##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccII AccIII AclI AgeI AhdI
AscI AspEI AvrII BamHI BbeI
BbrPI BglII BlnI BlpI Bpu1102I
BseAI BsiWI BspDI BspEI BssHII
Bst1107I BstBI BstUI BstZ17I CelII
ClaI CpoI DrdI Eam1105I EcoRI
EcoRV HindIII HpaI KasI KspI
MluI MroI MvnI NarI NdeI
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SbfI ScaI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 285 GTCGAC GT^[AC][GT]AC
AflII 476 CTTAAG C^TTAAG
AocI 2872 CCTCAGG CC^T[ACGT]AGG
AseI 648 ATTAAT AT^TAAT
AsnI 648 ATTAAT AT^TAAT
Asp700I 2609 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1756 TGCGCA TGC^GCA
BbvCI 2304 CCTCAGC CCTCAGC(-5/-2)
BclI 869 TGATCA T^GATCA
BfrI 476 CTTAAG C^TTAAG
BsaAI 333 TACGTG [CT]AC^GT[GA]
BsaBI 4007 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4149 TCCGGT [AT]^CCGG[AT]
BsmI 3687 GCATTC GAATGC(1/-1)
Bsp1407I 4 TGTACA T^GTACA
BsrGI 4 TGTACA T^GTACA
BssSI 3317 CTCGTG CACGAG(-5/-1)
Bsu36I 2872 CCTCAGG CC^T[ACGT]AGG
Eco81I 2872 CCTCAGG CC^T[ACGT]AGG
EcoNI 2317 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 869 TGATCA T^GATCA
FspI 1756 TGCGCA TGC^GCA
MamI 4007 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 406 CAATTG C^AATTG
MunI 406 CAATTG C^AATTG
PflMI 1245 CCATCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 3246 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 648 ATTAAT AT^TAAT
SacI 3567 GAGCTC GAGCT^C
SalI 285 GTCGAC G^TCGAC
SapI 3162 GAAGAGC GCTCTTC(1/4)
SexAI 2986 ACCTGGT A^CC[AT]GGT
SfiI 2903 GGCCTCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 979 GCATGC GCATG^C
SspBI 4 TGTACA T^GTACA
StuI 2329 AGGCCT AGG^CCT
Van91I 1245 CCATCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 425 TCTAGA T^CTAGA
XmnI 2609 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AatII 1590 GACGTC GACGT^C
3940 GACGTC
Acc65I 241 GGTACC G^GTACC
2990 GGTACC
AlwI 13 GATCC GGATC(4/5)
1572 GGATC
Aor51HI 226 AGCGCT AGC^GCT
2527 AGCGCT
ApaI 2370 GGGCCC GGGCC^C
3517 GGGCCC
Asp718I 241 GGTACC G^GTACC
2990 GGTACC
BcgI 2836 CGAGGTCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1831 GCACTACTTTCG
BciVI 899 GTATCC GTATCC(6/5)
1711 GTATCC
BglI 2904 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3099 GCCTGCGTGGC
BsmBI 1733 CGTCTC CGTCTC(1/5)
4100 CGTCTC
BspHI 530 TCATGA T^CATGA
719 TCATGA
BspMI 1194 ACCTGC ACCTGC(4/8)
2354 ACCTGC
BsrBI 847 CCGCTC CCGCTC(-3/-3)
3076 CCGCTC
BstYI 12 AGATCC [GA]^GATC[CT]
1572 GGATCT
DraI 990 TTTAAA TTT^AAA
3835 TTTAAA
DraIII 938 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
3262 CACACAGTG
Eco47III 226 AGCGCT AGC^GCT
2527 AGCGCT
EcoT22I 2505 ATGCAT ATGCA^T
3766 ATGCAT
FseI 2862 GGCCGGCC GGCCGG^CC
2866 GGCCGGCC
HincII 285 GTCGAC GT[CT]^[GA]AC
1326 GTTGAC
HindII 285 GTCGAC GT[CT]^[GA]AC
1326 GTTGAC
KpnI 241 GGTACC GGTAC^C
2990 GGTACC
MflI 12 AGATCC [GA]^GATC[CT]
1572 GGATCT
NaeI 2863 GCCGGC GCC^GGC
2867 GCCGGC
NcoI 308 CCATGG C^CATGG
1148 CCATGG
NgoMIV 2863 GCCGGC G^CCGGC
2867 GCCGGC
NsiI 2505 ATGCAT ATGCA^T
3766 ATGCAT
PaeR7I 2768 CTCGAG C^TCGAG
3523 CTCGAG
RcaI 530 TCATGA T^CATGA
719 TCATGA
SmaI 1315 CCCGGG CCC^GGG
1937 CCCGGG
XhoI 2768 CTCGAG C^TCGAG
3523 CTCGAG
XhoII 12 AGATCC [GA]^GATC[CT]
1572 GGATCT
XmaCI 1315 CCCGGG C^CCGGG
1937 CCCGGG
XmaI 1315 CCCGGG C^CCGGG
1937 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI Alw44I AlwNI ApaLI ApoI
AspHI AspI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspLU11I BsrDI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EarI EclXI
Eco52I EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI