##################################
# NO CUT ENZYMES ( 41 enzymes) #
##################################
AgeI AseI AsnI AviII AvrII
BlnI BsiWI BspDI BsrFI Cfr10I
ClaI DraIII EagI EclXI Eco52I
EcoRV FseI FspI MfeI MluI
MunI NaeI NdeI NgoMIV NotI
PacI PaeR7I PinAI PmeI PshBI
PvuI SalI SapI SbfI ScaI
SfiI SgrAI SnaBI Sse8387I SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 4446 GGTACC G^GTACC
AclI 5489 AACGTT AA^CGTT
AflII 6132 CTTAAG C^TTAAG
AocI 4017 CCTCAGG CC^T[ACGT]AGG
Aor51HI 342 AGCGCT AGC^GCT
ApaI 984 GGGCCC GGGCC^C
AscI 136 GGCGCGCC GG^CGCGCC
Asp700I 5272 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4446 GGTACC G^GTACC
AspI 4070 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 3173 CACGTG CAC^GTG
BbvCI 2252 CCTCAGC CCTCAGC(-5/-2)
BciVI 3424 GGATAC GTATCC(6/5)
BclI 1773 TGATCA T^GATCA
BfrI 6132 CTTAAG C^TTAAG
BlpI 2618 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2618 GCTGAGC GC^T[ACGT]AGC
BsiEI 887 CGACCG CG[GA][CT]^CG
Bsp1407I 5482 TGTACA T^GTACA
BspLU11I 4752 ACATGT A^CATGT
BsrGI 5482 TGTACA T^GTACA
BssHII 137 GCGCGC G^CGCGC
BstBI 5361 TTCGAA TT^CGAA
Bsu36I 4017 CCTCAGG CC^T[ACGT]AGG
CelII 2618 GCTGAGC GC^T[ACGT]AGC
CpoI 93 CGGACCG CG^G[AT]CCG
Eco47III 342 AGCGCT AGC^GCT
Eco81I 4017 CCTCAGG CC^T[ACGT]AGG
FbaI 1773 TGATCA T^GATCA
HincII 5458 GTTAAC GT[CT]^[GA]AC
HindII 5458 GTTAAC GT[CT]^[GA]AC
HindIII 255 AAGCTT A^AGCTT
HpaI 5458 GTTAAC GTT^AAC
KpnI 4446 GGTACC GGTAC^C
NheI 3526 GCTAGC G^CTAGC
NruI 3282 TCGCGA TCG^CGA
NspV 5361 TTCGAA TT^CGAA
PflFI 4070 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3173 CACGTG CAC^GTG
PmlI 3173 CACGTG CAC^GTG
Psp1406I 5489 AACGTT AA^CGTT
RsrII 93 CGGACCG CG^G[AT]CCG
SfuI 5361 TTCGAA TT^CGAA
SpeI 1409 ACTAGT A^CTAGT
SspBI 5482 TGTACA T^GTACA
SspI 5543 AATATT AAT^ATT
Tth111I 4070 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 5272 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 2073 TCCGGA T^CCGGA
6100 TCCGGA
AhdI 2019 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6091 GACAAAGTGTC
AspEI 2019 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6091 GACAAAGTGTC
BbeI 75 GGCGCC GGCGC^C
1087 GGCGCC
BglII 3459 AGATCT A^GATCT
5238 AGATCT
BsaBI 1774 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2605 GATCGTGATC
BseAI 2073 TCCGGA T^CCGGA
6100 TCCGGA
BspEI 2073 TCCGGA T^CCGGA
6100 TCCGGA
BspHI 1755 TCATGA T^CATGA
5190 TCATGA
BssSI 333 CACGAG CACGAG(-5/-1)
2204 CTCGTG
Bst1107I 2691 GTATAC GTA^TAC
5351 GTATAC
BstEII 2581 GGTGACC G^GT[ACGT]ACC
4591 GGTGACC
BstPI 2581 GGTGACC G^GT[ACGT]ACC
4591 GGTGACC
BstZ17I 2691 GTATAC GTA^TAC
5351 GTATAC
DrdI 2305 GACGGGCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4649 GACATCGGCGTC
Eam1105I 2019 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6091 GACAAAGTGTC
EcoO65I 2581 GGTGACC G^GT[ACGT]ACC
4591 GGTGACC
KasI 75 GGCGCC G^GCGCC
1087 GGCGCC
KspI 80 CCGCGG CCGC^GG
3956 CCGCGG
MamI 1774 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2605 GATCGTGATC
MroI 2073 TCCGGA T^CCGGA
6100 TCCGGA
NarI 75 GGCGCC GG^CGCC
1087 GGCGCC
PflMI 655 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
988 CCAGATGCTGG
RcaI 1755 TCATGA T^CATGA
5190 TCATGA
SacII 80 CCGCGG CCGC^GG
3956 CCGCGG
SexAI 2913 ACCTGGT A^CC[AT]GGT
4578 ACCTGGT
SfoI 75 GGCGCC GGC^GCC
1087 GGCGCC
SmaI 2104 CCCGGG CCC^GGG
6193 CCCGGG
SphI 4440 GCATGC GCATG^C
4692 GCATGC
Van91I 655 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
988 CCAGATGCTGG
XmaCI 2104 CCCGGG C^CCGGG
6193 CCCGGG
XmaI 2104 CCCGGG C^CCGGG
6193 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 137 enzymes) #
#########################################
AatII AccI AccII AciI AcsI
AcyI AfaI AflIII AluI Alw44I
AlwI AlwNI ApaLI ApoI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvI BcgI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRI
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PshAI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XcmI XhoII