##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccI AclI AflIII
AgeI Aor51HI AscI Asp700I Asp718I
AviII AvrII BamHI BbrPI BciVI
BlnI BsaAI BsaBI BsiWI BsmI
BspDI BspLU11I BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV FseI FspI HpaI
KpnI KspI MamI MluI NaeI
NdeI NgoMIV NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI Psp1406I RsrII SacII SalI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 1273 TCCGGA T^CCGGA
AcyI 3092 GGCGCC G[GA]^CG[CT]C
AflII 3005 CTTAAG C^TTAAG
AseI 369 ATTAAT AT^TAAT
AsnI 369 ATTAAT AT^TAAT
BbeI 3092 GGCGCC GGCGC^C
BfrI 3005 CTTAAG C^TTAAG
BsaHI 3092 GGCGCC G[GA]^CG[CT]C
BseAI 1273 TCCGGA T^CCGGA
BsiEI 1392 CGATCG CG[GA][CT]^CG
BspEI 1273 TCCGGA T^CCGGA
BsrFI 2764 ACCGGC [GA]^CCGG[CT]
Cfr10I 2764 ACCGGC [GA]^CCGG[CT]
DraIII 4837 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 3639 ATGCAT ATGCA^T
Hin1I 3092 GGCGCC G[GA]^CG[CT]C
KasI 3092 GGCGCC G^GCGCC
MfeI 5800 CAATTG C^AATTG
MroI 1273 TCCGGA T^CCGGA
MunI 5800 CAATTG C^AATTG
NarI 3092 GGCGCC GG^CGCC
NheI 6095 GCTAGC G^CTAGC
NsiI 3639 ATGCAT ATGCA^T
PaeR7I 1006 CTCGAG C^TCGAG
PshBI 369 ATTAAT AT^TAAT
PvuI 1392 CGATCG CGAT^CG
SbfI 3180 CCTGCAGG CCTGCA^GG
SfoI 3092 GGCGCC GGC^GCC
SmaI 3154 CCCGGG CCC^GGG
Sse8387I 3180 CCTGCAGG CCTGCA^GG
SspI 5268 AATATT AAT^ATT
XbaI 4226 TCTAGA T^CTAGA
XhoI 1006 CTCGAG C^TCGAG
XmaCI 3154 CCCGGG C^CCGGG
XmaI 3154 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 1704 CGCG CG^CG
2702 CGCG
AhdI 2439 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3480 GACAAAGGGTC
Alw44I 700 GTGCAC G^TGCAC
1878 GTGCAC
ApaLI 700 GTGCAC G^TGCAC
1878 GTGCAC
AspEI 2439 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3480 GACAAAGGGTC
AspI 207 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
4917 GACATGGTC
AvaI 1006 CTCGAG C^[CT]CG[GA]G
3154 CCCGGG
BalI 5673 TGGCCA TGG^CCA
6140 TGGCCA
BglI 1914 GCCAGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5082 GCCCACCCGGC
BsaWI 845 ACCGGA [AT]^CCGG[AT]
1273 TCCGGA
BsoBI 1006 CTCGAG C^[CT]CG[GA]G
3154 CCCGGG
Bsp1407I 163 TGTACA T^GTACA
3546 TGTACA
BspHI 221 TCATGA T^CATGA
6277 TCATGA
BsrGI 163 TGTACA T^GTACA
3546 TGTACA
BstUI 1704 CGCG CG^CG
2702 CGCG
DrdI 995 GACCTTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3120 GACAGCACCGTC
Eam1105I 2439 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3480 GACAAAGGGTC
EcoNI 3083 CCTCTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5066 CCTGCGCCAGG
MluNI 5673 TGGCCA TGG^CCA
6140 TGGCCA
MscI 5673 TGGCCA TGG^CCA
6140 TGGCCA
MvnI 1704 CGCG CG^CG
2702 CGCG
PflFI 207 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
4917 GACATGGTC
PflMI 252 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3373 CCACCCACTGG
RcaI 221 TCATGA T^CATGA
6277 TCATGA
SapI 1191 GAAGAGC GCTCTTC(1/4)
1752 GAAGAGC
SspBI 163 TGTACA T^GTACA
3546 TGTACA
Tth111I 207 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
4917 GACATGGTC
Van91I 252 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3373 CCACCCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaII BanI BanII BbsI BbvCI
BbvI BcgI BclI BcnI BfaI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BspMI BsrBI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRI EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII