##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AccI AclI AflII AscI
AseI AsnI Asp700I Asp718I AviII
BamHI BcgI BciVI BfrI BsaBI
BsiWI BsmI Bsp1407I BspDI BspLU11I
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I ClaI
CpoI DraI EcoO65I EcoRV FspI
HindIII HpaI KpnI MamI NdeI
NruI NspV PacI PmeI PshBI
Psp1406I PvuI RsrII SalI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SspBI SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 1109 TCCGGA T^CCGGA
AgeI 1842 ACCGGT A^CCGGT
Alw44I 2340 GTGCAC G^TGCAC
AlwI 4010 GATCC GGATC(4/5)
ApaLI 2340 GTGCAC G^TGCAC
AvrII 1020 CCTAGG C^CTAGG
BbeI 4413 GGCGCC GGCGC^C
BbrPI 3461 CACGTG CAC^GTG
BbsI 3785 GAAGAC GAAGAC(2/6)
BclI 4357 TGATCA T^GATCA
BglII 4388 AGATCT A^GATCT
BlnI 1020 CCTAGG C^CTAGG
BpuAI 3785 GAAGAC GAAGAC(2/6)
BseAI 1109 TCCGGA T^CCGGA
BspEI 1109 TCCGGA T^CCGGA
BspHI 5011 TCATGA T^CATGA
BssSI 101 CACGAG CACGAG(-5/-1)
BstYI 4388 AGATCT [GA]^GATC[CT]
DrdI 5179 GACCATGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1375 GAATTC G^AATTC
EcoT22I 2447 ATGCAT ATGCA^T
FbaI 4357 TGATCA T^GATCA
HincII 4482 GTCAAC GT[CT]^[GA]AC
HindII 4482 GTCAAC GT[CT]^[GA]AC
KasI 4413 GGCGCC G^GCGCC
MfeI 1934 CAATTG C^AATTG
MflI 4388 AGATCT [GA]^GATC[CT]
MluI 84 ACGCGT A^CGCGT
MroI 1109 TCCGGA T^CCGGA
MseI 4474 TTAA T^TAA
MunI 1934 CAATTG C^AATTG
NarI 4413 GGCGCC GG^CGCC
NheI 1338 GCTAGC G^CTAGC
NotI 5507 GCGGCCGC GC^GGCCGC
NsiI 2447 ATGCAT ATGCA^T
NspI 2445 GCATGC [GA]CATG^[CT]
PinAI 1842 ACCGGT A^CCGGT
PmaCI 3461 CACGTG CAC^GTG
PmlI 3461 CACGTG CAC^GTG
PshAI 4176 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 5011 TCATGA T^CATGA
SacI 3066 GAGCTC GAGCT^C
SapI 2527 GCTCTTC GCTCTTC(1/4)
SbfI 1430 CCTGCAGG CCTGCA^GG
SfoI 4413 GGCGCC GGC^GCC
SphI 2445 GCATGC GCATG^C
Sse8387I 1430 CCTGCAGG CCTGCA^GG
Tru9I 4474 TTAA T^TAA
XhoII 4388 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AcsI 1375 GAATTC [GA]^AATT[CT]
4314 GAATTT
AhdI 1437 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4674 GACGCACTGTC
Aor51HI 710 AGCGCT AGC^GCT
2269 AGCGCT
ApoI 1375 GAATTC [GA]^AATT[CT]
4314 GAATTT
AspEI 1437 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4674 GACGCACTGTC
BsrDI 1967 CATTGC GCAATG(2/0)
2364 GCAATG
Eam1105I 1437 GACCTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4674 GACGCACTGTC
Eco47III 710 AGCGCT AGC^GCT
2269 AGCGCT
FseI 929 GGCCGGCC GGCCGG^CC
1455 GGCCGGCC
NcoI 1879 CCATGG C^CATGG
4612 CCATGG
SfiI 769 GGCCCAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3072 GGCCAACGTGGCC
StuI 1123 AGGCCT AGG^CCT
2613 AGGCCT
XcmI 289 CCATGCGGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3412 CCACGCACTGGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AflIII AluI AlwNI AocI ApaI
AspHI AspI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PaeR7I PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XhoI XmaCI
XmaI