
SfuI         3045  TTCGAA               TT^CGAA
             4026  TTCGAA              
SphI          447  GCATGC               GCATG^C
             1324  GCATGC              
SspBI        2872  TGTACA               T^GTACA
             5287  TGTACA              
SspI         1213  AATATT               AAT^ATT
             6077  AATATT              
Van91I       2019  CCATACCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2536  CCAGGCCATGG         
XmnI         2423  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4582  GAATAGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwI        AlwNI       ApoI        AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvCI       BbvI        BclI        BcnI        BfaI        
BmyI        BpuAI       BsaAI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        BsoBI       Bsp1286I    BsrDI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
FbaI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NcoI        NdeI        NdeII       NlaIII      NlaIV       
NspI        PleI        PstI        RsaI        SacI        
Sau3AI      Sau96I      ScaI        ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII