##################################
# NO CUT ENZYMES (   59 enzymes) #
##################################

AatII       AflIII      AgeI        AhdI        Alw44I      
ApaLI       AscI        AseI        AsnI        AspEI       
AvrII       BbeI        BlnI        BsaBI       BsiWI       
BspDI       BspLU11I    BssSI       BstBI       ClaI        
CpoI        EagI        Eam1105I    EclXI       Eco52I      
EcoRV       FseI        HpaI        KasI        MamI        
MluI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshAI       PshBI       
PvuI        RsrII       SalI        SapI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SpeI        StuI        SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I       3378  GGTACC               G^GTACC
AccIII        862  TCCGGA               T^CCGGA
AclI         4201  AACGTT               AA^CGTT
Aor51HI      5898  AGCGCT               AGC^GCT
Asp718I      3378  GGTACC               G^GTACC
AspI         1166  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        6220  TGCGCA               TGC^GCA
BbrPI        2447  CACGTG               CAC^GTG
BcgI         5439  CGAGCAGACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        3660  GTATCC               GTATCC(6/5)
BclI         5043  TGATCA               T^GATCA
BsaWI         862  TCCGGA               [AT]^CCGG[AT]
BseAI         862  TCCGGA               T^CCGGA
BsiEI        1030  CGACCG               CG[GA][CT]^CG
Bsp1407I     4346  TGTACA               T^GTACA
BspEI         862  TCCGGA               T^CCGGA
BspHI        5754  TCATGA               T^CATGA
BsrGI        4346  TGTACA               T^GTACA
BssHII       3882  GCGCGC               G^CGCGC
Bst1107I     5629  GTATAC               GTA^TAC
BstAPI       4511  GCAGGGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I      5629  GTATAC               GTA^TAC
Eco47III     5898  AGCGCT               AGC^GCT
FbaI         5043  TGATCA               T^GATCA
FspI         6220  TGCGCA               TGC^GCA
HindIII      2282  AAGCTT               A^AGCTT
KpnI         3378  GGTACC               GGTAC^C
KspI          364  CCGCGG               CCGC^GG
MroI          862  TCCGGA               T^CCGGA
PflFI        1166  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI        2447  CACGTG               CAC^GTG
PmlI         2447  CACGTG               CAC^GTG
Psp1406I     4201  AACGTT               AA^CGTT
RcaI         5754  TCATGA               T^CATGA
SacI          590  GAGCTC               GAGCT^C
SacII         364  CCGCGG               CCGC^GG
SbfI         2956  CCTGCAGG             CCTGCA^GG
SnaBI        4089  TACGTA               TAC^GTA
SphI         5310  GCATGC               GCATG^C
Sse8387I     2956  CCTGCAGG             CCTGCA^GG
SspBI        4346  TGTACA               T^GTACA
SspI         5242  AATATT               AAT^ATT
Tth111I      1166  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3424  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcyI         1547  GGCGTC               G[GA]^CG[CT]C
             2717  GACGCC              
AflII        1145  CTTAAG               C^TTAAG
             4226  CTTAAG              
AocI         2548  CCTGAGG              CC^T[ACGT]AGG
             3076  CCTGAGG             
ApaI         3208  GGGCCC               GGGCC^C
             4709  GGGCCC              
AvaI          435  CCCGGG               C^[CT]CG[GA]G
             1896  CCCGGG              
BamHI         852  GGATCC               G^GATCC
              859  GGATCC              
BfrI         1145  CTTAAG               C^TTAAG
             4226  CTTAAG              
BglI         2877  GCCCCAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3228  GCCCAGAGGGC         
BsaAI        2447  CACGTG               [CT]AC^GT[GA]
             4089  TACGTA              
BsaHI        1547  GGCGTC               G[GA]^CG[CT]C
             2717  GACGCC              
BsmBI        2804  CGTCTC               CGTCTC(1/5)
             6251  GAGACG              
BsoBI         435  CCCGGG               C^[CT]CG[GA]G
             1896  CCCGGG              
Bsu36I       2548  CCTGAGG              CC^T[ACGT]AGG
             3076  CCTGAGG             
DraI         4034  TTTAAA               TTT^AAA
             6506  TTTAAA              
DrdI         1901  GACGGAGGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2966  GACACAGTGGTC        
Eco81I       2548  CCTGAGG              CC^T[ACGT]AGG
             3076  CCTGAGG             
EcoT22I      1003  ATGCAT               ATGCA^T
             6468  ATGCAT              
Hin1I        1547  GGCGTC               G[GA]^CG[CT]C
             2717  GACGCC              
HincII        914  GTCAAC               GT[CT]^[GA]AC
             3754  GTTGAC              
HindII        914  GTCAAC               GT[CT]^[GA]AC
             3754  GTTGAC              
MfeI         5234  CAATTG               C^AATTG
             6001  CAATTG              
MunI         5234  CAATTG               C^AATTG
             6001  CAATTG              
NsiI         1003  ATGCAT               ATGCA^T
             6468  ATGCAT              
SmaI          435  CCCGGG               CCC^GGG
             1896  CCCGGG              
TaqI         1956  TCGA                 T^CGA
             2153  TCGA                
TthHB8I      1956  TCGA                 T^CGA
             2153  TCGA                
XcmI          856  CCAGGATCCGGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             4732  CCAAAGCAGCCATGG     
XmaCI         435  CCCGGG               C^CCGGG
             1896  CCCGGG              
XmaI          435  CCCGGG               C^CCGGG
             1896  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  126 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AfaI        
AluI        AlwI        AlwNI       ApoI        Asp700I     
AspHI       AvaII       BalI        BanI        BanII       
BbsI        BbvCI       BbvI        BcnI        BfaI        
BglII       BlpI        BmyI        BpmI        Bpu1102I    
BpuAI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BspMI       BsrBI       BsrDI       
BsrFI       BsrI        BssKI       BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstYI       
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DraIII      DsaI        
EaeI        EarI        EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Van91I      XhoII       
XmnI