##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccI AclI AgeI AhdI
Aor51HI ApaI AscI AspEI BamHI
BciVI BclI BglI BglII BsiWI
BsmBI BspDI BssHII BssSI Bst1107I
BstXI BstZ17I ClaI CpoI DraIII
Eam1105I Eco47III EcoT22I FbaI FseI
KspI MfeI MluI MunI NaeI
NcoI NdeI NgoMIV NotI NruI
NsiI PacI PaeR7I PinAI PmeI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SgrAI SmaI SnaBI
SpeI Sse8387I SspI StuI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 1606 GGTACC G^GTACC
AccII 2332 CGCG CG^CG
AcyI 898 GGCGCC G[GA]^CG[CT]C
AflII 2986 CTTAAG C^TTAAG
Asp718I 1606 GGTACC G^GTACC
AvaI 1633 CCCGAG C^[CT]CG[GA]G
AviII 2326 TGCGCA TGC^GCA
AvrII 2767 CCTAGG C^CTAGG
BbeI 898 GGCGCC GGCGC^C
BbrPI 2634 CACGTG CAC^GTG
BbvCI 2738 CCTCAGC CCTCAGC(-5/-2)
BcnI 2774 CCGGG CC^[GC]GG
BfrI 2986 CTTAAG C^TTAAG
BlnI 2767 CCTAGG C^CTAGG
BlpI 3126 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3126 GCTCAGC GC^T[ACGT]AGC
BsaAI 2634 CACGTG [CT]AC^GT[GA]
BsaBI 4025 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 898 GGCGCC G[GA]^CG[CT]C
BsaI 2460 GAGACC GGTCTC(1/5)
BsiEI 1557 CGGCCG CG[GA][CT]^CG
BsoBI 1633 CCCGAG C^[CT]CG[GA]G
BspLU11I 3982 ACATGT A^CATGT
BsrBI 1447 GAGCGG CCGCTC(-3/-3)
BstEII 744 GGTGACC G^GT[ACGT]ACC
BstPI 744 GGTGACC G^GT[ACGT]ACC
BstUI 2332 CGCG CG^CG
CelII 3126 GCTCAGC GC^T[ACGT]AGC
DrdI 1701 GACTTCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1557 CGGCCG C^GGCCG
EclXI 1557 CGGCCG C^GGCCG
Eco52I 1557 CGGCCG C^GGCCG
EcoNI 2275 CCTGACTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 744 GGTGACC G^GT[ACGT]ACC
EcoRI 3833 GAATTC G^AATTC
EcoRV 1271 GATATC GAT^ATC
FauI 851 GCGGG CCCGC(4/6)
FspI 2326 TGCGCA TGC^GCA
HgaI 1764 GACGC GACGC(5/10)
Hin1I 898 GGCGCC G[GA]^CG[CT]C
HincII 2555 GTTAAC GT[CT]^[GA]AC
HindII 2555 GTTAAC GT[CT]^[GA]AC
HindIII 3736 AAGCTT A^AGCTT
HpaI 2555 GTTAAC GTT^AAC
KasI 898 GGCGCC G^GCGCC
KpnI 1606 GGTACC GGTAC^C
MamI 4025 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2332 CGCG CG^CG
NarI 898 GGCGCC GG^CGCC
NciI 2774 CCGGG CC^[GC]GG
NheI 2921 GCTAGC G^CTAGC
PflMI 2834 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2634 CACGTG CAC^GTG
PmlI 2634 CACGTG CAC^GTG
PshAI 121 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 2833 ACCAGGT A^CC[AT]GGT
SfoI 898 GGCGCC GGC^GCC
Van91I 2834 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2204 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Alw44I 1732 GTGCAC G^TGCAC
2319 GTGCAC
AlwNI 271 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
912 CAGCTGCTG
AocI 1464 CCTGAGG CC^T[ACGT]AGG
2663 CCTCAGG
ApaLI 1732 GTGCAC G^TGCAC
2319 GTGCAC
AseI 2387 ATTAAT AT^TAAT
4028 ATTAAT
AsnI 2387 ATTAAT AT^TAAT
4028 ATTAAT
Asp700I 1416 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1542 GAAAGCATTC
AspI 2772 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3227 GACTTTGTC
BalI 2091 TGGCCA TGG^CCA
3203 TGGCCA
BcgI 1724 CGATTTCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2693 CGATGGCAGTGC
Bsp1407I 7 TGTACA T^GTACA
3812 TGTACA
BspMI 1088 ACCTGC ACCTGC(4/8)
1580 GCAGGT
BsrGI 7 TGTACA T^GTACA
3812 TGTACA
Bsu36I 1464 CCTGAGG CC^T[ACGT]AGG
2663 CCTCAGG
DsaI 27 CCGTGG C^C[GA][CT]GG
3424 CCACGG
Eco81I 1464 CCTGAGG CC^T[ACGT]AGG
2663 CCTCAGG
HaeII 316 AGCGCC [GA]GCGC^[CT]
898 GGCGCC
MluNI 2091 TGGCCA TGG^CCA
3203 TGGCCA
MscI 2091 TGGCCA TGG^CCA
3203 TGGCCA
PflFI 2772 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3227 GACTTTGTC
PleI 267 GACTC GAGTC(4/5)
864 GACTC
PshBI 2387 ATTAAT AT^TAAT
4028 ATTAAT
SacI 3124 GAGCTC GAGCT^C
3452 GAGCTC
SapI 819 GAAGAGC GCTCTTC(1/4)
2847 GAAGAGC
ScaI 510 AGTACT AGT^ACT
3047 AGTACT
SspBI 7 TGTACA T^GTACA
3812 TGTACA
Tth111I 2772 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3227 GACTTTGTC
XcmI 18 CCAAACTTGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2535 CCATGCTCTCCTTGG
XmnI 1416 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1542 GAAAGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccIII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaII
BanI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspEI BspHI BsrDI BsrFI BsrI
BssKI BstAPI BstBI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MroI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI NspV PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfuI
SmlI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII