##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccI AcyI AflIII AhdI
AscI AseI AsnI AspEI AviII
AvrII BbeI BciVI BclI BfaI
BlnI BsaBI BsaHI BsiWI BsmBI
Bsp1407I BspDI BspLU11I BsrGI Bst1107I
BstBI BstZ17I ClaI CpoI DrdI
Eam1105I EcoRI EcoRV EcoT22I FbaI
FseI FspI Hin1I HindIII HpaI
KasI KspI MaeI MamI MluI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PmeI PshAI
PshBI PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 644 GGTACC G^GTACC
AccIII 770 TCCGGA T^CCGGA
AclI 56 AACGTT AA^CGTT
AflII 2659 CTTAAG C^TTAAG
Alw44I 2104 GTGCAC G^TGCAC
AocI 2700 CCTGAGG CC^T[ACGT]AGG
ApaLI 2104 GTGCAC G^TGCAC
Asp718I 644 GGTACC G^GTACC
AspI 1135 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 2107 CACGTG CAC^GTG
BbsI 2319 GAAGAC GAAGAC(2/6)
BbvCI 151 GCTGAGG CCTCAGC(-5/-2)
BcgI 543 CGAGCAGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2659 CTTAAG C^TTAAG
BlpI 25 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 25 GCTCAGC GC^T[ACGT]AGC
BpuAI 2319 GAAGAC GAAGAC(2/6)
BsaAI 2107 CACGTG [CT]AC^GT[GA]
BseAI 770 TCCGGA T^CCGGA
BsiEI 451 CGGCCG CG[GA][CT]^CG
BspEI 770 TCCGGA T^CCGGA
BspHI 202 TCATGA T^CATGA
BssHII 2946 GCGCGC G^CGCGC
BssSI 2865 CACGAG CACGAG(-5/-1)
BstAPI 1604 GCAAGCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1083 GGTCACC G^GT[ACGT]ACC
BstPI 1083 GGTCACC G^GT[ACGT]ACC
Bsu36I 2700 CCTGAGG CC^T[ACGT]AGG
CelII 25 GCTCAGC GC^T[ACGT]AGC
DraI 3548 TTTAAA TTT^AAA
DraIII 1838 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 451 CGGCCG C^GGCCG
EclXI 451 CGGCCG C^GGCCG
Eco52I 451 CGGCCG C^GGCCG
Eco81I 2700 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1083 GGTCACC G^GT[ACGT]ACC
HgaI 3303 GACGC GACGC(5/10)
HincII 2182 GTCAAC GT[CT]^[GA]AC
HindII 2182 GTCAAC GT[CT]^[GA]AC
KpnI 644 GGTACC GGTAC^C
MfeI 3047 CAATTG C^AATTG
MroI 770 TCCGGA T^CCGGA
MunI 3047 CAATTG C^AATTG
PflFI 1135 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2107 CACGTG CAC^GTG
PmlI 2107 CACGTG CAC^GTG
Psp1406I 56 AACGTT AA^CGTT
RcaI 202 TCATGA T^CATGA
SexAI 664 ACCAGGT A^CC[AT]GGT
Tth111I 1135 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 117 GAATTT [GA]^AATT[CT]
2433 GAATTT
AgeI 1099 ACCGGT A^CCGGT
1463 ACCGGT
Aor51HI 1223 AGCGCT AGC^GCT
1607 AGCGCT
ApoI 117 GAATTT [GA]^AATT[CT]
2433 GAATTT
BglII 2132 AGATCT A^GATCT
3009 AGATCT
BsrDI 746 CATTGC GCAATG(2/0)
2012 GCAATG
BstXI 890 CCATTTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2803 CCATGAATCTGG
Eco47III 1223 AGCGCT AGC^GCT
1607 AGCGCT
EcoNI 1635 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2696 CCTCCCTGAGG
HaeII 1223 AGCGCT [GA]GCGC^[CT]
1607 AGCGCT
PaeR7I 2896 CTCGAG C^TCGAG
2903 CTCGAG
PinAI 1099 ACCGGT A^CCGGT
1463 ACCGGT
SmaI 403 CCCGGG CCC^GGG
1857 CCCGGG
SphI 549 GCATGC GCATG^C
2060 GCATGC
Tsp45I 1084 GTCAC ^GT[GC]AC
3199 GTCAC
XhoI 2896 CTCGAG C^TCGAG
2903 CTCGAG
XmaCI 403 CCCGGG C^CCGGG
1857 CCCGGG
XmaI 403 CCCGGG C^CCGGG
1857 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI Asp700I AspHI AvaI
AvaII BalI BamHI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI