##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AccI AccIII AgeI AhdI
AscI AspEI AviII BalI BseAI
BsiEI BspDI BspEI BssHII Bst1107I
BstEII BstPI BstZ17I ClaI CpoI
EaeI EagI Eam1105I EclXI Eco52I
EcoO65I FseI FspI KspI MluI
MluNI MroI MscI NheI NotI
NruI PacI PaeR7I PinAI PmeI
PshAI PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SgrAI
SmaI SpeI Sse8387I SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 6966 GGTACC G^GTACC
AclI 5078 AACGTT AA^CGTT
Asp718I 6966 GGTACC G^GTACC
AspI 813 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1008 CCCGAG C^[CT]CG[GA]G
AvrII 4012 CCTAGG C^CTAGG
BamHI 3491 GGATCC G^GATCC
BbeI 2160 GGCGCC GGCGC^C
BcgI 3176 CGAGCTCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 4012 CCTAGG C^CTAGG
BsaI 1167 GAGACC GGTCTC(1/5)
BsaWI 852 ACCGGA [AT]^CCGG[AT]
BseRI 931 GAGGAG GAGGAG(10/8)
BsiWI 1801 CGTACG C^GTACG
BsoBI 1008 CCCGAG C^[CT]CG[GA]G
BsrBI 1208 GAGCGG CCGCTC(-3/-3)
BssSI 374 CACGAG CACGAG(-5/-1)
BstBI 3810 TTCGAA TT^CGAA
DrdI 6331 GACATTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 5649 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 3239 GAATTC G^AATTC
EcoRV 6197 GATATC GAT^ATC
EcoT22I 7164 ATGCAT ATGCA^T
KasI 2160 GGCGCC G^GCGCC
KpnI 6966 GGTACC GGTAC^C
MfeI 5669 CAATTG C^AATTG
MunI 5669 CAATTG C^AATTG
NarI 2160 GGCGCC GG^CGCC
NsiI 7164 ATGCAT ATGCA^T
NspV 3810 TTCGAA TT^CGAA
PflFI 813 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 5693 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 5078 AACGTT AA^CGTT
PvuII 1442 CAGCTG CAG^CTG
SacI 3177 GAGCTC GAGCT^C
ScaI 2739 AGTACT AGT^ACT
SfoI 2160 GGCGCC GGC^GCC
SfuI 3810 TTCGAA TT^CGAA
SnaBI 5434 TACGTA TAC^GTA
StuI 5657 AGGCCT AGG^CCT
Tth111I 813 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 5693 CCACTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 5855 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 2160 GGCGCC G[GA]^CG[CT]C
3478 GGCGTC
AflII 2726 CTTAAG C^TTAAG
4696 CTTAAG
AocI 1269 CCTGAGG CC^T[ACGT]AGG
6063 CCTGAGG
Aor51HI 1246 AGCGCT AGC^GCT
1822 AGCGCT
BbvCI 1920 GCTGAGG CCTCAGC(-5/-2)
3767 GCTGAGG
BfrI 2726 CTTAAG C^TTAAG
4696 CTTAAG
BglI 967 GCCCTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1789 GCCAGGATGGC
BlpI 1818 GCTGAGC GC^T[ACGT]AGC
5036 GCTGAGC
Bpu1102I 1818 GCTGAGC GC^T[ACGT]AGC
5036 GCTGAGC
BsaBI 621 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5719 GATAGGCATC
BsaHI 2160 GGCGCC G[GA]^CG[CT]C
3478 GGCGTC
BsmBI 3480 CGTCTC CGTCTC(1/5)
3924 GAGACG
BstAPI 1230 GCAGGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2290 GCAGTGACTGC
Bsu36I 1269 CCTGAGG CC^T[ACGT]AGG
6063 CCTGAGG
CelII 1818 GCTGAGC GC^T[ACGT]AGC
5036 GCTGAGC
Eco47III 1246 AGCGCT AGC^GCT
1822 AGCGCT
Eco81I 1269 CCTGAGG CC^T[ACGT]AGG
6063 CCTGAGG
Hin1I 2160 GGCGCC G[GA]^CG[CT]C
3478 GGCGTC
HindIII 1423 AAGCTT A^AGCTT
5914 AAGCTT
MamI 621 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5719 GATAGGCATC
NaeI 3143 GCCGGC GCC^GGC
3362 GCCGGC
NcoI 545 CCATGG C^CATGG
6427 CCATGG
NgoMIV 3143 GCCGGC G^CCGGC
3362 GCCGGC
SphI 1215 GCATGC GCATG^C
2869 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI AseI AsnI Asp700I
AspHI AvaII BanI BanII BbrPI
BbsI BbvI BciVI BclI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BspHI BspLU11I BspMI BsrDI
BsrFI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EarI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PleI PmaCI PmlI PpuMI
PshBI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI