##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       AccIII      AclI        AflIII      Alw44I      
AocI        Aor51HI     ApaLI       AscI        Asp700I     
AspI        AvaI        AviII       AvrII       BalI        
BbvCI       BcgI        BclI        BcnI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BseAI       
BsiEI       BsiWI       BsmI        BsoBI       BspDI       
BspEI       BspHI       BspLU11I    BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DraIII      DsaI        EagI        EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRV       
FbaI        FseI        FspI        HgaI        KspI        
MamI        MluI        MluNI       MroI        MscI        
NaeI        NciI        NcoI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PmeI        Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SphI        Sse8387I    Tth111I     XbaI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AccI          627  GTCTAC               GT^[AC][GT]AC
AcyI          230  GGCGCC               G[GA]^CG[CT]C
AflII        3350  CTTAAG               C^TTAAG
AgeI          757  ACCGGT               A^CCGGT
AhdI         1056  GACAAAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         1171  GGGCCC               GGGCC^C
AspEI        1056  GACAAAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI        2181  GGATCC               G^GATCC
BanII        1171  GGGCCC               G[GA]GC[CT]^C
BbeI          230  GGCGCC               GGCGC^C
BbrPI           1  CACGTG               CAC^GTG
BciVI        2593  GTATCC               GTATCC(6/5)
BfrI         3350  CTTAAG               C^TTAAG
BglI          233  GCCAGCGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI         230  GGCGCC               G[GA]^CG[CT]C
BsaI          615  GAGACC               GGTCTC(1/5)
BsaWI         757  ACCGGT               [AT]^CCGG[AT]
BsmBI         153  GAGACG               CGTCTC(1/5)
Bsp1407I     3412  TGTACA               T^GTACA
BsrBI        1279  CCGCTC               CCGCTC(-3/-3)
BsrGI        3412  TGTACA               T^GTACA
EaeI          157  CGGCCA               [CT]^GGCC[GA]
Eam1105I     1056  GACAAAATGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         993  CCTGGGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1220  GAATTC               G^AATTC
EcoT22I      1630  ATGCAT               ATGCA^T
HaeII         230  GGCGCC               [GA]GCGC^[CT]
Hin1I         230  GGCGCC               G[GA]^CG[CT]C
HindIII      1740  AAGCTT               A^AGCTT
HpaI         1619  GTTAAC               GTT^AAC
KasI          230  GGCGCC               G^GCGCC
MfeI         2909  CAATTG               C^AATTG
MunI         2909  CAATTG               C^AATTG
NarI          230  GGCGCC               GG^CGCC
NdeI         1137  CATATG               CA^TATG
NsiI         1630  ATGCAT               ATGCA^T
PinAI         757  ACCGGT               A^CCGGT
PmaCI           1  CACGTG               CAC^GTG
PmlI            1  CACGTG               CAC^GTG
PpuMI          76  GGGTCCC              [GA]G^G[AT]CC[CT]
PshAI        2627  GACTTGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI          873  GAAGAGC              GCTCTTC(1/4)
ScaI         1469  AGTACT               AGT^ACT
SexAI        1657  ACCTGGT              A^CC[AT]GGT
SfoI          230  GGCGCC               GGC^GCC
SpeI         2482  ACTAGT               A^CTAGT
SspBI        3412  TGTACA               T^GTACA
StuI          106  AGGCCT               AGG^CCT
SwaI         2883  ATTTAAAT             ATTT^AAAT
TaqI          590  TCGA                 T^CGA
TthHB8I       590  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

Acc65I        207  GGTACC               G^GTACC
             1661  GGTACC              
AccII         194  CGCG                 CG^CG
              238  CGCG                
AseI          386  ATTAAT               AT^TAAT
             2941  ATTAAT              
AsnI          386  ATTAAT               AT^TAAT
             2941  ATTAAT              
Asp718I       207  GGTACC               G^GTACC
             1661  GGTACC              
BsaAI           1  CACGTG               [CT]AC^GT[GA]
              183  TACGTG              
BstUI         194  CGCG                 CG^CG
              238  CGCG                
BstYI        2033  AGATCC               [GA]^GATC[CT]
             2181  GGATCC              
DraII          76  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             1171  GGGCCCC             
DrdI          405  GACAACAGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2299  GACATCAGTGTC        
EcoO109I       76  GGGTCCC              [GA]G^G[ACGT]CC[CT]
             1171  GGGCCCC             
EcoT14I       250  CCAAGG               C^C[AT][AT]GG
             2145  CCAAGG              
KpnI          207  GGTACC               GGTAC^C
             1661  GGTACC              
MflI         2033  AGATCC               [GA]^GATC[CT]
             2181  GGATCC              
MvnI          194  CGCG                 CG^CG
              238  CGCG                
PshBI         386  ATTAAT               AT^TAAT
             2941  ATTAAT              
PstI            8  CTGCAG               CTGCA^G
              243  CTGCAG              
StyI          250  CCAAGG               C^C[AT][AT]GG
             2145  CCAAGG              
Tsp45I        136  GTCAC                ^GT[GC]AC
              665  GTCAC               
XcmI           19  CCAGAGCAGCAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              704  CCAGCCCTTTGATGG     
XhoII        2033  AGATCC               [GA]^GATC[CT]
             2181  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AspHI       AvaII       BanI        
BbsI        BbvI        BfaI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrDI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       Cac8I       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraI        EarI        
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       Van91I