##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AflII AgeI Alw44I ApaI
ApaLI AscI AseI AsnI Asp700I
AviII BbrPI BcgI BfrI BsaBI
BsiWI BspDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HindIII
KspI MamI MluI NdeI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshBI PvuI
RsrII SacII SbfI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 3843 GGTACC G^GTACC
AccIII 343 TCCGGA T^CCGGA
AclI 4037 AACGTT AA^CGTT
AcsI 2290 GAATTT [GA]^AATT[CT]
Aor51HI 3918 AGCGCT AGC^GCT
ApoI 2290 GAATTT [GA]^AATT[CT]
Asp718I 3843 GGTACC G^GTACC
AspI 1293 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 346 GGATAC GTATCC(6/5)
BclI 4921 TGATCA T^GATCA
BsaAI 4888 TACGTG [CT]AC^GT[GA]
BseAI 343 TCCGGA T^CCGGA
BspEI 343 TCCGGA T^CCGGA
BspHI 2597 TCATGA T^CATGA
EagI 1413 CGGCCG C^GGCCG
EclXI 1413 CGGCCG C^GGCCG
Eco47III 3918 AGCGCT AGC^GCT
Eco52I 1413 CGGCCG C^GGCCG
FbaI 4921 TGATCA T^GATCA
KpnI 3843 GGTACC GGTAC^C
MfeI 907 CAATTG C^AATTG
MroI 343 TCCGGA T^CCGGA
MunI 907 CAATTG C^AATTG
NaeI 1449 GCCGGC GCC^GGC
NgoMIV 1449 GCCGGC G^CCGGC
NheI 4795 GCTAGC G^CTAGC
PflFI 1293 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 804 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4037 AACGTT AA^CGTT
RcaI 2597 TCATGA T^CATGA
SalI 811 GTCGAC G^TCGAC
SmaI 4247 CCCGGG CCC^GGG
Tth111I 1293 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 4247 CCCGGG C^CCGGG
XmaI 4247 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 811 GTCGAC GT^[AC][GT]AC
5008 GTCTAC
AocI 2352 CCTGAGG CC^T[ACGT]AGG
3520 CCTCAGG
AvrII 4005 CCTAGG C^CTAGG
4784 CCTAGG
BbeI 29 GGCGCC GGCGC^C
4625 GGCGCC
BbvCI 1137 CCTCAGC CCTCAGC(-5/-2)
3921 GCTGAGG
BlnI 4005 CCTAGG C^CTAGG
4784 CCTAGG
BsaWI 343 TCCGGA [AT]^CCGG[AT]
4077 ACCGGA
BsiEI 89 CGGTCG CG[GA][CT]^CG
1413 CGGCCG
BsmBI 1823 GAGACG CGTCTC(1/5)
3294 GAGACG
Bsp1407I 1343 TGTACA T^GTACA
1699 TGTACA
BspLU11I 3155 ACATGT A^CATGT
4106 ACATGT
BspMI 2114 GCAGGT ACCTGC(4/8)
4668 GCAGGT
BsrGI 1343 TGTACA T^GTACA
1699 TGTACA
BstAPI 1246 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4204 GCAAGGCCTGC
BstXI 1220 CCAGCACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2200 CCAGCAGGCTGG
Bsu36I 2352 CCTGAGG CC^T[ACGT]AGG
3520 CCTCAGG
DraIII 3051 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
4258 CACACTGTG
Eco81I 2352 CCTGAGG CC^T[ACGT]AGG
3520 CCTCAGG
HpaI 912 GTTAAC GTT^AAC
2637 GTTAAC
KasI 29 GGCGCC G^GCGCC
4625 GGCGCC
NarI 29 GGCGCC GG^CGCC
4625 GGCGCC
PaeR7I 1264 CTCGAG C^TCGAG
2169 CTCGAG
SapI 4113 GAAGAGC GCTCTTC(1/4)
1063 GCTCTTC
SexAI 3229 ACCTGGT A^CC[AT]GGT
3725 ACCTGGT
SfoI 29 GGCGCC GGC^GCC
4625 GGCGCC
SspBI 1343 TGTACA T^GTACA
1699 TGTACA
XhoI 1264 CTCGAG C^TCGAG
2169 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AhdI AluI AlwI AlwNI AspEI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvI BcnI
BfaI BglI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrBI BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
Eam1105I EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII