##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI ApoI AscI Asp718I AspI
AviII BcgI BciVI BseAI BsiWI
BspDI BspEI BspHI BspLU11I BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HpaI KpnI
KspI MfeI MroI MunI NaeI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PmeI
PshAI Psp1406I PvuI RcaI SacII
SalI SapI SbfI SfuI SpeI
Sse8387I SspI SwaI TaqI Tth111I
TthHB8I XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AflII 3247 CTTAAG C^TTAAG
AgeI 670 ACCGGT A^CCGGT
Alw44I 942 GTGCAC G^TGCAC
ApaLI 942 GTGCAC G^TGCAC
AseI 2199 ATTAAT AT^TAAT
AsnI 2199 ATTAAT AT^TAAT
AvrII 3122 CCTAGG C^CTAGG
BamHI 2744 GGATCC G^GATCC
BbeI 2239 GGCGCC GGCGC^C
BbrPI 2268 CACGTG CAC^GTG
BclI 2687 TGATCA T^GATCA
BfrI 3247 CTTAAG C^TTAAG
BlnI 3122 CCTAGG C^CTAGG
BsaBI 4008 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 670 ACCGGT [AT]^CCGG[AT]
BsiEI 43 CGGCCG CG[GA][CT]^CG
BsmBI 1371 CGTCTC CGTCTC(1/5)
CpoI 4363 CGGTCCG CG^G[AT]CCG
EagI 43 CGGCCG C^GGCCG
EclXI 43 CGGCCG C^GGCCG
Eco52I 43 CGGCCG C^GGCCG
FbaI 2687 TGATCA T^GATCA
HincII 2912 GTTGAC GT[CT]^[GA]AC
HindII 2912 GTTGAC GT[CT]^[GA]AC
HindIII 2612 AAGCTT A^AGCTT
KasI 2239 GGCGCC G^GCGCC
MamI 4008 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1006 ACGCGT A^CGCGT
NarI 2239 GGCGCC GG^CGCC
NheI 2108 GCTAGC G^CTAGC
PinAI 670 ACCGGT A^CCGGT
PmaCI 2268 CACGTG CAC^GTG
PmlI 2268 CACGTG CAC^GTG
PshBI 2199 ATTAAT AT^TAAT
RsrII 4363 CGGTCCG CG^G[AT]CCG
SacI 734 GAGCTC GAGCT^C
ScaI 1135 AGTACT AGT^ACT
SexAI 4195 ACCTGGT A^CC[AT]GGT
SfiI 116 GGCCCGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2239 GGCGCC GGC^GCC
SgrAI 4301 CGCCGGTG C[GA]^CCGG[CT]G
SnaBI 4042 TACGTA TAC^GTA
XbaI 1944 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 1006 ACGCGT A^C[GA][CT]GT
1567 ACGTGT
AhdI 1353 GACCGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3461 GACATAGGGTC
Aor51HI 469 AGCGCT AGC^GCT
4393 AGCGCT
Asp700I 500 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1648 GAACCCCTTC
AspEI 1353 GACCGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3461 GACATAGGGTC
BalI 219 TGGCCA TGG^CCA
3064 TGGCCA
BbsI 2450 GTCTTC GAAGAC(2/6)
3368 GTCTTC
BpuAI 2450 GTCTTC GAAGAC(2/6)
3368 GTCTTC
BsmI 344 GAATGC GAATGC(1/-1)
3544 GCATTC
Bsp1407I 4119 TGTACA T^GTACA
4384 TGTACA
BsrGI 4119 TGTACA T^GTACA
4384 TGTACA
BstAPI 264 GCATCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1324 GCAAGGAGTGC
DraIII 435 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
437 CACTGTGTG
DrdI 363 GACCGGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1851 GACTGGTGTGTC
Eam1105I 1353 GACCGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3461 GACATAGGGTC
EarI 2028 CTCTTC CTCTTC(1/4)
1880 GAAGAG
Eco47III 469 AGCGCT AGC^GCT
4393 AGCGCT
EcoNI 1870 CCTTAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2568 CCTTTGTGAGG
Ksp632I 2028 CTCTTC CTCTTC(1/4)
1880 GAAGAG
MluNI 219 TGGCCA TGG^CCA
3064 TGGCCA
MscI 219 TGGCCA TGG^CCA
3064 TGGCCA
NcoI 319 CCATGG C^CATGG
3400 CCATGG
SphI 883 GCATGC GCATG^C
1087 GCATGC
SspBI 4119 TGTACA T^GTACA
4384 TGTACA
XcmI 794 CCAGATGCTAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2253 CCAGGCACCTGTTGG
XmnI 500 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1648 GAACCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BanI BanII BbvCI
BbvI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII
XmaCI XmaI