################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AclI AgeI AhdI AocI ApaI AscI Asp700I AspEI AspI AviII AvrII BamHI BbeI BbvCI BglI BglII BlnI BlpI Bpu1102I BsiWI BsmBI BsmI Bsp1407I BspDI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I CelII ClaI CpoI DraIII Eam1105I Eco81I EcoNI EcoRV FseI FspI KasI KspI MfeI MluI MunI NarI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI Psp1406I PvuI RsrII SacII SalI SfiI SfoI SfuI SmaI SnaBI SpeI SspBI SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AcyI 4926 GGCGTC G[GA]^CG[CT]C Alw44I 153 GTGCAC G^TGCAC Aor51HI 2705 AGCGCT AGC^GCT ApaLI 153 GTGCAC G^TGCAC AvaI 2004 CCCGAG C^[CT]CG[GA]G BbrPI 3549 CACGTG CAC^GTG BcgI 3882 CGACCATGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaBI 1077 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 4926 GGCGTC G[GA]^CG[CT]C BsgI 2610 GTGCAG GTGCAG(16/14) BsoBI 2004 CCCGAG C^[CT]CG[GA]G BsrDI 5049 GCAATG GCAATG(2/0) BstAPI 1699 GCAAATTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 2524 CCAGGACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 4087 GACAGTGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2705 AGCGCT AGC^GCT EcoT22I 1203 ATGCAT ATGCA^T FauI 139 CCCGC CCCGC(4/6) HgaI 4927 GCGTC GACGC(5/10) Hin1I 4926 GGCGTC G[GA]^CG[CT]C HpaI 3124 GTTAAC GTT^AAC MamI 1077 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 6 GCCGGC GCC^GGC NdeI 282 CATATG CA^TATG NgoMIV 6 GCCGGC G^CCGGC NheI 15 GCTAGC G^CTAGC NsiI 1203 ATGCAT ATGCA^T PmaCI 3549 CACGTG CAC^GTG PmlI 3549 CACGTG CAC^GTG SacI 2007 GAGCTC GAGCT^C SapI 1029 GCTCTTC GCTCTTC(1/4) SexAI 2391 ACCTGGT A^CC[AT]GGT SgrAI 5 CGCCGGCG C[GA]^CCGG[CT]G SspI 3914 AATATT AAT^ATT StuI 4959 AGGCCT AGG^CCT XbaI 4684 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 364 GGTACC G^GTACC 1675 GGTACC AccI 2378 GTCTAC GT^[AC][GT]AC 4529 GTAGAC AccII 57 CGCG CG^CG 134 CGCG AccIII 3082 TCCGGA T^CCGGA 3168 TCCGGA AflII 1885 CTTAAG C^TTAAG 2491 CTTAAG AseI 1807 ATTAAT AT^TAAT 3449 ATTAAT AsnI 1807 ATTAAT AT^TAAT 3449 ATTAAT Asp718I 364 GGTACC G^GTACC 1675 GGTACC BanI 364 GGTACC G^G[CT][GA]CC 1675 GGTACC BcnI 95 CCCGG CC^[GC]GG 1258 CCGGG BfrI 1885 CTTAAG C^TTAAG 2491 CTTAAG BsaAI 3549 CACGTG [CT]AC^GT[GA] 3599 CACGTA BsaWI 3082 TCCGGA [AT]^CCGG[AT] 3168 TCCGGA BseAI 3082 TCCGGA T^CCGGA 3168 TCCGGA BsiEI 35 CGGCCG CG[GA][CT]^CG 97 CGGCCG BspEI 3082 TCCGGA T^CCGGA 3168 TCCGGA BspLU11I 2715 ACATGT A^CATGT 3494 ACATGT BsrFI 6 GCCGGC [GA]^CCGG[CT] 1598 ACCGGC BstEII 1275 GGTCACC G^GT[ACGT]ACC 2176 GGTTACC BstPI 1275 GGTCACC G^GT[ACGT]ACC 2176 GGTTACC BstUI 57 CGCG CG^CG 134 CGCG Cfr10I 6 GCCGGC [GA]^CCGG[CT] 1598 ACCGGC EagI 35 CGGCCG C^GGCCG 97 CGGCCG EclXI 35 CGGCCG C^GGCCG 97 CGGCCG Eco52I 35 CGGCCG C^GGCCG 97 CGGCCG EcoO65I 1275 GGTCACC G^GT[ACGT]ACC 2176 GGTTACC EcoRI 277 GAATTC G^AATTC 4284 GAATTC HaeII 12 GGCGCT [GA]GCGC^[CT] 2705 AGCGCT HindIII 162 AAGCTT A^AGCTT 2736 AAGCTT KpnI 364 GGTACC GGTAC^C 1675 GGTACC MroI 3082 TCCGGA T^CCGGA 3168 TCCGGA MslI 225 CATGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1519 CATTGTTGTG MvnI 57 CGCG CG^CG 134 CGCG NciI 95 CCCGG CC^[GC]GG 1258 CCGGG NcoI 1126 CCATGG C^CATGG 3387 CCATGG PshAI 3408 GACGGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4508 GACCTCGGTC PshBI 1807 ATTAAT AT^TAAT 3449 ATTAAT SbfI 1406 CCTGCAGG CCTGCA^GG 4954 CCTGCAGG ScaI 918 AGTACT AGT^ACT 4769 AGTACT SphI 2022 GCATGC GCATG^C 3594 GCATGC Sse8387I 1406 CCTGCAGG CCTGCA^GG 4954 CCTGCAGG XcmI 2241 CCACACAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4107 CCACTTCTGGGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaII BalI BanII BbsI BbvI BciVI BclI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII