##################################
# NO CUT ENZYMES (   84 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      Aor51HI     ApaLI       
AscI        Asp718I     AspEI       AviII       AvrII       
BbsI        BcgI        BfrI        BlnI        BlpI        
Bpu1102I    BpuAI       BsaBI       BseAI       BsiEI       
BsiWI       BsmI        BspDI       BspEI       BspHI       
BsrBI       BsrFI       BssHII      BstBI       CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoT22I     FauI        FseI        FspI        
KpnI        KspI        MamI        MfeI        MroI        
MunI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmeI        Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcyI           13  GGCGCC               G[GA]^CG[CT]C
ApaI         3788  GGGCCC               GGGCC^C
AseI         4034  ATTAAT               AT^TAAT
AsnI         4034  ATTAAT               AT^TAAT
AspHI          38  GTGCTC               G[AT]GC[AT]^C
AspI         2775  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        3612  GGATCC               G^GATCC
BbeI           13  GGCGCC               GGCGC^C
BbrPI        1116  CACGTG               CAC^GTG
BciVI        1745  GGATAC               GTATCC(6/5)
BglII        2578  AGATCT               A^GATCT
BsaHI          13  GGCGCC               G[GA]^CG[CT]C
BsaI         3386  GGTCTC               GGTCTC(1/5)
BsaWI        3895  TCCGGT               [AT]^CCGG[AT]
BsgI          474  CTGCAC               GTGCAG(16/14)
BsiHKAI        38  GTGCTC               G[AT]GC[AT]^C
BsmBI        1495  GAGACG               CGTCTC(1/5)
Bsp1407I     1852  TGTACA               T^GTACA
BspLU11I     4132  ACATGT               A^CATGT
BsrGI        1852  TGTACA               T^GTACA
BssSI        2919  CACGAG               CACGAG(-5/-1)
BstEII       2399  GGTGACC              G^GT[ACGT]ACC
BstPI        2399  GGTGACC              G^GT[ACGT]ACC
EcoO65I      2399  GGTGACC              G^GT[ACGT]ACC
EcoRV        2208  GATATC               GAT^ATC
Hin1I          13  GGCGCC               G[GA]^CG[CT]C
HindIII      2728  AAGCTT               A^AGCTT
KasI           13  GGCGCC               G^GCGCC
MluI          946  ACGCGT               A^CGCGT
NarI           13  GGCGCC               GG^CGCC
PaeR7I       3236  CTCGAG               C^TCGAG
PflFI        2775  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        1116  CACGTG               CAC^GTG
PmlI         1116  CACGTG               CAC^GTG
PshBI        4034  ATTAAT               AT^TAAT
SapI         3843  GAAGAGC              GCTCTTC(1/4)
ScaI         1317  AGTACT               AGT^ACT
SfoI           13  GGCGCC               GGC^GCC
SphI          985  GCATGC               GCATG^C
SspBI        1852  TGTACA               T^GTACA
Tth111I      2775  GACAAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         3236  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AccII         947  CGCG                 CG^CG
             1637  CGCG                
BbvCI        2325  CCTCAGC              CCTCAGC(-5/-2)
             2352  GCTGAGG             
BclI         1922  TGATCA               T^GATCA
             2716  TGATCA              
BglI         1077  GCCTATACGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3580  GCCTCCCAGGC         
Bst1107I      335  GTATAC               GTA^TAC
             3678  GTATAC              
BstUI         947  CGCG                 CG^CG
             1637  CGCG                
BstZ17I       335  GTATAC               GTA^TAC
             3678  GTATAC              
CfoI           14  GCGC                 GCG^C
             2595  GCGC                
EcoNI        1699  CCTGCCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1976  CCTTATCAAGG         
EcoRI        1309  GAATTC               G^AATTC
             2432  GAATTC              
FbaI         1922  TGATCA               T^GATCA
             2716  TGATCA              
HaeII          13  GGCGCC               [GA]GCGC^[CT]
             2594  GGCGCT              
HhaI           14  GCGC                 GCG^C
             2595  GCGC                
HinP1I         14  GCGC                 G^CGC
             2595  GCGC                
HpaI          798  GTTAAC               GTT^AAC
             4158  GTTAAC              
MvnI          947  CGCG                 CG^CG
             1637  CGCG                
PpuMI         805  GGGACCC              [GA]G^G[AT]CC[CT]
             1249  AGGTCCC             
SmaI         1474  CCCGGG               CCC^GGG
             2764  CCCGGG              
SmlI         1175  CTTGAG               C^T[CT][GA]AG
             3236  CTCGAG              
SspI          642  AATATT               AAT^ATT
             4037  AATATT              
XmaCI        1474  CCCGGG               C^CCGGG
             2764  CCCGGG              
XmaI         1474  CCCGGG               C^CCGGG
             2764  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  110 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AflIII      
AluI        AlwI        AlwNI       AocI        ApoI        
Asp700I     AvaI        AvaII       BalI        BanI        
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaAI       BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrDI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstXI       BstYI       Bsu36I      
Cac8I       Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        Eco81I      EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HgaI        HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflMI       PleI        PshAI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoII       XmnI