##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccII AccIII AcyI AgeI
AlwNI AocI Aor51HI ApaI AscI
AvrII BbeI BbrPI BbvCI BcgI
BcnI BlnI BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BsrBI BssHII BstEII BstPI
BstUI BstXI Bsu36I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV FseI HgaI
Hin1I KasI KspI MamI MluI
MroI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PvuI RcaI RsrII SacII
SalI SbfI SfiI SfoI SgrAI
SmaI SphI Sse8387I StuI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 5173 GGTACC G^GTACC
AclI 4991 AACGTT AA^CGTT
AseI 1202 ATTAAT AT^TAAT
AsnI 1202 ATTAAT AT^TAAT
Asp718I 5173 GGTACC G^GTACC
AspI 724 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1833 TGCGCA TGC^GCA
BamHI 2575 GGATCC G^GATCC
BanI 5173 GGTACC G^G[CT][GA]CC
BanII 1756 GAGCTC G[GA]GC[CT]^C
BclI 4830 TGATCA T^GATCA
BsaWI 536 ACCGGA [AT]^CCGG[AT]
BsrFI 4093 ACCGGC [GA]^CCGG[CT]
BssSI 308 CACGAG CACGAG(-5/-1)
BstBI 5202 TTCGAA TT^CGAA
Cfr10I 4093 ACCGGC [GA]^CCGG[CT]
EcoRI 1696 GAATTC G^AATTC
EcoT22I 3286 ATGCAT ATGCA^T
FauI 277 CCCGC CCCGC(4/6)
FbaI 4830 TGATCA T^GATCA
FspI 1833 TGCGCA TGC^GCA
HaeII 4525 GGCGCT [GA]GCGC^[CT]
KpnI 5173 GGTACC GGTAC^C
NcoI 4015 CCATGG C^CATGG
NsiI 3286 ATGCAT ATGCA^T
NspV 5202 TTCGAA TT^CGAA
PflFI 724 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1202 ATTAAT AT^TAAT
Psp1406I 4991 AACGTT AA^CGTT
PvuII 4519 CAGCTG CAG^CTG
SacI 1756 GAGCTC GAGCT^C
SapI 3431 GCTCTTC GCTCTTC(1/4)
ScaI 1027 AGTACT AGT^ACT
SexAI 2901 ACCAGGT A^CC[AT]GGT
SfuI 5202 TTCGAA TT^CGAA
SpeI 847 ACTAGT A^CTAGT
Tth111I 724 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5004 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AccI 352 GTATAC GT^[AC][GT]AC
5899 GTATAC
AflII 4162 CTTAAG C^TTAAG
4485 CTTAAG
AhdI 611 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2773 GACTAGGTGTC
AspEI 611 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2773 GACTAGGTGTC
BciVI 1246 GTATCC GTATCC(6/5)
5933 GTATCC
BfrI 4162 CTTAAG C^TTAAG
4485 CTTAAG
BglI 2120 GCCAGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4453 GCCTCTTGGGC
BlpI 2621 GCTAAGC GC^T[ACGT]AGC
4322 GCTCAGC
Bpu1102I 2621 GCTAAGC GC^T[ACGT]AGC
4322 GCTCAGC
BsaAI 1323 TACGTA [CT]AC^GT[GA]
5186 TACGTA
BsaI 4155 GAGACC GGTCTC(1/5)
4215 GAGACC
Bsp1407I 3265 TGTACA T^GTACA
4896 TGTACA
BsrDI 7 CATTGC GCAATG(2/0)
2742 GCAATG
BsrGI 3265 TGTACA T^GTACA
4896 TGTACA
Bst1107I 352 GTATAC GTA^TAC
5899 GTATAC
BstAPI 1433 GCATCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2235 GCATACCTTGC
BstZ17I 352 GTATAC GTA^TAC
5899 GTATAC
Cac8I 1918 GCAGGC GC[ACGT]^[ACGT]GC
3679 GCTTGC
CelII 2621 GCTAAGC GC^T[ACGT]AGC
4322 GCTCAGC
CfoI 1834 GCGC GCG^C
4526 GCGC
DraII 273 AGGACCC [GA]G^G[ACGT]CC[CT]
5735 AGGACCT
DraIII 2934 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
5346 CACATAGTG
DsaI 581 CCACGG C^C[GA][CT]GG
4015 CCATGG
Eam1105I 611 GACCAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2773 GACTAGGTGTC
EcoNI 1539 CCTCTAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4607 CCTGTCTTAGG
EcoO109I 273 AGGACCC [GA]G^G[ACGT]CC[CT]
5735 AGGACCT
EcoT14I 3051 CCAAGG C^C[AT][AT]GG
4015 CCATGG
HapII 537 CCGG C^CGG
4094 CCGG
HhaI 1834 GCGC GCG^C
4526 GCGC
HinP1I 1834 GCGC G^CGC
4526 GCGC
HindIII 570 AAGCTT A^AGCTT
5518 AAGCTT
HpaI 5405 GTTAAC GTT^AAC
6185 GTTAAC
HpaII 537 CCGG C^CGG
4094 CCGG
MfeI 1215 CAATTG C^AATTG
1821 CAATTG
MspI 537 CCGG C^CGG
4094 CCGG
MunI 1215 CAATTG C^AATTG
1821 CAATTG
PflMI 2902 CCAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4752 CCAGTAGATGG
PpuMI 273 AGGACCC [GA]G^G[AT]CC[CT]
5735 AGGACCT
SnaBI 1323 TACGTA TAC^GTA
5186 TACGTA
SspBI 3265 TGTACA T^GTACA
4896 TGTACA
StyI 3051 CCAAGG C^C[AT][AT]GG
4015 CCATGG
Van91I 2902 CCAGGTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4752 CCAGTAGATGG
XcmI 2106 CCAAAAGCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2410 CCATAGAAAAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI ApaLI ApoI Asp700I
AspHI AvaI AvaII BalI BbsI
BbvI BfaI BglII BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspLU11I BspMI BsrI
BssKI BstF5I BstNI BstYI Cfr13I
DdeI DpnII DraI EaeI EarI
EcoRII Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MvaI MwoI NdeI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI