##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AflII AgeI AhdI Aor51HI
AscI AspEI AspI AvrII BalI
BbrPI BbvCI BfrI BlnI BsaAI
BsiWI Bsp1407I BspDI BsrGI BssHII
BstEII BstPI ClaI CpoI Eam1105I
Eco47III EcoO65I FseI HpaI MfeI
MluI MluNI MscI MunI NdeI
NotI NruI PflFI PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SacI SalI SbfI ScaI SfiI
SgrAI SnaBI Sse8387I SspBI StuI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 4160 GTATAC GT^[AC][GT]AC
AccIII 1059 TCCGGA T^CCGGA
AclI 638 AACGTT AA^CGTT
ApaI 1295 GGGCCC GGGCC^C
Asp700I 2692 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1164 TGCGCA TGC^GCA
BamHI 2555 GGATCC G^GATCC
BcgI 1951 CGAGATGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2402 AGATCT A^GATCT
BlpI 3546 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3546 GCTGAGC GC^T[ACGT]AGC
BsaBI 4716 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1059 TCCGGA T^CCGGA
BsiEI 177 CGGCCG CG[GA][CT]^CG
BsmBI 3970 GAGACG CGTCTC(1/5)
BspEI 1059 TCCGGA T^CCGGA
BspHI 4177 TCATGA T^CATGA
BspLU11I 2247 ACATGT A^CATGT
Bst1107I 4160 GTATAC GTA^TAC
BstAPI 4507 GCACCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 313 TTCGAA TT^CGAA
BstZ17I 4160 GTATAC GTA^TAC
CelII 3546 GCTGAGC GC^T[ACGT]AGC
EagI 177 CGGCCG C^GGCCG
EclXI 177 CGGCCG C^GGCCG
Eco52I 177 CGGCCG C^GGCCG
EcoRI 1855 GAATTC G^AATTC
EcoRV 1746 GATATC GAT^ATC
FspI 1164 TGCGCA TGC^GCA
HincII 1701 GTCAAC GT[CT]^[GA]AC
HindII 1701 GTCAAC GT[CT]^[GA]AC
KspI 265 CCGCGG CCGC^GG
MamI 4716 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1059 TCCGGA T^CCGGA
NaeI 63 GCCGGC GCC^GGC
NgoMIV 63 GCCGGC G^CCGGC
NspV 313 TTCGAA TT^CGAA
PacI 664 TTAATTAA TTAAT^TAA
Psp1406I 638 AACGTT AA^CGTT
PvuII 4671 CAGCTG CAG^CTG
RcaI 4177 TCATGA T^CATGA
SacII 265 CCGCGG CCGC^GG
SexAI 1532 ACCTGGT A^CC[AT]GGT
SfuI 313 TTCGAA TT^CGAA
SpeI 3831 ACTAGT A^CTAGT
XmnI 2692 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1620 GGTACC G^GTACC
1728 GGTACC
AflIII 2247 ACATGT A^C[GA][CT]GT
3772 ACACGT
AocI 2893 CCTGAGG CC^T[ACGT]AGG
3647 CCTGAGG
AseI 2470 ATTAAT AT^TAAT
3189 ATTAAT
AsnI 2470 ATTAAT AT^TAAT
3189 ATTAAT
Asp718I 1620 GGTACC G^GTACC
1728 GGTACC
BbeI 36 GGCGCC GGCGC^C
215 GGCGCC
BciVI 3629 GGATAC GTATCC(6/5)
3390 GTATCC
BclI 1712 TGATCA T^GATCA
3168 TGATCA
BsaI 929 GGTCTC GGTCTC(1/5)
4421 GGTCTC
BsaWI 1059 TCCGGA [AT]^CCGG[AT]
3047 ACCGGA
BsrBI 4434 CCGCTC CCGCTC(-3/-3)
3430 GAGCGG
BsrFI 63 GCCGGC [GA]^CCGG[CT]
4418 GCCGGT
BssSI 2151 CACGAG CACGAG(-5/-1)
3624 CACGAG
BstXI 2173 CCAAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4328 CCAGTTACTTGG
Bsu36I 2893 CCTGAGG CC^T[ACGT]AGG
3647 CCTGAGG
Cfr10I 63 GCCGGC [GA]^CCGG[CT]
4418 GCCGGT
DraIII 3064 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
4247 CACTTTGTG
DrdI 3025 GACATCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4542 GACGGGACAGTC
EaeI 61 TGGCCG [CT]^GGCC[GA]
177 CGGCCG
Eco81I 2893 CCTGAGG CC^T[ACGT]AGG
3647 CCTGAGG
EcoNI 1299 CCTCTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2982 CCTGGGAGAGG
EcoT22I 1955 ATGCAT ATGCA^T
2070 ATGCAT
FbaI 1712 TGATCA T^GATCA
3168 TGATCA
HindIII 955 AAGCTT A^AGCTT
3865 AAGCTT
KasI 36 GGCGCC G^GCGCC
215 GGCGCC
KpnI 1620 GGTACC GGTAC^C
1728 GGTACC
NarI 36 GGCGCC GG^CGCC
215 GGCGCC
NheI 865 GCTAGC G^CTAGC
4409 GCTAGC
NsiI 1955 ATGCAT ATGCA^T
2070 ATGCAT
PaeR7I 989 CTCGAG C^TCGAG
3148 CTCGAG
PflMI 2580 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3063 CCACTTGGTGG
PpuMI 3224 AGGACCT [GA]G^G[AT]CC[CT]
4611 AGGACCT
PshBI 2470 ATTAAT AT^TAAT
3189 ATTAAT
SfoI 36 GGCGCC GGC^GCC
215 GGCGCC
SmaI 151 CCCGGG CCC^GGG
198 CCCGGG
SphI 1957 GCATGC GCATG^C
4145 GCATGC
Van91I 2580 CCATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3063 CCACTTGGTGG
XhoI 989 CTCGAG C^TCGAG
3148 CTCGAG
XmaCI 151 CCCGGG C^CCGGG
198 CCCGGG
XmaI 151 CCCGGG C^CCGGG
198 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PstI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII