##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
Acc65I AccIII AgeI AhdI AscI
Asp718I AspEI AspI AviII BcgI
BglII BseAI BsiWI BspDI BspEI
BspHI BssSI ClaI CpoI Eam1105I
EcoRV FspI KpnI MluI MroI
NcoI NheI NotI NruI PacI
PaeR7I PflFI PinAI PmeI PvuI
RcaI RsrII SapI SbfI ScaI
SgrAI SnaBI SpeI Sse8387I SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 983 GACGTC GACGT^C
AclI 833 AACGTT AA^CGTT
AflII 2160 CTTAAG C^TTAAG
Alw44I 4957 GTGCAC G^TGCAC
Aor51HI 5990 AGCGCT AGC^GCT
ApaLI 4957 GTGCAC G^TGCAC
BbvCI 4268 CCTCAGC CCTCAGC(-5/-2)
BclI 4994 TGATCA T^GATCA
BfrI 2160 CTTAAG C^TTAAG
BsmI 6396 GAATGC GAATGC(1/-1)
BsrBI 250 CCGCTC CCGCTC(-3/-3)
BssHII 432 GCGCGC G^CGCGC
Bst1107I 6600 GTATAC GTA^TAC
BstEII 235 GGTCACC G^GT[ACGT]ACC
BstPI 235 GGTCACC G^GT[ACGT]ACC
BstXI 3761 CCAATAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 6600 GTATAC GTA^TAC
Eco47III 5990 AGCGCT AGC^GCT
EcoO65I 235 GGTCACC G^GT[ACGT]ACC
EcoT22I 5838 ATGCAT ATGCA^T
FbaI 4994 TGATCA T^GATCA
FseI 111 GGCCGGCC GGCCGG^CC
HgaI 5 GACGC GACGC(5/10)
HindIII 5170 AAGCTT A^AGCTT
HpaI 2374 GTTAAC GTT^AAC
KspI 117 CCGCGG CCGC^GG
MfeI 2236 CAATTG C^AATTG
MunI 2236 CAATTG C^AATTG
NdeI 2974 CATATG CA^TATG
NsiI 5838 ATGCAT ATGCA^T
Psp1406I 833 AACGTT AA^CGTT
SacI 1649 GAGCTC GAGCT^C
SacII 117 CCGCGG CCGC^GG
SalI 6509 GTCGAC G^TCGAC
SfiI 2713 GGCCTTTTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1243 GCATGC GCATG^C
XbaI 5878 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AocI 2465 CCTTAGG CC^T[ACGT]AGG
3289 CCTGAGG
AvrII 4003 CCTAGG C^CTAGG
6019 CCTAGG
BamHI 2482 GGATCC G^GATCC
4007 GGATCC
BbrPI 1386 CACGTG CAC^GTG
3613 CACGTG
BlnI 4003 CCTAGG C^CTAGG
6019 CCTAGG
BsmBI 43 CGTCTC CGTCTC(1/5)
3 GAGACG
Bsu36I 2465 CCTTAGG CC^T[ACGT]AGG
3289 CCTGAGG
DraIII 4332 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
6496 CACAAAGTG
DsaI 117 CCGCGG C^C[GA][CT]GG
408 CCGTGG
Eco81I 2465 CCTTAGG CC^T[ACGT]AGG
3289 CCTGAGG
PmaCI 1386 CACGTG CAC^GTG
3613 CACGTG
PmlI 1386 CACGTG CAC^GTG
3613 CACGTG
PshAI 2538 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5578 GACCCCAGTC
PvuII 681 CAGCTG CAG^CTG
3720 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 160 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApaI ApoI AseI AsnI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvI BciVI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspLU11I BspMI BsrDI BsrFI BsrGI
BsrI BssKI BstAPI BstBI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DrdI EaeI EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NspI
NspV PflMI PleI PpuMI PshBI
PstI RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SfoI SfuI
SmaI SmlI SspBI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI XmnI