##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AgeI AhdI AscI AspEI
BcgI BciVI BglII BsiWI Bsp1407I
BspDI BspHI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
DraI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FseI
HincII HindII HpaI MfeI MunI
NcoI NdeI NotI NruI NsiI
NspV PaeR7I PinAI PmeI PshAI
PvuI RcaI SalI SbfI ScaI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 1965 GGTACC G^GTACC
AccI 4140 GTAGAC GT^[AC][GT]AC
AccIII 242 TCCGGA T^CCGGA
AclI 2706 AACGTT AA^CGTT
AcsI 2728 AAATTC [GA]^AATT[CT]
AflII 3489 CTTAAG C^TTAAG
Alw44I 3671 GTGCAC G^TGCAC
Aor51HI 3485 AGCGCT AGC^GCT
ApaI 3081 GGGCCC GGGCC^C
ApaLI 3671 GTGCAC G^TGCAC
ApoI 2728 AAATTC [GA]^AATT[CT]
AseI 3781 ATTAAT AT^TAAT
AsnI 3781 ATTAAT AT^TAAT
Asp700I 1755 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1965 GGTACC G^GTACC
AspI 2786 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2140 CCCGAG C^[CT]CG[GA]G
AvrII 3300 CCTAGG C^CTAGG
BamHI 3973 GGATCC G^GATCC
BbeI 3296 GGCGCC GGCGC^C
BbrPI 3117 CACGTG CAC^GTG
BclI 2944 TGATCA T^GATCA
BfrI 3489 CTTAAG C^TTAAG
BglI 2337 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3300 CCTAGG C^CTAGG
BlpI 3241 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3241 GCTCAGC GC^T[ACGT]AGC
BseAI 242 TCCGGA T^CCGGA
BsiEI 2313 CGGTCG CG[GA][CT]^CG
BsmBI 2374 CGTCTC CGTCTC(1/5)
BsmI 2063 GAATGC GAATGC(1/-1)
BsoBI 2140 CCCGAG C^[CT]CG[GA]G
BspEI 242 TCCGGA T^CCGGA
BspLU11I 4136 ACATGT A^CATGT
BssSI 2441 CACGAG CACGAG(-5/-1)
CelII 3241 GCTCAGC GC^T[ACGT]AGC
CpoI 2603 CGGACCG CG^G[AT]CCG
Eco47III 3485 AGCGCT AGC^GCT
FbaI 2944 TGATCA T^GATCA
HindIII 210 AAGCTT A^AGCTT
KasI 3296 GGCGCC G^GCGCC
KpnI 1965 GGTACC GGTAC^C
MluI 2290 ACGCGT A^CGCGT
MroI 242 TCCGGA T^CCGGA
NarI 3296 GGCGCC GG^CGCC
PacI 3778 TTAATTAA TTAAT^TAA
PflFI 2786 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3117 CACGTG CAC^GTG
PmlI 3117 CACGTG CAC^GTG
PshBI 3781 ATTAAT AT^TAAT
Psp1406I 2706 AACGTT AA^CGTT
RsrII 2603 CGGACCG CG^G[AT]CCG
SapI 1434 GAAGAGC GCTCTTC(1/4)
SfoI 3296 GGCGCC GGC^GCC
Tth111I 2786 GACGGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1484 CCAGGAGCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1755 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 2290 ACGCGT A^C[GA][CT]GT
4136 ACATGT
AviII 2715 TGCGCA TGC^GCA
2841 TGCGCA
BalI 122 TGGCCA TGG^CCA
1496 TGGCCA
BbsI 1035 GAAGAC GAAGAC(2/6)
2929 GTCTTC
BbvCI 435 GCTGAGG CCTCAGC(-5/-2)
2569 GCTGAGG
BpuAI 1035 GAAGAC GAAGAC(2/6)
2929 GTCTTC
BsaAI 3017 TACGTG [CT]AC^GT[GA]
3117 CACGTG
BsaBI 3968 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3989 GATTCAAATC
BstAPI 2287 GCAACGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3673 GCACACATTGC
DrdI 3262 GACCGGACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3885 GACTCCAGAGTC
FspI 2715 TGCGCA TGC^GCA
2841 TGCGCA
KspI 60 CCGCGG CCGC^GG
2210 CCGCGG
MamI 3968 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3989 GATTCAAATC
MluNI 122 TGGCCA TGG^CCA
1496 TGGCCA
MscI 122 TGGCCA TGG^CCA
1496 TGGCCA
MslI 2269 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3685 CACCTGGGTG
NaeI 156 GCCGGC GCC^GGC
2369 GCCGGC
NgoMIV 156 GCCGGC G^CCGGC
2369 GCCGGC
NheI 2596 GCTAGC G^CTAGC
3072 GCTAGC
SacI 2576 GAGCTC GAGCT^C
3947 GAGCTC
SacII 60 CCGCGG CCGC^GG
2210 CCGCGG
SphI 2283 GCATGC GCATG^C
2838 GCATGC
TaqI 215 TCGA T^CGA
2414 TCGA
TthHB8I 215 TCGA T^CGA
2414 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XhoII