##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccI AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I BamHI BbrPI BciVI BclI
BfrI BsaBI BsiEI BsiWI BspDI
BspHI BspLU11I Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FbaI FseI HpaI KpnI
KspI MamI MluI NcoI NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshBI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SfuI SnaBI SpeI SphI
Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 3791 GACGTC GACGT^C
AccIII 376 TCCGGA T^CCGGA
AflIII 1060 ACACGT A^C[GA][CT]GT
Asp700I 2147 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2728 TGCGCA TGC^GCA
AvrII 2924 CCTAGG C^CTAGG
BalI 4178 TGGCCA TGG^CCA
BbeI 847 GGCGCC GGCGC^C
BcgI 1079 CGAGGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1189 AGATCT A^GATCT
BlnI 2924 CCTAGG C^CTAGG
BseAI 376 TCCGGA T^CCGGA
BspEI 376 TCCGGA T^CCGGA
BsrDI 4330 CATTGC GCAATG(2/0)
DraI 3283 TTTAAA TTT^AAA
DrdI 3241 GACTGTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 2728 TGCGCA TGC^GCA
HindIII 1871 AAGCTT A^AGCTT
KasI 847 GGCGCC G^GCGCC
MfeI 4079 CAATTG C^AATTG
MluNI 4178 TGGCCA TGG^CCA
MroI 376 TCCGGA T^CCGGA
MscI 4178 TGGCCA TGG^CCA
MslI 1651 CACTGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 4079 CAATTG C^AATTG
NaeI 844 GCCGGC GCC^GGC
NarI 847 GGCGCC GG^CGCC
NdeI 3352 CATATG CA^TATG
NgoMIV 844 GCCGGC G^CCGGC
PaeR7I 366 CTCGAG C^TCGAG
PshAI 1043 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 3699 GCTCTTC GCTCTTC(1/4)
SexAI 1225 ACCAGGT A^CC[AT]GGT
SfiI 2246 GGCCCAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 847 GGCGCC GGC^GCC
SgrAI 843 CGCCGGCG C[GA]^CCGG[CT]G
SspI 2150 AATATT AAT^ATT
XhoI 366 CTCGAG C^TCGAG
XmnI 2147 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AclI 1172 AACGTT AA^CGTT
1758 AACGTT
AhdI 288 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1821 GACTCCCAGTC
AspEI 288 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1821 GACTCCCAGTC
AspI 1730 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2904 GACAGGGTC
BlpI 1179 GCTGAGC GC^T[ACGT]AGC
2571 GCTCAGC
Bpu1102I 1179 GCTGAGC GC^T[ACGT]AGC
2571 GCTCAGC
BsaAI 1061 CACGTA [CT]AC^GT[GA]
1716 TACGTG
BsmBI 1735 CGTCTC CGTCTC(1/5)
2461 CGTCTC
BsmI 819 GCATTC GAATGC(1/-1)
2061 GCATTC
BsrBI 477 GAGCGG CCGCTC(-3/-3)
1305 GAGCGG
BssHII 134 GCGCGC G^CGCGC
428 GCGCGC
BssSI 651 CACGAG CACGAG(-5/-1)
3482 CACGAG
CelII 1179 GCTGAGC GC^T[ACGT]AGC
2571 GCTCAGC
EaeI 223 TGGCCG [CT]^GGCC[GA]
4178 TGGCCA
Eam1105I 288 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1821 GACTCCCAGTC
EcoT22I 87 ATGCAT ATGCA^T
817 ATGCAT
HincII 1518 GTTGAC GT[CT]^[GA]AC
2207 GTTGAC
HindII 1518 GTTGAC GT[CT]^[GA]AC
2207 GTTGAC
NsiI 87 ATGCAT ATGCA^T
817 ATGCAT
NspI 1942 ACATGC [GA]CATG^[CT]
3506 GCATGT
PflFI 1730 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2904 GACAGGGTC
PflMI 851 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2667 CCACTGCGTGG
Psp1406I 1172 AACGTT AA^CGTT
1758 AACGTT
Tth111I 1730 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
2904 GACAGGGTC
Van91I 851 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2667 CCACTGCGTGG
XbaI 1601 TCTAGA T^CTAGA
2706 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrFI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI