##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccIII AflII AgeI AhdI
AscI Asp700I AspEI AspI AviII
BcgI BfrI BsaWI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BsrGI
BssHII BstEII BstPI ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
EcoO65I EcoRV FseI FspI HindIII
KspI MluI MroI NotI NruI
PacI PaeR7I PflFI PinAI PmeI
PvuI RsrII SacII SalI SbfI
SexAI SfiI SgrAI SnaBI SpeI
Sse8387I SspBI SwaI Tth111I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 7182 GGTACC G^GTACC
AccII 4783 CGCG CG^CG
AclI 7400 AACGTT AA^CGTT
AflIII 694 ACATGT A^C[GA][CT]GT
Alw44I 1945 GTGCAC G^TGCAC
ApaLI 1945 GTGCAC G^TGCAC
Asp718I 7182 GGTACC G^GTACC
AvaI 4189 CCCGGG C^[CT]CG[GA]G
BbrPI 5825 CACGTG CAC^GTG
BciVI 6462 GTATCC GTATCC(6/5)
BglI 2235 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 5301 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 5301 GCTCAGC GC^T[ACGT]AGC
BsaAI 5825 CACGTG [CT]AC^GT[GA]
BsmBI 6374 CGTCTC CGTCTC(1/5)
BsoBI 4189 CCCGGG C^[CT]CG[GA]G
BspLU11I 694 ACATGT A^CATGT
BsrDI 2203 CATTGC GCAATG(2/0)
BssSI 4356 CACGAG CACGAG(-5/-1)
Bst1107I 6701 GTATAC GTA^TAC
BstBI 564 TTCGAA TT^CGAA
BstUI 4783 CGCG CG^CG
BstZ17I 6701 GTATAC GTA^TAC
CelII 5301 GCTCAGC GC^T[ACGT]AGC
DraIII 4977 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 3219 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2076 GAATTC G^AATTC
KpnI 7182 GGTACC GGTAC^C
MfeI 1693 CAATTG C^AATTG
MunI 1693 CAATTG C^AATTG
MvnI 4783 CGCG CG^CG
NaeI 3892 GCCGGC GCC^GGC
NgoMIV 3892 GCCGGC G^CCGGC
NheI 5625 GCTAGC G^CTAGC
NspV 564 TTCGAA TT^CGAA
PmaCI 5825 CACGTG CAC^GTG
PmlI 5825 CACGTG CAC^GTG
PshAI 4557 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 7400 AACGTT AA^CGTT
SfuI 564 TTCGAA TT^CGAA
SmaI 4189 CCCGGG CCC^GGG
XmaCI 4189 CCCGGG C^CCGGG
XmaI 4189 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Aor51HI 4039 AGCGCT AGC^GCT
4177 AGCGCT
AvrII 5847 CCTAGG C^CTAGG
7948 CCTAGG
BamHI 5183 GGATCC G^GATCC
5843 GGATCC
BbeI 1612 GGCGCC GGCGC^C
3895 GGCGCC
BglII 1823 AGATCT A^GATCT
4408 AGATCT
BlnI 5847 CCTAGG C^CTAGG
7948 CCTAGG
BsaBI 1497 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7027 GATGCTCATC
BsaI 2832 GAGACC GGTCTC(1/5)
5867 GAGACC
BspHI 941 TCATGA T^CATGA
6514 TCATGA
BsrBI 3878 CCGCTC CCGCTC(-3/-3)
4415 CCGCTC
BstAPI 5478 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5739 GCAGCAGCTGC
Eco47III 4039 AGCGCT AGC^GCT
4177 AGCGCT
EcoT22I 704 ATGCAT ATGCA^T
1288 ATGCAT
HpaI 640 GTTAAC GTT^AAC
7556 GTTAAC
KasI 1612 GGCGCC G^GCGCC
3895 GGCGCC
MamI 1497 GATGGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7027 GATGCTCATC
NarI 1612 GGCGCC GG^CGCC
3895 GGCGCC
NdeI 1191 CATATG CA^TATG
8048 CATATG
NsiI 704 ATGCAT ATGCA^T
1288 ATGCAT
RcaI 941 TCATGA T^CATGA
6514 TCATGA
ScaI 7220 AGTACT AGT^ACT
7757 AGTACT
SfoI 1612 GGCGCC GGC^GCC
3895 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AseI AsnI AspHI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BclI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PshBI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII