##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
Aor51HI AscI Asp718I AspI AvaI
BbeI BbsI BcgI BciVI BlpI
Bpu1102I BpuAI BsaHI BseAI BsiEI
BsiWI BsoBI BspDI BspEI BssHII
Bst1107I BstBI BstZ17I CelII ClaI
CpoI EagI EclXI Eco47III Eco52I
FseI Hin1I HincII HindII HpaI
KasI KpnI MfeI MluI MroI
MunI NarI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PvuI RsrII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
Tth111I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 515 GTAGAC GT^[AC][GT]AC
AccII 2933 CGCG CG^CG
AclI 3173 AACGTT AA^CGTT
AflII 550 CTTAAG C^TTAAG
AflIII 1324 ACATGT A^C[GA][CT]GT
Alw44I 6819 GTGCAC G^TGCAC
ApaI 6876 GGGCCC GGGCC^C
ApaLI 6819 GTGCAC G^TGCAC
AvrII 6235 CCTAGG C^CTAGG
BamHI 4799 GGATCC G^GATCC
BbrPI 5069 CACGTG CAC^GTG
BfrI 550 CTTAAG C^TTAAG
BlnI 6235 CCTAGG C^CTAGG
BsaAI 5069 CACGTG [CT]AC^GT[GA]
BsaBI 1660 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2393 TCCGGT [AT]^CCGG[AT]
BspLU11I 1324 ACATGT A^CATGT
BsrDI 5601 GCAATG GCAATG(2/0)
BsrFI 5093 GCCGGC [GA]^CCGG[CT]
BstEII 4678 GGTGACC G^GT[ACGT]ACC
BstPI 4678 GGTGACC G^GT[ACGT]ACC
BstUI 2933 CGCG CG^CG
Cfr10I 5093 GCCGGC [GA]^CCGG[CT]
DraIII 6213 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 4678 GGTGACC G^GT[ACGT]ACC
EcoRI 2699 GAATTC G^AATTC
KspI 2932 CCGCGG CCGC^GG
MamI 1660 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2933 CGCG CG^CG
NaeI 5093 GCCGGC GCC^GGC
NgoMIV 5093 GCCGGC G^CCGGC
PmaCI 5069 CACGTG CAC^GTG
PmlI 5069 CACGTG CAC^GTG
Psp1406I 3173 AACGTT AA^CGTT
SacI 1542 GAGCTC GAGCT^C
SacII 2932 CCGCGG CCGC^GG
SapI 413 GAAGAGC GCTCTTC(1/4)
ScaI 3507 AGTACT AGT^ACT
SphI 3776 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AhdI 772 GACAAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4656 GACTCCACGTC
AocI 2555 CCTCAGG CC^T[ACGT]AGG
6612 CCTAAGG
AseI 2089 ATTAAT AT^TAAT
2275 ATTAAT
AsnI 2089 ATTAAT AT^TAAT
2275 ATTAAT
AspEI 772 GACAAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4656 GACTCCACGTC
AviII 1808 TGCGCA TGC^GCA
4277 TGCGCA
BanI 3786 GGCACC G^G[CT][GA]CC
4882 GGTGCC
BclI 1396 TGATCA T^GATCA
7048 TGATCA
BcnI 6565 CCGGG CC^[GC]GG
6907 CCGGG
BglI 281 GCCAAACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3033 GCCTGCTCGGC
BsmBI 2499 CGTCTC CGTCTC(1/5)
6718 CGTCTC
Bsp1407I 1321 TGTACA T^GTACA
1959 TGTACA
BsrGI 1321 TGTACA T^GTACA
1959 TGTACA
Bsu36I 2555 CCTCAGG CC^T[ACGT]AGG
6612 CCTAAGG
Eam1105I 772 GACAAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4656 GACTCCACGTC
Eco81I 2555 CCTCAGG CC^T[ACGT]AGG
6612 CCTAAGG
EcoRV 1979 GATATC GAT^ATC
7280 GATATC
EcoT22I 3458 ATGCAT ATGCA^T
3559 ATGCAT
FauI 2613 CCCGC CCCGC(4/6)
2934 GCGGG
FbaI 1396 TGATCA T^GATCA
7048 TGATCA
FspI 1808 TGCGCA TGC^GCA
4277 TGCGCA
HaeII 4307 GGCGCT [GA]GCGC^[CT]
6643 GGCGCT
HgaI 6536 GACGC GACGC(5/10)
211 GCGTC
HindIII 2527 AAGCTT A^AGCTT
5781 AAGCTT
NciI 6565 CCGGG CC^[GC]GG
6907 CCGGG
NdeI 819 CATATG CA^TATG
5035 CATATG
NsiI 3458 ATGCAT ATGCA^T
3559 ATGCAT
PshBI 2089 ATTAAT AT^TAAT
2275 ATTAAT
PstI 4597 CTGCAG CTGCA^G
6580 CTGCAG
SspBI 1321 TGTACA T^GTACA
1959 TGTACA
TaqI 390 TCGA T^CGA
2035 TCGA
TthHB8I 390 TCGA T^CGA
2035 TCGA
XbaI 1889 TCTAGA T^CTAGA
6631 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BalI BanII BbvCI BbvI BfaI
BglII BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BspMI BsrBI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DrdI DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NheI NlaIII
NlaIV NspI PflMI PleI PpuMI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII
XmnI