##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI Aor51HI AscI AseI AsnI
Asp700I AvrII BamHI BbeI BbrPI
BbvCI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaWI BseAI BsiWI
BsmBI BspDI BspEI BssHII Bst1107I
BstBI BstZ17I CelII ClaI CpoI
DrdI Eco47III EcoRI EcoRV FseI
KasI MfeI MluI MroI MunI
NarI NcoI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RsrII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SspI SwaI Van91I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 1952 GGTACC G^GTACC
AcsI 4085 AAATTC [GA]^AATT[CT]
AcyI 134 GACGCC G[GA]^CG[CT]C
AhdI 2386 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 4085 AAATTC [GA]^AATT[CT]
Asp718I 1952 GGTACC G^GTACC
AspEI 2386 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 398 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2620 TGCGCA TGC^GCA
BanI 1952 GGTACC G^G[CT][GA]CC
BciVI 649 GGATAC GTATCC(6/5)
BclI 1434 TGATCA T^GATCA
BglII 2009 AGATCT A^GATCT
BsaBI 1862 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 134 GACGCC G[GA]^CG[CT]C
BsmI 3547 GAATGC GAATGC(1/-1)
Bsp1407I 3534 TGTACA T^GTACA
BspHI 1618 TCATGA T^CATGA
BspMI 1827 ACCTGC ACCTGC(4/8)
BsrBI 46 GAGCGG CCGCTC(-3/-3)
BsrGI 3534 TGTACA T^GTACA
BssSI 1203 CTCGTG CACGAG(-5/-1)
BstAPI 3561 GCACATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1808 GGTCACC G^GT[ACGT]ACC
BstPI 1808 GGTCACC G^GT[ACGT]ACC
DraI 2539 TTTAAA TTT^AAA
DraIII 9 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 49 CGGCCG C^GGCCG
Eam1105I 2386 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 49 CGGCCG C^GGCCG
Eco52I 49 CGGCCG C^GGCCG
EcoO65I 1808 GGTCACC G^GT[ACGT]ACC
FbaI 1434 TGATCA T^GATCA
FspI 2620 TGCGCA TGC^GCA
HaeII 384 AGCGCC [GA]GCGC^[CT]
Hin1I 134 GACGCC G[GA]^CG[CT]C
HindIII 2990 AAGCTT A^AGCTT
HpaI 3940 GTTAAC GTT^AAC
KpnI 1952 GGTACC GGTAC^C
MamI 1862 GATACCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 279 GCCGGC GCC^GGC
NdeI 1514 CATATG CA^TATG
NgoMIV 279 GCCGGC G^CCGGC
NheI 826 GCTAGC G^CTAGC
PflFI 398 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 937 CGATCG CGAT^CG
RcaI 1618 TCATGA T^CATGA
SacI 893 GAGCTC GAGCT^C
SapI 890 GAAGAGC GCTCTTC(1/4)
SexAI 3771 ACCTGGT A^CC[AT]GGT
SpeI 2790 ACTAGT A^CTAGT
SspBI 3534 TGTACA T^GTACA
StuI 3160 AGGCCT AGG^CCT
Tth111I 398 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1673 CCAGTGACTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 1485 ACATGT A^C[GA][CT]GT
2215 ACATGT
Alw44I 1758 GTGCAC G^TGCAC
3897 GTGCAC
AlwNI 396 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
3023 CAGAATCTG
AocI 521 CCTCAGG CC^T[ACGT]AGG
3096 CCTGAGG
ApaLI 1758 GTGCAC G^TGCAC
3897 GTGCAC
BbsI 179 GAAGAC GAAGAC(2/6)
404 GTCTTC
BcgI 371 GCATCTCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3236 GCAAAACCGTCG
BpuAI 179 GAAGAC GAAGAC(2/6)
404 GTCTTC
BsaI 4109 GAGACC GGTCTC(1/5)
4193 GGTCTC
BseRI 251 CTCCTC GAGGAG(10/8)
266 GAGGAG
BsiEI 49 CGGCCG CG[GA][CT]^CG
937 CGATCG
BspLU11I 1485 ACATGT A^CATGT
2215 ACATGT
BsrDI 1560 CATTGC GCAATG(2/0)
1734 CATTGC
BsrFI 279 GCCGGC [GA]^CCGG[CT]
462 ACCGGC
BstXI 1022 CCAATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2185 CCAGACTGATGG
Bsu36I 521 CCTCAGG CC^T[ACGT]AGG
3096 CCTGAGG
Cfr10I 279 GCCGGC [GA]^CCGG[CT]
462 ACCGGC
Eco81I 521 CCTCAGG CC^T[ACGT]AGG
3096 CCTGAGG
EcoNI 1057 CCTAAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3406 CCTAGTTGAGG
HgaI 134 GACGC GACGC(5/10)
239 GCGTC
KspI 342 CCGCGG CCGC^GG
507 CCGCGG
MaeII 663 ACGT A^CGT
1474 ACGT
MslI 3697 CACAGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4118 CACCAGAATG
PpuMI 2092 AGGACCT [GA]G^G[AT]CC[CT]
3351 GGGACCT
SacII 342 CCGCGG CCGC^GG
507 CCGCGG
SmaI 358 CCCGGG CCC^GGG
787 CCCGGG
TfiI 2275 GAATC G^A[AT]TC
3025 GAATC
XmaCI 358 CCCGGG C^CCGGG
787 CCCGGG
XmaI 358 CCCGGG C^CCGGG
787 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
ApaI AspHI AvaI AvaII BalI
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII