##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AccIII AclI AgeI AscI AseI
AsnI AspI AviII BclI BseAI
BsgI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI Bst1107I BstAPI
BstBI BstZ17I ClaI CpoI EcoRI
EcoRV FbaI FseI FspI MfeI
MroI MunI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SapI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AatII 4372 GACGTC GACGT^C
Acc65I 3798 GGTACC G^GTACC
AflII 2410 CTTAAG C^TTAAG
Alw44I 1745 GTGCAC G^TGCAC
ApaLI 1745 GTGCAC G^TGCAC
Asp718I 3798 GGTACC G^GTACC
AvrII 5455 CCTAGG C^CTAGG
BbvCI 1821 CCTCAGC CCTCAGC(-5/-2)
BciVI 2307 GTATCC GTATCC(6/5)
BfrI 2410 CTTAAG C^TTAAG
BglII 3865 AGATCT A^GATCT
BlnI 5455 CCTAGG C^CTAGG
BsaBI 2489 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 372 TCCGGT [AT]^CCGG[AT]
BsrFI 1771 GCCGGC [GA]^CCGG[CT]
BssSI 4837 CACGAG CACGAG(-5/-1)
BstXI 24 CCACTAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1771 GCCGGC [GA]^CCGG[CT]
DrdI 668 GACATCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2680 CGGCCG C^GGCCG
EclXI 2680 CGGCCG C^GGCCG
Eco52I 2680 CGGCCG C^GGCCG
HpaI 5562 GTTAAC GTT^AAC
KpnI 3798 GGTACC GGTAC^C
KspI 3521 CCGCGG CCGC^GG
MamI 2489 GATACACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1771 GCCGGC GCC^GGC
NdeI 5700 CATATG CA^TATG
NgoMIV 1771 GCCGGC G^CCGGC
NheI 538 GCTAGC G^CTAGC
SacII 3521 CCGCGG CCGC^GG
ScaI 2508 AGTACT AGT^ACT
StuI 4335 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 1554 ACGCGT A^C[GA][CT]GT
2610 ACGCGT
AocI 1381 CCTGAGG CC^T[ACGT]AGG
2498 CCTCAGG
Aor51HI 1453 AGCGCT AGC^GCT
3479 AGCGCT
Asp700I 630 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3263 GAAGACCTTC
BlpI 2296 GCTTAGC GC^T[ACGT]AGC
5440 GCTCAGC
Bpu1102I 2296 GCTTAGC GC^T[ACGT]AGC
5440 GCTCAGC
BsiEI 2680 CGGCCG CG[GA][CT]^CG
3464 CGACCG
BsrBI 2827 CCGCTC CCGCTC(-3/-3)
2025 GAGCGG
BsrDI 97 CATTGC GCAATG(2/0)
5159 CATTGC
BstEII 2763 GGTCACC G^GT[ACGT]ACC
3053 GGTGACC
BstPI 2763 GGTCACC G^GT[ACGT]ACC
3053 GGTGACC
Bsu36I 1381 CCTGAGG CC^T[ACGT]AGG
2498 CCTCAGG
CelII 2296 GCTTAGC GC^T[ACGT]AGC
5440 GCTCAGC
DraI 3248 TTTAAA TTT^AAA
4850 TTTAAA
DraIII 888 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1406 CACTCGGTG
Eco47III 1453 AGCGCT AGC^GCT
3479 AGCGCT
Eco81I 1381 CCTGAGG CC^T[ACGT]AGG
2498 CCTCAGG
EcoO65I 2763 GGTCACC G^GT[ACGT]ACC
3053 GGTGACC
EcoT22I 3593 ATGCAT ATGCA^T
5948 ATGCAT
MluI 1554 ACGCGT A^CGCGT
2610 ACGCGT
NsiI 3593 ATGCAT ATGCA^T
5948 ATGCAT
PshAI 2008 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2753 GACGCGGGTC
PvuII 2994 CAGCTG CAG^CTG
5137 CAGCTG
SalI 1294 GTCGAC G^TCGAC
2236 GTCGAC
SphI 3595 GCATGC GCATG^C
4636 GCATGC
SspI 1222 AATATT AAT^ATT
5979 AATATT
XmnI 630 GAAAGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3263 GAAGACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AhdI AluI AlwI AlwNI
ApaI ApoI AspEI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvI BcgI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrI BssHII BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI