##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AccIII AflIII AgeI AhdI ApaI
AscI AspEI AspI AviII AvrII
BbrPI BglII BlnI BsaAI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Cfr10I ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HpaI KspI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NotI
NsiI NspI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SfiI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SspI SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1154 GTAGAC GT^[AC][GT]AC
AclI 3364 AACGTT AA^CGTT
AflII 3506 CTTAAG C^TTAAG
Alw44I 3255 GTGCAC G^TGCAC
Aor51HI 115 AGCGCT AGC^GCT
ApaLI 3255 GTGCAC G^TGCAC
AspHI 3255 GTGCAC G[AT]GC[AT]^C
BbeI 2466 GGCGCC GGCGC^C
BciVI 1035 GTATCC GTATCC(6/5)
BclI 1054 TGATCA T^GATCA
BfrI 3506 CTTAAG C^TTAAG
BsaWI 3533 TCCGGT [AT]^CCGG[AT]
BsiHKAI 3255 GTGCAC G[AT]GC[AT]^C
Eco47III 115 AGCGCT AGC^GCT
EcoNI 3809 CCTCCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1054 TGATCA T^GATCA
KasI 2466 GGCGCC G^GCGCC
NarI 2466 GGCGCC GG^CGCC
NheI 3191 GCTAGC G^CTAGC
NruI 6268 TCGCGA TCG^CGA
PaeR7I 169 CTCGAG C^TCGAG
PshAI 4771 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3364 AACGTT AA^CGTT
SfoI 2466 GGCGCC GGC^GCC
XhoI 169 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 3022 GACGTC GACGT^C
4084 GACGTC
AccII 52 CGCG CG^CG
6269 CGCG
AseI 1061 ATTAAT AT^TAAT
1265 ATTAAT
AsnI 1061 ATTAAT AT^TAAT
1265 ATTAAT
Asp700I 3987 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4610 GAACCTGTTC
BsaBI 2114 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2531 GATCAGCATC
BsrBI 6150 CCGCTC CCGCTC(-3/-3)
86 GAGCGG
BstUI 52 CGCG CG^CG
6269 CGCG
BstXI 5860 CCAAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
6401 CCAGGGTTTTGG
DrdI 2290 GACCACAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4511 GACAGTGAGGTC
HaeII 115 AGCGCT [GA]GCGC^[CT]
2466 GGCGCC
HgaI 3749 GACGC GACGC(5/10)
1661 GCGTC
HincII 1808 GTCAAC GT[CT]^[GA]AC
3454 GTTGAC
HindII 1808 GTCAAC GT[CT]^[GA]AC
3454 GTTGAC
MamI 2114 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2531 GATCAGCATC
MvnI 52 CGCG CG^CG
6269 CGCG
NcoI 543 CCATGG C^CATGG
1140 CCATGG
PshBI 1061 ATTAAT AT^TAAT
1265 ATTAAT
StuI 5175 AGGCCT AGG^CCT
6441 AGGCCT
XbaI 2515 TCTAGA T^CTAGA
4543 TCTAGA
XmnI 3987 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4610 GAACCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
Acc65I AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
Asp718I AvaI AvaII BalI BamHI
BanI BanII BbsI BbvCI BbvI
BcgI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SexAI SfaNI SfcI SmlI SpeI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII