##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I ApaLI AscI Asp718I AspHI
AviII AvrII BamHI BbsI BciVI
BclI BfrI BlnI BpuAI BsaBI
BsiHKAI BsiWI BspDI BspHI BstBI
ClaI CpoI EcoNI EcoRV FbaI
FseI FspI HindIII HpaI KpnI
KspI MamI MluI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SexAI
SfuI SgrAI SmaI SnaBI SpeI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AhdI 3436 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1109 GGATC GGATC(4/5)
Aor51HI 1175 AGCGCT AGC^GCT
AseI 4106 ATTAAT AT^TAAT
AsnI 4106 ATTAAT AT^TAAT
Asp700I 3985 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3436 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2155 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 339 CCCGAG C^[CT]CG[GA]G
BbvCI 2766 GCTGAGG CCTCAGC(-5/-2)
BcgI 341 CGAGCCTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 229 AGATCT A^GATCT
BlpI 1866 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1866 GCTCAGC GC^T[ACGT]AGC
BsaI 2892 GAGACC GGTCTC(1/5)
BsiEI 1510 CGGCCG CG[GA][CT]^CG
BsmI 2706 GAATGC GAATGC(1/-1)
BsoBI 339 CCCGAG C^[CT]CG[GA]G
Bsp1407I 2180 TGTACA T^GTACA
BspLU11I 3536 ACATGT A^CATGT
BsrGI 2180 TGTACA T^GTACA
BssHII 50 GCGCGC G^CGCGC
BstAPI 3179 GCACAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 229 AGATCT [GA]^GATC[CT]
CelII 1866 GCTCAGC GC^T[ACGT]AGC
DraIII 846 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1510 CGGCCG C^GGCCG
Eam1105I 3436 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1510 CGGCCG C^GGCCG
Eco47III 1175 AGCGCT AGC^GCT
Eco52I 1510 CGGCCG C^GGCCG
EcoRI 1150 GAATTC G^AATTC
EcoT22I 3186 ATGCAT ATGCA^T
MfeI 4112 CAATTG C^AATTG
MflI 229 AGATCT [GA]^GATC[CT]
MunI 4112 CAATTG C^AATTG
NaeI 1384 GCCGGC GCC^GGC
NcoI 1208 CCATGG C^CATGG
NdeI 1331 CATATG CA^TATG
NgoMIV 1384 GCCGGC G^CCGGC
NsiI 3186 ATGCAT ATGCA^T
PflFI 2155 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1660 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4106 ATTAAT AT^TAAT
SbfI 2190 CCTGCAGG CCTGCA^GG
SfiI 1297 GGCCAGTTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 2492 GCATGC GCATG^C
Sse8387I 2190 CCTGCAGG CCTGCA^GG
SspBI 2180 TGTACA T^GTACA
SspI 1031 AATATT AAT^ATT
Tth111I 2155 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 229 AGATCT [GA]^GATC[CT]
XmnI 3985 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 83 TCCGGA T^CCGGA
142 TCCGGA
AlwNI 3088 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
4330 CAGAAACTG
BanI 58 GGCGCC G^G[CT][GA]CC
2541 GGCGCC
BbeI 58 GGCGCC GGCGC^C
2541 GGCGCC
BbrPI 2484 CACGTG CAC^GTG
2505 CACGTG
BseAI 83 TCCGGA T^CCGGA
142 TCCGGA
BspEI 83 TCCGGA T^CCGGA
142 TCCGGA
BsrBI 1941 CCGCTC CCGCTC(-3/-3)
1507 GAGCGG
BsrDI 1443 CATTGC GCAATG(2/0)
2997 GCAATG
Bst1107I 1267 GTATAC GTA^TAC
4070 GTATAC
BstEII 1285 GGTTACC G^GT[ACGT]ACC
2278 GGTCACC
BstPI 1285 GGTTACC G^GT[ACGT]ACC
2278 GGTCACC
BstZ17I 1267 GTATAC GTA^TAC
4070 GTATAC
EcoO65I 1285 GGTTACC G^GT[ACGT]ACC
2278 GGTCACC
KasI 58 GGCGCC G^GCGCC
2541 GGCGCC
MroI 83 TCCGGA T^CCGGA
142 TCCGGA
NarI 58 GGCGCC GG^CGCC
2541 GGCGCC
PflMI 489 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3846 CCAGCAGTTGG
PmaCI 2484 CACGTG CAC^GTG
2505 CACGTG
PmlI 2484 CACGTG CAC^GTG
2505 CACGTG
ScaI 1814 AGTACT AGT^ACT
2669 AGTACT
SfoI 58 GGCGCC GGC^GCC
2541 GGCGCC
Van91I 489 CCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3846 CCAGCAGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AocI ApaI
ApoI AvaII BalI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstXI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DrdI DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI