##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
Alw44I Aor51HI ApaLI AscI Asp718I
AviII AvrII BalI BbeI BbrPI
BcgI BclI BglII BlnI BsaAI
BseAI BsiWI BsmBI BspDI BspEI
BsrBI Bst1107I BstAPI BstZ17I ClaI
CpoI DraII DrdI DsaI EaeI
EagI EclXI Eco47III Eco52I EcoO109I
EcoRI EcoRV FbaI FseI FspI
HaeII HindIII KasI KpnI KspI
MluI MluNI MroI MscI NaeI
NarI NcoI NgoMIV NheI NotI
NruI PacI PaeR7I PmaCI PmeI
PmlI PpuMI Psp1406I PvuI RsrII
SacI SacII SalI ScaI SfiI
SfoI SgrAI SnaBI StuI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AcyI 863 GACGCC G[GA]^CG[CT]C
AgeI 1383 ACCGGT A^CCGGT
AhdI 625 GACGGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3316 CCTGAGG CC^T[ACGT]AGG
ApaI 1305 GGGCCC GGGCC^C
Asp700I 3670 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 625 GACGGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 454 GTGCTC G[AT]GC[AT]^C
AspI 892 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1458 CCCGGG C^[CT]CG[GA]G
BanI 1626 GGTGCC G^G[CT][GA]CC
BbvCI 1499 CCTCAGC CCTCAGC(-5/-2)
BciVI 3289 GGATAC GTATCC(6/5)
BsaBI 3290 GATACTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 863 GACGCC G[GA]^CG[CT]C
BsaI 3429 GGTCTC GGTCTC(1/5)
BsaWI 1383 ACCGGT [AT]^CCGG[AT]
BsiEI 769 CGGTCG CG[GA][CT]^CG
BsiHKAI 454 GTGCTC G[AT]GC[AT]^C
BsoBI 1458 CCCGGG C^[CT]CG[GA]G
Bsp1407I 3979 TGTACA T^GTACA
BsrGI 3979 TGTACA T^GTACA
BssHII 990 GCGCGC G^CGCGC
BssSI 886 CTCGTG CACGAG(-5/-1)
BstBI 360 TTCGAA TT^CGAA
Bsu36I 3316 CCTGAGG CC^T[ACGT]AGG
DraI 3692 TTTAAA TTT^AAA
Eam1105I 625 GACGGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3316 CCTGAGG CC^T[ACGT]AGG
EcoNI 1651 CCTTCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2922 ATGCAT ATGCA^T
FauI 867 CCCGC CCCGC(4/6)
HgaI 863 GACGC GACGC(5/10)
Hin1I 863 GACGCC G[GA]^CG[CT]C
HpaI 2016 GTTAAC GTT^AAC
MamI 3290 GATACTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 2518 CATATG CA^TATG
NsiI 2922 ATGCAT ATGCA^T
NspV 360 TTCGAA TT^CGAA
PflFI 892 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 844 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1383 ACCGGT A^CCGGT
PshAI 2811 GACTTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 420 CCTGCAGG CCTGCA^GG
SexAI 1967 ACCAGGT A^CC[AT]GGT
SfuI 360 TTCGAA TT^CGAA
SmaI 1458 CCCGGG CCC^GGG
SpeI 2788 ACTAGT A^CTAGT
SphI 1994 GCATGC GCATG^C
Sse8387I 420 CCTGCAGG CCTGCA^GG
SspBI 3979 TGTACA T^GTACA
SspI 4293 AATATT AAT^ATT
Tth111I 892 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 844 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2643 TCTAGA T^CTAGA
XmaCI 1458 CCCGGG C^CCGGG
XmaI 1458 CCCGGG C^CCGGG
XmnI 3670 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AseI 2081 ATTAAT AT^TAAT
3054 ATTAAT
AsnI 2081 ATTAAT AT^TAAT
3054 ATTAAT
BamHI 383 GGATCC G^GATCC
1926 GGATCC
BglI 483 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
666 GCCCACAGGGC
BlpI 14 GCTGAGC GC^T[ACGT]AGC
4138 GCTGAGC
BpmI 216 CTGGAG CTGGAG(16/14)
2133 CTGGAG
Bpu1102I 14 GCTGAGC GC^T[ACGT]AGC
4138 GCTGAGC
BseRI 823 CTCCTC GAGGAG(10/8)
2647 GAGGAG
BspHI 3138 TCATGA T^CATGA
3792 TCATGA
BsrDI 1851 CATTGC GCAATG(2/0)
4143 GCAATG
BstEII 1695 GGTGACC G^GT[ACGT]ACC
2214 GGTGACC
BstPI 1695 GGTGACC G^GT[ACGT]ACC
2214 GGTGACC
CelII 14 GCTGAGC GC^T[ACGT]AGC
4138 GCTGAGC
EcoO65I 1695 GGTGACC G^GT[ACGT]ACC
2214 GGTGACC
HincII 2016 GTTAAC GT[CT]^[GA]AC
3633 GTTGAC
HindII 2016 GTTAAC GT[CT]^[GA]AC
3633 GTTGAC
PshBI 2081 ATTAAT AT^TAAT
3054 ATTAAT
RcaI 3138 TCATGA T^CATGA
3792 TCATGA
SapI 1753 GAAGAGC GCTCTTC(1/4)
3890 GCTCTTC
XcmI 1041 CCAAAGCAGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1522 CCAGTGTAAGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI ApoI
AvaII BanII BbsI BbvI BcnI
BfaI BfrI BmyI BpuAI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspLU11I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraIII EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MfeI MflI
MnlI MseI MslI MspA1I MspI
MunI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII