##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AclI AflII AgeI Alw44I Aor51HI
ApaLI AscI AviII BbeI BbrPI
BcgI BfrI BsiEI BsiWI BsmBI
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Cfr10I CpoI DrdI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRI FseI FspI KasI KspI
MluI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 2731 GACGTC GACGT^C
Acc65I 1326 GGTACC G^GTACC
AccII 3482 CGCG CG^CG
AccIII 387 TCCGGA T^CCGGA
AflIII 3072 ACATGT A^C[GA][CT]GT
ApaI 899 GGGCCC GGGCC^C
Asp718I 1326 GGTACC G^GTACC
AspI 2857 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 379 CCTAGG C^CTAGG
BamHI 201 GGATCC G^GATCC
BclI 4353 TGATCA T^GATCA
BlnI 379 CCTAGG C^CTAGG
BsaBI 1656 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 387 TCCGGA T^CCGGA
BspDI 1286 ATCGAT AT^CGAT
BspEI 387 TCCGGA T^CCGGA
BspLU11I 3072 ACATGT A^CATGT
BsrBI 1924 GAGCGG CCGCTC(-3/-3)
BsrDI 2027 CATTGC GCAATG(2/0)
BssSI 4051 CACGAG CACGAG(-5/-1)
BstAPI 2169 GCAGTTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 3482 CGCG CG^CG
CfoI 3128 GCGC GCG^C
ClaI 1286 ATCGAT AT^CGAT
DraIII 3163 CACGTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 676 GATATC GAT^ATC
FbaI 4353 TGATCA T^GATCA
HaeII 3127 AGCGCC [GA]GCGC^[CT]
HhaI 3128 GCGC GCG^C
HinP1I 3128 GCGC G^CGC
HpaI 4505 GTTAAC GTT^AAC
KpnI 1326 GGTACC GGTAC^C
MamI 1656 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1559 CAATTG C^AATTG
MroI 387 TCCGGA T^CCGGA
MunI 1559 CAATTG C^AATTG
MvnI 3482 CGCG CG^CG
NdeI 3667 CATATG CA^TATG
PaeR7I 4461 CTCGAG C^TCGAG
PflFI 2857 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3449 GGGTCCC [GA]G^G[AT]CC[CT]
SexAI 3580 ACCTGGT A^CC[AT]GGT
SfiI 18 GGCCCGACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 99 CCCGGG CCC^GGG
SphI 4154 GCATGC GCATG^C
SspI 4664 AATATT AAT^ATT
StuI 2433 AGGCCT AGG^CCT
Tth111I 2857 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4721 TCTAGA T^CTAGA
XcmI 2099 CCATGGGGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4461 CTCGAG C^TCGAG
XmaCI 99 CCCGGG C^CCGGG
XmaI 99 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 3294 GTAGAC GT^[AC][GT]AC
3576 GTCTAC
AcyI 917 GGCGTC G[GA]^CG[CT]C
2731 GACGTC
AhdI 51 GACATGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1750 GACTTGCTGTC
AocI 2898 CCTGAGG CC^T[ACGT]AGG
3491 CCTGAGG
AseI 3532 ATTAAT AT^TAAT
4692 ATTAAT
AsnI 3532 ATTAAT AT^TAAT
4692 ATTAAT
Asp700I 742 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3240 GAAAAACTTC
AspEI 51 GACATGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1750 GACTTGCTGTC
AspHI 2067 GAGCAC G[AT]GC[AT]^C
2924 GTGCTC
BbvCI 4361 CCTCAGC CCTCAGC(-5/-2)
1030 GCTGAGG
BciVI 1714 GTATCC GTATCC(6/5)
2194 GTATCC
BsaAI 1393 TACGTG [CT]AC^GT[GA]
3163 CACGTA
BsaHI 917 GGCGTC G[GA]^CG[CT]C
2731 GACGTC
BsaWI 387 TCCGGA [AT]^CCGG[AT]
2214 TCCGGT
BsgI 867 CTGCAC GTGCAG(16/14)
3427 GTGCAG
BsiHKAI 2067 GAGCAC G[AT]GC[AT]^C
2924 GTGCTC
BspHI 2361 TCATGA T^CATGA
3236 TCATGA
Bsu36I 2898 CCTGAGG CC^T[ACGT]AGG
3491 CCTGAGG
DraI 159 TTTAAA TTT^AAA
4579 TTTAAA
Eam1105I 51 GACATGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1750 GACTTGCTGTC
Eco81I 2898 CCTGAGG CC^T[ACGT]AGG
3491 CCTGAGG
EcoNI 2894 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4326 CCTTGATGAGG
EcoT22I 1368 ATGCAT ATGCA^T
4152 ATGCAT
HgaI 2085 GACGC GACGC(5/10)
918 GCGTC
Hin1I 917 GGCGTC G[GA]^CG[CT]C
2731 GACGTC
HindIII 2644 AAGCTT A^AGCTT
3196 AAGCTT
NcoI 2099 CCATGG C^CATGG
2262 CCATGG
NsiI 1368 ATGCAT ATGCA^T
4152 ATGCAT
PshBI 3532 ATTAAT AT^TAAT
4692 ATTAAT
RcaI 2361 TCATGA T^CATGA
3236 TCATGA
SapI 2341 GAAGAGC GCTCTTC(1/4)
3220 GAAGAGC
XmnI 742 GAAGAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3240 GAAAAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrGI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CelII Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII