##################################
# NO CUT ENZYMES ( 40 enzymes) #
##################################
AccIII AclI AflII AgeI AscI
AseI AsnI AvrII BbeI BfrI
BlnI BseAI BsiWI BspEI BstBI
CpoI DraI FseI KasI MfeI
MluI MroI MunI NarI NdeI
NheI NruI NspV PinAI PmeI
PshAI PshBI Psp1406I RsrII SfoI
SfuI SgrAI SnaBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 23 enzymes) #
###################################
BbrPI 3170 CACGTG CAC^GTG
BciVI 8889 GGATAC GTATCC(6/5)
BssHII 9381 GCGCGC G^CGCGC
Bst1107I 8268 GTATAC GTA^TAC
BstZ17I 8268 GTATAC GTA^TAC
EcoRV 8227 GATATC GAT^ATC
EcoT22I 9552 ATGCAT ATGCA^T
HindIII 672 AAGCTT A^AGCTT
HpaI 12726 GTTAAC GTT^AAC
KspI 7324 CCGCGG CCGC^GG
NotI 8030 GCGGCCGC GC^GGCCGC
NsiI 9552 ATGCAT ATGCA^T
PacI 2197 TTAATTAA TTAAT^TAA
PaeR7I 1851 CTCGAG C^TCGAG
PmaCI 3170 CACGTG CAC^GTG
PmlI 3170 CACGTG CAC^GTG
PvuI 9871 CGATCG CGAT^CG
SacII 7324 CCGCGG CCGC^GG
SalI 3768 GTCGAC G^TCGAC
SfiI 4506 GGCCATCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 12330 ACTAGT A^CTAGT
XbaI 1551 TCTAGA T^CTAGA
XhoI 1851 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AatII 5898 GACGTC GACGT^C
12229 GACGTC
Acc65I 5 GGTACC G^GTACC
6831 GGTACC
AccI 3768 GTCGAC GT^[AC][GT]AC
8268 GTATAC
AocI 304 CCTCAGG CC^T[ACGT]AGG
7578 CCTGAGG
Asp718I 5 GGTACC G^GTACC
6831 GGTACC
BsaWI 4963 ACCGGA [AT]^CCGG[AT]
10947 ACCGGA
BsmBI 9664 GAGACG CGTCTC(1/5)
12142 GAGACG
BspLU11I 4379 ACATGT A^CATGT
6095 ACATGT
Bsu36I 304 CCTCAGG CC^T[ACGT]AGG
7578 CCTGAGG
EagI 4301 CGGCCG C^GGCCG
8031 CGGCCG
EclXI 4301 CGGCCG C^GGCCG
8031 CGGCCG
Eco52I 4301 CGGCCG C^GGCCG
8031 CGGCCG
Eco81I 304 CCTCAGG CC^T[ACGT]AGG
7578 CCTGAGG
KpnI 5 GGTACC GGTAC^C
6831 GGTACC
#########################################
# 3 OR MORE CUT ENZYMES ( 181 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AhdI AluI Alw44I AlwI
AlwNI Aor51HI ApaI ApaLI ApoI
Asp700I AspEI AspHI AspI AvaI
AvaII AviII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcgI
BclI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BsrI
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
ClaI DdeI DpnII DraII DraIII
DrdI DsaI EaeI Eam1105I EarI
Eco47III EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I SbfI ScaI
ScrFI SexAI SfaNI SfcI SmaI
SmlI SphI Sse8387I SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI
XmnI