##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
AclI AflII AgeI Alw44I ApaLI
AscI AseI AsnI AvrII BciVI
BclI BfrI BglII BlnI BsaBI
BsiWI BsrDI BssHII Bst1107I BstBI
BstZ17I DraI EcoRI EcoRV FbaI
HindIII HpaI MamI MfeI MunI
NdeI NotI NruI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
SalI ScaI SexAI SfuI SnaBI
SpeI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
Asp700I 6360 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2048 TGCGCA TGC^GCA
BbrPI 1975 CACGTG CAC^GTG
BbvCI 770 CCTCAGC CCTCAGC(-5/-2)
Bsp1407I 6562 TGTACA T^GTACA
BspHI 5447 TCATGA T^CATGA
BspLU11I 1682 ACATGT A^CATGT
BsrGI 6562 TGTACA T^GTACA
BssSI 4617 CTCGTG CACGAG(-5/-1)
CpoI 647 CGGTCCG CG^G[AT]CCG
DraIII 5687 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
FspI 2048 TGCGCA TGC^GCA
KspI 864 CCGCGG CCGC^GG
MluI 4178 ACGCGT A^CGCGT
MseI 6608 TTAA T^TAA
PmaCI 1975 CACGTG CAC^GTG
PmlI 1975 CACGTG CAC^GTG
RcaI 5447 TCATGA T^CATGA
RsrII 647 CGGTCCG CG^G[AT]CCG
SacII 864 CCGCGG CCGC^GG
SbfI 409 CCTGCAGG CCTGCA^GG
SgrAI 596 CGCCGGCG C[GA]^CCGG[CT]G
SphI 4020 GCATGC GCATG^C
Sse8387I 409 CCTGCAGG CCTGCA^GG
SspBI 6562 TGTACA T^GTACA
Tru9I 6608 TTAA T^TAA
XmnI 6360 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 2602 GGTACC G^GTACC
4525 GGTACC
AccI 1702 GTCTAC GT^[AC][GT]AC
4425 GTCTAC
AccIII 1744 TCCGGA T^CCGGA
2898 TCCGGA
AhdI 1504 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6083 GACCTCGAGTC
Asp718I 2602 GGTACC G^GTACC
4525 GGTACC
AspEI 1504 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6083 GACCTCGAGTC
AspI 643 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2236 GACACGGTC
BalI 1278 TGGCCA TGG^CCA
1940 TGGCCA
BamHI 162 GGATCC G^GATCC
3779 GGATCC
BcgI 5022 CGACACCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2387 GCAGCACCTTCG
BsaAI 1975 CACGTG [CT]AC^GT[GA]
2248 TACGTG
BsaWI 1744 TCCGGA [AT]^CCGG[AT]
2898 TCCGGA
BseAI 1744 TCCGGA T^CCGGA
2898 TCCGGA
BsmI 655 GAATGC GAATGC(1/-1)
2419 GAATGC
BspDI 5131 ATCGAT AT^CGAT
6618 ATCGAT
BspEI 1744 TCCGGA T^CCGGA
2898 TCCGGA
ClaI 5131 ATCGAT AT^CGAT
6618 ATCGAT
Eam1105I 1504 GACACCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6083 GACCTCGAGTC
EcoT22I 2421 ATGCAT ATGCA^T
6614 ATGCAT
HincII 580 GTCAAC GT[CT]^[GA]AC
1876 GTCAAC
HindII 580 GTCAAC GT[CT]^[GA]AC
1876 GTCAAC
KpnI 2602 GGTACC GGTAC^C
4525 GGTACC
MluNI 1278 TGGCCA TGG^CCA
1940 TGGCCA
MroI 1744 TCCGGA T^CCGGA
2898 TCCGGA
MscI 1278 TGGCCA TGG^CCA
1940 TGGCCA
NheI 3037 GCTAGC G^CTAGC
3702 GCTAGC
NsiI 2421 ATGCAT ATGCA^T
6614 ATGCAT
PflFI 643 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2236 GACACGGTC
PshAI 4175 GACACGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5639 GACGGGAGTC
SapI 2770 GAAGAGC GCTCTTC(1/4)
1368 GCTCTTC
Tth111I 643 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2236 GACACGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI Aor51HI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DrdI DsaI EaeI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI FseI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PaeR7I
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SfoI SmaI
SmlI StuI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoI XhoII XmaCI
XmaI