##################################
# NO CUT ENZYMES (   45 enzymes) #
##################################

AatII       AccIII      AclI        Aor51HI     AscI        
BamHI       BciVI       BseAI       BspDI       BspEI       
BspHI       BssHII      BstBI       ClaI        CpoI        
DrdI        Eco47III    EcoRI       EcoRV       FseI        
HindIII     KspI        MfeI        MroI        MunI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NspV        PacI        PmeI        Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
ScaI        SfuI        SgrAI       SnaBI       SwaI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AflII        5282  CTTAAG               C^TTAAG
AgeI         4821  ACCGGT               A^CCGGT
AhdI         1442  GACGTGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI         4119  ATTAAT               AT^TAAT
AsnI         4119  ATTAAT               AT^TAAT
Asp700I      2749  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1442  GACGTGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI         2891  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII         169  CCTAGG               C^CTAGG
BbeI          845  GGCGCC               GGCGC^C
BbrPI        2831  CACGTG               CAC^GTG
BclI         3423  TGATCA               T^GATCA
BfrI         5282  CTTAAG               C^TTAAG
BlnI          169  CCTAGG               C^CTAGG
BsaBI        1597  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI         721  CGTCTC               CGTCTC(1/5)
Bsp1407I      672  TGTACA               T^GTACA
BsrBI        3128  GAGCGG               CCGCTC(-3/-3)
BsrFI        4821  ACCGGT               [GA]^CCGG[CT]
BsrGI         672  TGTACA               T^GTACA
Bst1107I     6005  GTATAC               GTA^TAC
BstAPI        859  GCACCAAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I      6005  GTATAC               GTA^TAC
Cfr10I       4821  ACCGGT               [GA]^CCGG[CT]
EagI         1498  CGGCCG               C^GGCCG
Eam1105I     1442  GACGTGTGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1498  CGGCCG               C^GGCCG
Eco52I       1498  CGGCCG               C^GGCCG
EcoT22I      5633  ATGCAT               ATGCA^T
FbaI         3423  TGATCA               T^GATCA
HpaI         5903  GTTAAC               GTT^AAC
KasI          845  GGCGCC               G^GCGCC
MamI         1597  GATGAACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI          246  ACGCGT               A^CGCGT
NarI          845  GGCGCC               GG^CGCC
NsiI         5633  ATGCAT               ATGCA^T
PflFI        2891  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI        4821  ACCGGT               A^CCGGT
PmaCI        2831  CACGTG               CAC^GTG
PmlI         2831  CACGTG               CAC^GTG
PshBI        4119  ATTAAT               AT^TAAT
SapI         2536  GCTCTTC              GCTCTTC(1/4)
SbfI         5754  CCTGCAGG             CCTGCA^GG
SfiI         2515  GGCCCTGGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          845  GGCGCC               GGC^GCC
SpeI         5172  ACTAGT               A^CTAGT
Sse8387I     5754  CCTGCAGG             CCTGCA^GG
SspBI         672  TGTACA               T^GTACA
Tth111I      2891  GACTGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3894  TCTAGA               T^CTAGA
XmnI         2749  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

Acc65I       1722  GGTACC               G^GTACC
             2874  GGTACC              
AccI         5168  GTAGAC               GT^[AC][GT]AC
             6005  GTATAC              
AcsI         5241  GAATTT               [GA]^AATT[CT]
             5950  AAATTT              
AcyI          845  GGCGCC               G[GA]^CG[CT]C
             1702  GGCGTC              
Alw44I       1505  GTGCAC               G^TGCAC
             5516  GTGCAC              
ApaLI        1505  GTGCAC               G^TGCAC
             5516  GTGCAC              
ApoI         5241  GAATTT               [GA]^AATT[CT]
             5950  AAATTT              
Asp718I      1722  GGTACC               G^GTACC
             2874  GGTACC              
BbvCI        2263  GCTGAGG              CCTCAGC(-5/-2)
             3448  GCTGAGG             
BcgI          242  CGACACGCGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2082  GCATCCTGCTCG        
BsaHI         845  GGCGCC               G[GA]^CG[CT]C
             1702  GGCGTC              
BsiEI        1498  CGGCCG               CG[GA][CT]^CG
             1880  CGGTCG              
BsiWI        1545  CGTACG               C^GTACG
             1896  CGTACG              
BspLU11I     1323  ACATGT               A^CATGT
             4210  ACATGT              
BstEII       1034  GGTGACC              G^GT[ACGT]ACC
             3361  GGTTACC             
BstPI        1034  GGTGACC              G^GT[ACGT]ACC
             3361  GGTTACC             
DraI         4106  TTTAAA               TTT^AAA
             5785  TTTAAA              
EcoO65I      1034  GGTGACC              G^GT[ACGT]ACC
             3361  GGTTACC             
Hin1I         845  GGCGCC               G[GA]^CG[CT]C
             1702  GGCGTC              
KpnI         1722  GGTACC               GGTAC^C
             2874  GGTACC              
PaeR7I       1727  CTCGAG               C^TCGAG
             2197  CTCGAG              
PshAI        1795  GACCCACGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             4222  GACTACTGTC          
SacI         1181  GAGCTC               GAGCT^C
             3014  GAGCTC              
SspI         5558  AATATT               AAT^ATT
             6092  AATATT              
XhoI         1727  CTCGAG               C^TCGAG
             2197  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  137 enzymes) #
#########################################

AccII       AciI        AfaI        AflIII      AluI        
AlwI        AlwNI       AocI        ApaI        AspHI       
AvaI        AvaII       AviII       BalI        BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BglI        BglII       BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaI        BsaJI       
BsaWI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    BspMI       BsrDI       BsrI        BssKI       
BssSI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EarI        Eco81I      EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SexAI       SfaNI       SfcI        SmaI        
SmlI        SphI        StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI