##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AccIII AclI AflII AscI
AseI AsnI Asp718I AviII BbeI
BciVI BclI BfrI BsaBI BseAI
BsiWI BspDI BspEI BssHII Bst1107I
BstEII BstPI BstZ17I ClaI CpoI
EagI EclXI Eco52I EcoO65I EcoT22I
FbaI FseI FspI HpaI KasI
KpnI MamI MroI NarI NcoI
NdeI NotI NruI NsiI PacI
PmeI PshBI Psp1406I PvuI RsrII
SalI SexAI SfiI SfoI SnaBI
SpeI SwaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AgeI 5364 ACCGGT A^CCGGT
ApaI 1343 GGGCCC GGGCC^C
Asp700I 6158 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1353 GGATCC G^GATCC
BcgI 1889 GCAGGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsiEI 803 CGACCG CG[GA][CT]^CG
Bsp1407I 2726 TGTACA T^GTACA
BsrGI 2726 TGTACA T^GTACA
BstBI 2164 TTCGAA TT^CGAA
EcoRI 335 GAATTC G^AATTC
EcoRV 7244 GATATC GAT^ATC
KspI 1193 CCGCGG CCGC^GG
MfeI 5134 CAATTG C^AATTG
MunI 5134 CAATTG C^AATTG
NaeI 2120 GCCGGC GCC^GGC
NgoMIV 2120 GCCGGC G^CCGGC
NheI 7230 GCTAGC G^CTAGC
NspV 2164 TTCGAA TT^CGAA
PinAI 5364 ACCGGT A^CCGGT
PshAI 810 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 756 GAGCTC GAGCT^C
SacII 1193 CCGCGG CCGC^GG
SapI 6158 GAAGAGC GCTCTTC(1/4)
SbfI 2283 CCTGCAGG CCTGCA^GG
ScaI 2036 AGTACT AGT^ACT
SfuI 2164 TTCGAA TT^CGAA
SgrAI 5363 CACCGGTG C[GA]^CCGG[CT]G
Sse8387I 2283 CCTGCAGG CCTGCA^GG
SspBI 2726 TGTACA T^GTACA
SspI 1249 AATATT AAT^ATT
XmnI 6158 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 2951 GTAGAC GT^[AC][GT]AC
3944 GTCTAC
BglI 3802 GCCAGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5140 GCCTGGCAGGC
BglII 2396 AGATCT A^GATCT
7313 AGATCT
BsaI 6740 GAGACC GGTCTC(1/5)
2224 GGTCTC
BsmI 2167 GAATGC GAATGC(1/-1)
4748 GAATGC
BspHI 5590 TCATGA T^CATGA
6353 TCATGA
BspLU11I 2772 ACATGT A^CATGT
4309 ACATGT
BsrBI 565 CCGCTC CCGCTC(-3/-3)
3690 CCGCTC
BsrDI 1689 CATTGC GCAATG(2/0)
3445 GCAATG
DrdI 1714 GACTGTGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5280 GACTGGAGGGTC
HindIII 1057 AAGCTT A^AGCTT
4316 AAGCTT
MluI 2177 ACGCGT A^CGCGT
3573 ACGCGT
PaeR7I 713 CTCGAG C^TCGAG
1901 CTCGAG
RcaI 5590 TCATGA T^CATGA
6353 TCATGA
XbaI 7042 TCTAGA T^CTAGA
7290 TCTAGA
XhoI 713 CTCGAG C^TCGAG
1901 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 154 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AflIII AhdI AluI Alw44I
AlwI AlwNI AocI Aor51HI ApaLI
ApoI AspEI AspHI AspI AvaI
AvaII AvrII BalI BanI BanII
BbrPI BbsI BbvCI BbvI BcnI
BfaI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
Eam1105I EarI Eco47III Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SphI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmaCI XmaI