##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AclI AgeI AhdI AocI
ApaI AscI Asp700I AspEI AspI
AviII AvrII BamHI BbeI BbvCI
BglI BglII BlnI BlpI Bpu1102I
BsiWI BsmBI BsmI Bsp1407I BspDI
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraIII Eam1105I Eco81I EcoNI
EcoRV FseI FspI KasI KspI
MfeI MluI MunI NarI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI Psp1406I PvuI RsrII
SacII SalI SfiI SfoI SfuI
SmaI SnaBI SpeI SspBI SwaI
Tth111I XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 4926 GGCGTC G[GA]^CG[CT]C
Alw44I 153 GTGCAC G^TGCAC
Aor51HI 2705 AGCGCT AGC^GCT
ApaLI 153 GTGCAC G^TGCAC
AvaI 2004 CCCGAG C^[CT]CG[GA]G
BbrPI 3549 CACGTG CAC^GTG
BcgI 3882 CGACCATGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 1077 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4926 GGCGTC G[GA]^CG[CT]C
BsgI 2610 GTGCAG GTGCAG(16/14)
BsoBI 2004 CCCGAG C^[CT]CG[GA]G
BsrDI 5049 GCAATG GCAATG(2/0)
BstAPI 1699 GCAAATTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2524 CCAGGACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 4087 GACAGTGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2705 AGCGCT AGC^GCT
EcoT22I 1203 ATGCAT ATGCA^T
FauI 139 CCCGC CCCGC(4/6)
HgaI 4927 GCGTC GACGC(5/10)
Hin1I 4926 GGCGTC G[GA]^CG[CT]C
HpaI 3124 GTTAAC GTT^AAC
MamI 1077 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 6 GCCGGC GCC^GGC
NdeI 282 CATATG CA^TATG
NgoMIV 6 GCCGGC G^CCGGC
NheI 15 GCTAGC G^CTAGC
NsiI 1203 ATGCAT ATGCA^T
PmaCI 3549 CACGTG CAC^GTG
PmlI 3549 CACGTG CAC^GTG
SacI 2007 GAGCTC GAGCT^C
SapI 1029 GCTCTTC GCTCTTC(1/4)
SexAI 2391 ACCTGGT A^CC[AT]GGT
SgrAI 5 CGCCGGCG C[GA]^CCGG[CT]G
SspI 3914 AATATT AAT^ATT
StuI 4959 AGGCCT AGG^CCT
XbaI 4684 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 364 GGTACC G^GTACC
1675 GGTACC
AccI 2378 GTCTAC GT^[AC][GT]AC
4529 GTAGAC
AccII 57 CGCG CG^CG
134 CGCG
AccIII 3082 TCCGGA T^CCGGA
3168 TCCGGA
AflII 1885 CTTAAG C^TTAAG
2491 CTTAAG
AseI 1807 ATTAAT AT^TAAT
3449 ATTAAT
AsnI 1807 ATTAAT AT^TAAT
3449 ATTAAT
Asp718I 364 GGTACC G^GTACC
1675 GGTACC
BanI 364 GGTACC G^G[CT][GA]CC
1675 GGTACC
BcnI 95 CCCGG CC^[GC]GG
1258 CCGGG
BfrI 1885 CTTAAG C^TTAAG
2491 CTTAAG
BsaAI 3549 CACGTG [CT]AC^GT[GA]
3599 CACGTA
BsaWI 3082 TCCGGA [AT]^CCGG[AT]
3168 TCCGGA
BseAI 3082 TCCGGA T^CCGGA
3168 TCCGGA
BsiEI 35 CGGCCG CG[GA][CT]^CG
97 CGGCCG
BspEI 3082 TCCGGA T^CCGGA
3168 TCCGGA
BspLU11I 2715 ACATGT A^CATGT
3494 ACATGT
BsrFI 6 GCCGGC [GA]^CCGG[CT]
1598 ACCGGC
BstEII 1275 GGTCACC G^GT[ACGT]ACC
2176 GGTTACC
BstPI 1275 GGTCACC G^GT[ACGT]ACC
2176 GGTTACC
BstUI 57 CGCG CG^CG
134 CGCG
Cfr10I 6 GCCGGC [GA]^CCGG[CT]
1598 ACCGGC
EagI 35 CGGCCG C^GGCCG
97 CGGCCG
EclXI 35 CGGCCG C^GGCCG
97 CGGCCG
Eco52I 35 CGGCCG C^GGCCG
97 CGGCCG
EcoO65I 1275 GGTCACC G^GT[ACGT]ACC
2176 GGTTACC
EcoRI 277 GAATTC G^AATTC
4284 GAATTC
HaeII 12 GGCGCT [GA]GCGC^[CT]
2705 AGCGCT
HindIII 162 AAGCTT A^AGCTT
2736 AAGCTT
KpnI 364 GGTACC GGTAC^C
1675 GGTACC
MroI 3082 TCCGGA T^CCGGA
3168 TCCGGA
MslI 225 CATGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1519 CATTGTTGTG
MvnI 57 CGCG CG^CG
134 CGCG
NciI 95 CCCGG CC^[GC]GG
1258 CCGGG
NcoI 1126 CCATGG C^CATGG
3387 CCATGG
PshAI 3408 GACGGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4508 GACCTCGGTC
PshBI 1807 ATTAAT AT^TAAT
3449 ATTAAT
SbfI 1406 CCTGCAGG CCTGCA^GG
4954 CCTGCAGG
ScaI 918 AGTACT AGT^ACT
4769 AGTACT
SphI 2022 GCATGC GCATG^C
3594 GCATGC
Sse8387I 1406 CCTGCAGG CCTGCA^GG
4954 CCTGCAGG
XcmI 2241 CCACACAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4107 CCACTTCTGGGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BanII BbsI BbvI BciVI
BclI BfaI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII