##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AgeI AhdI Alw44I AlwNI AocI
ApaI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AvaI AvaII
AviII AvrII BamHI BbeI BbrPI
BcgI BcnI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsoBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO65I EcoRV FauI
FseI FspI HapII HincII HindII
HindIII HpaI HpaII KasI KspI
MamI MluI MroI MspI MvnI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 501 GGTACC G^GTACC
AciI 534 CCGC CCGC(-3/-1)
AcyI 179 GGCGTC G[GA]^CG[CT]C
AflII 1715 CTTAAG C^TTAAG
AlwI 680 GATCC GGATC(4/5)
Aor51HI 359 AGCGCT AGC^GCT
Asp718I 501 GGTACC G^GTACC
BalI 1196 TGGCCA TGG^CCA
BanI 501 GGTACC G^G[CT][GA]CC
BbvCI 342 CCTCAGC CCTCAGC(-5/-2)
BciVI 2073 GTATCC GTATCC(6/5)
BclI 1410 TGATCA T^GATCA
BfrI 1715 CTTAAG C^TTAAG
BsaHI 179 GGCGTC G[GA]^CG[CT]C
BsmBI 55 CGTCTC CGTCTC(1/5)
BsmFI 151 GTCCC GGGAC(10/14)
BspMI 474 GCAGGT ACCTGC(4/8)
BsrDI 1636 CATTGC GCAATG(2/0)
BstXI 745 CCAAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 679 AGATCC [GA]^GATC[CT]
DraII 295 AGGCCCC [GA]G^G[ACGT]CC[CT]
EaeI 1196 TGGCCA [CT]^GGCC[GA]
Eco47III 359 AGCGCT AGC^GCT
EcoO109I 295 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 850 GAATTC G^AATTC
EcoT14I 2475 CCTTGG C^C[AT][AT]GG
FbaI 1410 TGATCA T^GATCA
HaeII 359 AGCGCT [GA]GCGC^[CT]
HgaI 180 GCGTC GACGC(5/10)
Hin1I 179 GGCGTC G[GA]^CG[CT]C
KpnI 501 GGTACC GGTAC^C
MfeI 1229 CAATTG C^AATTG
MflI 679 AGATCC [GA]^GATC[CT]
MluNI 1196 TGGCCA TGG^CCA
MscI 1196 TGGCCA TGG^CCA
MunI 1229 CAATTG C^AATTG
NdeI 46 CATATG CA^TATG
PshAI 242 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 424 GAGCTC GAGCT^C
SapI 336 GAAGAGC GCTCTTC(1/4)
ScaI 1647 AGTACT AGT^ACT
SphI 16 GCATGC GCATG^C
StyI 2475 CCTTGG C^C[AT][AT]GG
SwaI 2101 ATTTAAAT ATTT^AAAT
XhoII 679 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AspHI 424 GAGCTC G[AT]GC[AT]^C
1154 GTGCTC
BanII 424 GAGCTC G[GA]GC[CT]^C
558 GGGCTC
BbsI 350 GAAGAC GAAGAC(2/6)
1071 GTCTTC
BpuAI 350 GAAGAC GAAGAC(2/6)
1071 GTCTTC
BsgI 550 CTGCAC GTGCAG(16/14)
1275 CTGCAC
BsiHKAI 424 GAGCTC G[AT]GC[AT]^C
1154 GTGCTC
Cac8I 16 GCATGC GC[ACGT]^[ACGT]GC
362 GCTGGC
CfoI 360 GCGC GCG^C
394 GCGC
EcoT22I 1324 ATGCAT ATGCA^T
2193 ATGCAT
HhaI 360 GCGC GCG^C
394 GCGC
HinP1I 360 GCGC G^CGC
394 GCGC
MaeII 1632 ACGT A^CGT
2363 ACGT
MslI 1775 CATAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2283 CATGTTCATG
MspA1I 345 CAGCTG C[AC]G^C[GT]G
1436 CAGCTG
NsiI 1324 ATGCAT ATGCA^T
2193 ATGCAT
PstI 381 CTGCAG CTGCA^G
472 CTGCAG
PvuII 345 CAGCTG CAG^CTG
1436 CAGCTG
SmlI 331 CTCAAG C^T[CT][GA]AG
1715 CTTAAG
Tsp45I 444 GTGAC ^GT[GC]AC
478 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AcsI AfaI AflIII AluI ApoI
BbvI BfaI BmyI BsaJI BsiYI
BslI BsmAI BsmI Bsp1286I BspLU11I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII DraI EarI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I