##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AccIII AflII AgeI AocI AscI
AvrII BbvCI BcgI BciVI BfrI
BlnI BsaWI BseAI BsiWI BsmBI
BsmI BspDI BspEI BspHI BsrDI
Bst1107I BstBI BstXI BstZ17I Bsu36I
ClaI CpoI EagI EclXI Eco52I
Eco81I EcoRV FseI HindIII HpaI
MluI MroI NotI NruI NspV
PacI PaeR7I PinAI PmeI PvuI
RcaI RsrII SalI SbfI SfiI
SfuI SgrAI SnaBI Sse8387I SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 965 GACGTC GACGT^C
Acc65I 3466 GGTACC G^GTACC
AclI 2627 AACGTT AA^CGTT
Aor51HI 1033 AGCGCT AGC^GCT
Asp718I 3466 GGTACC G^GTACC
AviII 2549 TGCGCA TGC^GCA
BamHI 1884 GGATCC G^GATCC
BbrPI 262 CACGTG CAC^GTG
BclI 4452 TGATCA T^GATCA
BglI 662 GCCCTCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1177 AGATCT A^GATCT
BsaBI 4383 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 305 CTCCTC GAGGAG(10/8)
BsiEI 1019 CGACCG CG[GA][CT]^CG
Bsp1407I 3958 TGTACA T^GTACA
BsrGI 3958 TGTACA T^GTACA
BstAPI 539 GCAGCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2095 GGTCACC G^GT[ACGT]ACC
BstPI 2095 GGTCACC G^GT[ACGT]ACC
DrdI 4765 GACAAAATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1033 AGCGCT AGC^GCT
EcoO65I 2095 GGTCACC G^GT[ACGT]ACC
FbaI 4452 TGATCA T^GATCA
FspI 2549 TGCGCA TGC^GCA
KpnI 3466 GGTACC GGTAC^C
MamI 4383 GATAATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4873 CAATTG C^AATTG
MunI 4873 CAATTG C^AATTG
NheI 786 GCTAGC G^CTAGC
PmaCI 262 CACGTG CAC^GTG
PmlI 262 CACGTG CAC^GTG
PshAI 4076 GACGACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2627 AACGTT AA^CGTT
SexAI 2862 ACCTGGT A^CC[AT]GGT
SpeI 5514 ACTAGT A^CTAGT
SphI 4415 GCATGC GCATG^C
SspBI 3958 TGTACA T^GTACA
XcmI 772 CCACGCTGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AhdI 1086 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3701 GACCAAAAGTC
Alw44I 678 GTGCAC G^TGCAC
2131 GTGCAC
ApaI 815 GGGCCC GGGCC^C
5436 GGGCCC
ApaLI 678 GTGCAC G^TGCAC
2131 GTGCAC
AseI 2378 ATTAAT AT^TAAT
5609 ATTAAT
AsnI 2378 ATTAAT AT^TAAT
5609 ATTAAT
AspEI 1086 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3701 GACCAAAAGTC
AspI 1603 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2267 GACATAGTC
BalI 973 TGGCCA TGG^CCA
1559 TGGCCA
BlpI 141 GCTGAGC GC^T[ACGT]AGC
2809 GCTGAGC
Bpu1102I 141 GCTGAGC GC^T[ACGT]AGC
2809 GCTGAGC
BsrBI 60 CCGCTC CCGCTC(-3/-3)
330 CCGCTC
BsrFI 574 GCCGGC [GA]^CCGG[CT]
853 GCCGGC
BssHII 49 GCGCGC G^CGCGC
942 GCGCGC
BssSI 543 CTCGTG CACGAG(-5/-1)
615 CTCGTG
CelII 141 GCTGAGC GC^T[ACGT]AGC
2809 GCTGAGC
Cfr10I 574 GCCGGC [GA]^CCGG[CT]
853 GCCGGC
DraIII 1110 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
2134 CACGTCGTG
Eam1105I 1086 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3701 GACCAAAAGTC
KspI 319 CCGCGG CCGC^GG
884 CCGCGG
MluNI 973 TGGCCA TGG^CCA
1559 TGGCCA
MscI 973 TGGCCA TGG^CCA
1559 TGGCCA
NaeI 574 GCCGGC GCC^GGC
853 GCCGGC
NcoI 1799 CCATGG C^CATGG
4229 CCATGG
NdeI 3434 CATATG CA^TATG
4426 CATATG
NgoMIV 574 GCCGGC G^CCGGC
853 GCCGGC
PflFI 1603 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2267 GACATAGTC
PshBI 2378 ATTAAT AT^TAAT
5609 ATTAAT
PvuII 1971 CAGCTG CAG^CTG
3290 CAGCTG
SacI 612 GAGCTC GAGCT^C
1219 GAGCTC
SacII 319 CCGCGG CCGC^GG
884 CCGCGG
SapI 5918 GAAGAGC GCTCTTC(1/4)
332 GCTCTTC
ScaI 595 AGTACT AGT^ACT
2794 AGTACT
SmaI 460 CCCGGG CCC^GGG
650 CCCGGG
StuI 1078 AGGCCT AGG^CCT
4261 AGGCCT
Tth111I 1603 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2267 GACATAGTC
XmaCI 460 CCCGGG C^CCGGG
650 CCCGGG
XmaI 460 CCCGGG C^CCGGG
650 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI Asp700I AspHI AvaI AvaII
BanI BanII BbeI BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspLU11I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRI EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI