##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccII AclI Aor51HI ApaI
AscI AviII BamHI BbeI BbrPI
BcnI BsiWI BsmBI BsrBI BssHII
BssSI BstUI CpoI DrdI EagI
EclXI Eco47III Eco52I FseI FspI
KasI KspI MfeI MluI MunI
MvnI NaeI NarI NciI NgoMIV
NotI NruI PacI PaeR7I PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI Sse8387I StuI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 2473 GGTACC G^GTACC
AccIII 1322 TCCGGA T^CCGGA
AgeI 5371 ACCGGT A^CCGGT
Alw44I 672 GTGCAC G^TGCAC
ApaLI 672 GTGCAC G^TGCAC
Asp718I 2473 GGTACC G^GTACC
AspI 1652 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 5958 CCTAGG C^CTAGG
BcgI 986 CGACGATTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1230 GTATCC GTATCC(6/5)
BglI 602 GCCCATTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 5958 CCTAGG C^CTAGG
BlpI 1624 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1624 GCTGAGC GC^T[ACGT]AGC
BsaAI 4627 TACGTG [CT]AC^GT[GA]
BseAI 1322 TCCGGA T^CCGGA
BsiEI 2916 CGGTCG CG[GA][CT]^CG
BsmI 6119 GAATGC GAATGC(1/-1)
BspDI 3129 ATCGAT AT^CGAT
BspEI 1322 TCCGGA T^CCGGA
BspLU11I 6673 ACATGT A^CATGT
BspMI 2074 GCAGGT ACCTGC(4/8)
BsrFI 5371 ACCGGT [GA]^CCGG[CT]
BstBI 389 TTCGAA TT^CGAA
BstXI 3831 CCAGAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1624 GCTGAGC GC^T[ACGT]AGC
Cfr10I 5371 ACCGGT [GA]^CCGG[CT]
ClaI 3129 ATCGAT AT^CGAT
DraII 2538 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 1303 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 2530 CCTAGTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2538 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 822 GATATC GAT^ATC
HaeII 867 GGCGCT [GA]GCGC^[CT]
HpaI 6582 GTTAAC GTT^AAC
KpnI 2473 GGTACC GGTAC^C
MroI 1322 TCCGGA T^CCGGA
NcoI 5664 CCATGG C^CATGG
NdeI 1851 CATATG CA^TATG
NheI 6002 GCTAGC G^CTAGC
NspV 389 TTCGAA TT^CGAA
PflFI 1652 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 5371 ACCGGT A^CCGGT
PpuMI 2538 AGGACCT [GA]G^G[AT]CC[CT]
PstI 3649 CTGCAG CTGCA^G
PvuII 1246 CAGCTG CAG^CTG
SacI 1711 GAGCTC GAGCT^C
ScaI 2953 AGTACT AGT^ACT
SfuI 389 TTCGAA TT^CGAA
SpeI 5756 ACTAGT A^CTAGT
Tth111I 1652 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2597 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 1846 GGCGTC G[GA]^CG[CT]C
3597 GGCGTC
AflII 5271 CTTAAG C^TTAAG
6867 CTTAAG
AflIII 1976 ACGTGT A^C[GA][CT]GT
6673 ACATGT
AhdI 5755 GACTAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6044 GACCACCAGTC
AlwNI 865 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
5496 CAGAATCTG
AspEI 5755 GACTAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6044 GACCACCAGTC
AvaI 3589 CCCGAG C^[CT]CG[GA]G
3696 CCCGAG
BbvCI 3687 CCTCAGC CCTCAGC(-5/-2)
1877 GCTGAGG
BclI 3810 TGATCA T^GATCA
4713 TGATCA
BfrI 5271 CTTAAG C^TTAAG
6867 CTTAAG
BsaHI 1846 GGCGTC G[GA]^CG[CT]C
3597 GGCGTC
BsgI 1896 GTGCAG GTGCAG(16/14)
3625 GTGCAG
BsoBI 3589 CCCGAG C^[CT]CG[GA]G
3696 CCCGAG
Bst1107I 2393 GTATAC GTA^TAC
5846 GTATAC
BstAPI 2159 GCAGCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4151 GCATGTAATGC
BstEII 2379 GGTGACC G^GT[ACGT]ACC
2839 GGTAACC
BstPI 2379 GGTGACC G^GT[ACGT]ACC
2839 GGTAACC
BstZ17I 2393 GTATAC GTA^TAC
5846 GTATAC
Eam1105I 5755 GACTAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6044 GACCACCAGTC
EcoO65I 2379 GGTGACC G^GT[ACGT]ACC
2839 GGTAACC
FbaI 3810 TGATCA T^GATCA
4713 TGATCA
HgaI 1847 GCGTC GACGC(5/10)
3598 GCGTC
Hin1I 1846 GGCGTC G[GA]^CG[CT]C
3597 GGCGTC
MspA1I 1217 CAGCGG C[AC]G^C[GT]G
1246 CAGCTG
SphI 1026 GCATGC GCATG^C
4147 GCATGC
XcmI 1225 CCAGTGTATCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1284 CCAGCATCAACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AocI ApoI AseI AsnI
Asp700I AspHI AvaII BalI BanI
BanII BbsI BbvI BfaI BglII
BmyI BpmI BpuAI BsaBI BsaI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspHI BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DsaI EaeI EarI Eco81I EcoRI
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PshBI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI