##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI Asp718I AspEI AviII
AvrII BamHI BbeI BbrPI BglI
BlnI BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspLU11I
BsrBI BssHII BssSI Bsu36I ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoT22I FseI
FspI Hin1I KasI KpnI KspI
MfeI MroI MunI NarI NotI
NruI NsiI PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI Van91I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccI 5882 GTATAC GT^[AC][GT]AC
Aor51HI 2262 AGCGCT AGC^GCT
BanI 61 GGTGCC G^G[CT][GA]CC
BcgI 5717 GCAGGAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 347 GGATAC GTATCC(6/5)
BcnI 249 CCGGG CC^[GC]GG
BlpI 3332 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3332 GCTCAGC GC^T[ACGT]AGC
BsaI 5611 GAGACC GGTCTC(1/5)
BspMI 608 GCAGGT ACCTGC(4/8)
Bst1107I 5882 GTATAC GTA^TAC
BstBI 1745 TTCGAA TT^CGAA
BstEII 4792 GGTTACC G^GT[ACGT]ACC
BstPI 4792 GGTTACC G^GT[ACGT]ACC
BstXI 5569 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 5882 GTATAC GTA^TAC
CelII 3332 GCTCAGC GC^T[ACGT]AGC
DrdI 4052 GACATACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2262 AGCGCT AGC^GCT
EcoO65I 4792 GGTTACC G^GT[ACGT]ACC
HindIII 3594 AAGCTT A^AGCTT
HpaI 6081 GTTAAC GTT^AAC
MluI 1528 ACGCGT A^CGCGT
NaeI 8 GCCGGC GCC^GGC
NciI 249 CCGGG CC^[GC]GG
NgoMIV 8 GCCGGC G^CCGGC
NspV 1745 TTCGAA TT^CGAA
PshAI 1699 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 388 GAAGAGC GCTCTTC(1/4)
SfuI 1745 TTCGAA TT^CGAA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 1529 CGCG CG^CG
5681 CGCG
AflIII 788 ACACGT A^C[GA][CT]GT
1528 ACGCGT
AspI 38 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5802 GACTTAGTC
AvaI 292 CCCGAG C^[CT]CG[GA]G
302 CCCGAG
BalI 5061 TGGCCA TGG^CCA
5621 TGGCCA
BclI 2980 TGATCA T^GATCA
6435 TGATCA
BsaBI 983 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5018 GATATAAATC
BsmBI 235 CGTCTC CGTCTC(1/5)
5641 CGTCTC
BsoBI 292 CCCGAG C^[CT]CG[GA]G
302 CCCGAG
BsrFI 8 GCCGGC [GA]^CCGG[CT]
5614 ACCGGC
BstUI 1529 CGCG CG^CG
5681 CGCG
Cfr10I 8 GCCGGC [GA]^CCGG[CT]
5614 ACCGGC
EaeI 5061 TGGCCA [CT]^GGCC[GA]
5621 TGGCCA
EcoRV 1172 GATATC GAT^ATC
2069 GATATC
FbaI 2980 TGATCA T^GATCA
6435 TGATCA
HaeII 11 GGCGCT [GA]GCGC^[CT]
2262 AGCGCT
HgaI 112 GACGC GACGC(5/10)
234 GCGTC
MamI 983 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5018 GATATAAATC
MluNI 5061 TGGCCA TGG^CCA
5621 TGGCCA
MscI 5061 TGGCCA TGG^CCA
5621 TGGCCA
MvnI 1529 CGCG CG^CG
5681 CGCG
NcoI 4818 CCATGG C^CATGG
5323 CCATGG
NheI 1691 GCTAGC G^CTAGC
1717 GCTAGC
NspI 669 ACATGC [GA]CATG^[CT]
4370 GCATGT
PflFI 38 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5802 GACTTAGTC
PpuMI 51 GGGTCCC [GA]G^G[AT]CC[CT]
207 GGGTCCC
PvuII 843 CAGCTG CAG^CTG
5991 CAGCTG
ScaI 452 AGTACT AGT^ACT
1077 AGTACT
Tth111I 38 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5802 GACTTAGTC
XbaI 3995 TCTAGA T^CTAGA
4633 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI ApoI AseI AsnI
Asp700I AspHI AvaII BanII BbsI
BbvCI BbvI BfaI BfrI BglII
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspHI
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeI NdeII
NlaIII NlaIV PleI PshBI PstI
RcaI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI