##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

AatII       Acc65I      AccIII      AcyI        AgeI        
Aor51HI     AscI        Asp718I     AspI        AvaI        
BbeI        BbsI        BcgI        BciVI       BlpI        
Bpu1102I    BpuAI       BsaHI       BseAI       BsiEI       
BsiWI       BsoBI       BspDI       BspEI       BssHII      
Bst1107I    BstBI       BstZ17I     CelII       ClaI        
CpoI        EagI        EclXI       Eco47III    Eco52I      
FseI        Hin1I       HincII      HindII      HpaI        
KasI        KpnI        MfeI        MluI        MroI        
MunI        NarI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       PvuI        RsrII       SalI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SwaI        
Tth111I     XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccI          515  GTAGAC               GT^[AC][GT]AC
AccII        2933  CGCG                 CG^CG
AclI         3173  AACGTT               AA^CGTT
AflII         550  CTTAAG               C^TTAAG
AflIII       1324  ACATGT               A^C[GA][CT]GT
Alw44I       6819  GTGCAC               G^TGCAC
ApaI         6876  GGGCCC               GGGCC^C
ApaLI        6819  GTGCAC               G^TGCAC
AvrII        6235  CCTAGG               C^CTAGG
BamHI        4799  GGATCC               G^GATCC
BbrPI        5069  CACGTG               CAC^GTG
BfrI          550  CTTAAG               C^TTAAG
BlnI         6235  CCTAGG               C^CTAGG
BsaAI        5069  CACGTG               [CT]AC^GT[GA]
BsaBI        1660  GATGGCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2393  TCCGGT               [AT]^CCGG[AT]
BspLU11I     1324  ACATGT               A^CATGT
BsrDI        5601  GCAATG               GCAATG(2/0)
BsrFI        5093  GCCGGC               [GA]^CCGG[CT]
BstEII       4678  GGTGACC              G^GT[ACGT]ACC
BstPI        4678  GGTGACC              G^GT[ACGT]ACC
BstUI        2933  CGCG                 CG^CG
Cfr10I       5093  GCCGGC               [GA]^CCGG[CT]
DraIII       6213  CACTTAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I      4678  GGTGACC              G^GT[ACGT]ACC
EcoRI        2699  GAATTC               G^AATTC
KspI         2932  CCGCGG               CCGC^GG
MamI         1660  GATGGCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI         2933  CGCG                 CG^CG
NaeI         5093  GCCGGC               GCC^GGC
NgoMIV       5093  GCCGGC               G^CCGGC
PmaCI        5069  CACGTG               CAC^GTG
PmlI         5069  CACGTG               CAC^GTG
Psp1406I     3173  AACGTT               AA^CGTT
SacI         1542  GAGCTC               GAGCT^C
SacII        2932  CCGCGG               CCGC^GG
SapI          413  GAAGAGC              GCTCTTC(1/4)
ScaI         3507  AGTACT               AGT^ACT
SphI         3776  GCATGC               GCATG^C


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AhdI          772  GACAAAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4656  GACTCCACGTC         
AocI         2555  CCTCAGG              CC^T[ACGT]AGG
             6612  CCTAAGG             
AseI         2089  ATTAAT               AT^TAAT
             2275  ATTAAT              
AsnI         2089  ATTAAT               AT^TAAT
             2275  ATTAAT              
AspEI         772  GACAAAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4656  GACTCCACGTC         
AviII        1808  TGCGCA               TGC^GCA
             4277  TGCGCA              
BanI         3786  GGCACC               G^G[CT][GA]CC
             4882  GGTGCC              
BclI         1396  TGATCA               T^GATCA
             7048  TGATCA              
BcnI         6565  CCGGG                CC^[GC]GG
             6907  CCGGG               
BglI          281  GCCAAACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             3033  GCCTGCTCGGC         
BsmBI        2499  CGTCTC               CGTCTC(1/5)
             6718  CGTCTC              
Bsp1407I     1321  TGTACA               T^GTACA
             1959  TGTACA              
BsrGI        1321  TGTACA               T^GTACA
             1959  TGTACA              
Bsu36I       2555  CCTCAGG              CC^T[ACGT]AGG
             6612  CCTAAGG             
Eam1105I      772  GACAAAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             4656  GACTCCACGTC         
Eco81I       2555  CCTCAGG              CC^T[ACGT]AGG
             6612  CCTAAGG             
EcoRV        1979  GATATC               GAT^ATC
             7280  GATATC              
EcoT22I      3458  ATGCAT               ATGCA^T
             3559  ATGCAT              
FauI         2613  CCCGC                CCCGC(4/6)
             2934  GCGGG               
FbaI         1396  TGATCA               T^GATCA
             7048  TGATCA              
FspI         1808  TGCGCA               TGC^GCA
             4277  TGCGCA              
HaeII        4307  GGCGCT               [GA]GCGC^[CT]
             6643  GGCGCT              
HgaI         6536  GACGC                GACGC(5/10)
              211  GCGTC               
HindIII      2527  AAGCTT               A^AGCTT
             5781  AAGCTT              
NciI         6565  CCGGG                CC^[GC]GG
             6907  CCGGG               
NdeI          819  CATATG               CA^TATG
             5035  CATATG              
NsiI         3458  ATGCAT               ATGCA^T
             3559  ATGCAT              
PshBI        2089  ATTAAT               AT^TAAT
             2275  ATTAAT              
PstI         4597  CTGCAG               CTGCA^G
             6580  CTGCAG              
SspBI        1321  TGTACA               T^GTACA
             1959  TGTACA              
TaqI          390  TCGA                 T^CGA
             2035  TCGA                
TthHB8I       390  TCGA                 T^CGA
             2035  TCGA                
XbaI         1889  TCTAGA               T^CTAGA
             6631  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AspHI       AvaII       
BalI        BanII       BbvCI       BbvI        BfaI        
BglII       BmyI        BpmI        BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsmI        Bsp1286I    BspHI       
BspMI       BsrBI       BsrI        BssKI       BssSI       
BstAPI      BstF5I      BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       DrdI        DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NcoI        NdeII       NheI        NlaIII      
NlaIV       NspI        PflMI       PleI        PpuMI       
PvuII       RcaI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspI        
StuI        StyI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Van91I      XhoII       
XmnI