##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

AccI        AccIII      AflII       AgeI        AhdI        
AseI        AsnI        AspEI       BbeI        BbrPI       
BcgI        BclI        BfrI        BsaAI       BseAI       
BsiWI       BsmI        BspDI       BspEI       Bst1107I    
BstBI       BstZ17I     ClaI        CpoI        DraI        
DrdI        Eam1105I    EcoRI       EcoRV       FbaI        
HindIII     HpaI        KasI        MfeI        MluI        
MroI        MunI        NarI        NdeI        NruI        
NspV        PacI        PinAI       PmaCI       PmeI        
PmlI        PshBI       PvuI        RsrII       SalI        
ScaI        SexAI       SfoI        SfuI        SgrAI       
SnaBI       SpeI        SphI        SwaI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

Acc65I        557  GGTACC               G^GTACC
AclI         4939  AACGTT               AA^CGTT
AcsI         1324  AAATTT               [GA]^AATT[CT]
AflIII       1346  ACATGT               A^C[GA][CT]GT
ApoI         1324  AAATTT               [GA]^AATT[CT]
AscI         2071  GGCGCGCC             GG^CGCGCC
Asp718I       557  GGTACC               G^GTACC
AspI          147  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII         939  CCTAGG               C^CTAGG
BciVI        1534  GTATCC               GTATCC(6/5)
BglII        1409  AGATCT               A^GATCT
BlnI          939  CCTAGG               C^CTAGG
BsaWI        1702  TCCGGT               [AT]^CCGG[AT]
BspLU11I     1346  ACATGT               A^CATGT
DraIII       2153  CACCCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I      3103  ATGCAT               ATGCA^T
HincII       4430  GTCAAC               GT[CT]^[GA]AC
HindII       4430  GTCAAC               GT[CT]^[GA]AC
KpnI          557  GGTACC               GGTAC^C
NheI         4616  GCTAGC               G^CTAGC
NotI         1148  GCGGCCGC             GC^GGCCGC
NsiI         3103  ATGCAT               ATGCA^T
PflFI         147  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        2303  GACAGATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     4939  AACGTT               AA^CGTT
SapI         3917  GCTCTTC              GCTCTTC(1/4)
SbfI          927  CCTGCAGG             CCTGCA^GG
SfiI          879  GGCCACCAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I      927  CCTGCAGG             CCTGCA^GG
SspI         5068  AATATT               AAT^ATT
StuI         4626  AGGCCT               AGG^CCT
Tth111I       147  GACGACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         4297  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AocI         1563  CCTGAGG              CC^T[ACGT]AGG
             2526  CCTGAGG             
Aor51HI       957  AGCGCT               AGC^GCT
             1394  AGCGCT              
Asp700I       795  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1359  GAACCAGTTC          
AviII         431  TGCGCA               TGC^GCA
             3167  TGCGCA              
BamHI         990  GGATCC               G^GATCC
             1559  GGATCC              
BbvCI          72  CCTCAGC              CCTCAGC(-5/-2)
             3350  CCTCAGC             
BsaBI         609  GATCCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4135  GATTTTTATC          
BsmBI        1730  CGTCTC               CGTCTC(1/5)
             2830  GAGACG              
BspHI        1355  TCATGA               T^CATGA
             3071  TCATGA              
BsrDI        3039  CATTGC               GCAATG(2/0)
             3899  CATTGC              
BssHII        622  GCGCGC               G^CGCGC
             2072  GCGCGC              
BssSI          28  CACGAG               CACGAG(-5/-1)
             1122  CTCGTG              
BstAPI        768  GCACATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1488  GCACTCACTGC         
Bsu36I       1563  CCTGAGG              CC^T[ACGT]AGG
             2526  CCTGAGG             
Eco47III      957  AGCGCT               AGC^GCT
             1394  AGCGCT              
Eco81I       1563  CCTGAGG              CC^T[ACGT]AGG
             2526  CCTGAGG             
FspI          431  TGCGCA               TGC^GCA
             3167  TGCGCA              
KspI         2261  CCGCGG               CCGC^GG
             2421  CCGCGG              
MamI          609  GATCCGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             4135  GATTTTTATC          
NcoI          650  CCATGG               C^CATGG
             1868  CCATGG              
NspI         1346  ACATGT               [GA]CATG^[CT]
             1388  ACATGC              
PaeR7I        715  CTCGAG               C^TCGAG
             1806  CTCGAG              
RcaI         1355  TCATGA               T^CATGA
             3071  TCATGA              
SacII        2261  CCGCGG               CCGC^GG
             2421  CCGCGG              
XhoI          715  CTCGAG               C^TCGAG
             1806  CTCGAG              
XmnI          795  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1359  GAACCAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  140 enzymes) #
#########################################

AatII       AccII       AciI        AcyI        AfaI        
AluI        Alw44I      AlwI        AlwNI       ApaI        
ApaLI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
Bsp1407I    BspMI       BsrBI       BsrFI       BsrGI       
BsrI        BssKI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EagI        
EarI        EclXI       Eco52I      EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        FseI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        SmlI        
SspBI       StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        XhoII       XmaCI       XmaI