##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AccIII AgeI AscI AseI AsnI
AspI AvrII BbeI BbrPI BcgI
BclI BglI BlnI BsaAI BseAI
BsiEI BsiWI BspDI BspEI BssHII
BssSI ClaI CpoI EagI EclXI
Eco52I FauI FbaI FseI KasI
KspI MluI MroI NarI NheI
NotI NruI PacI PflFI PinAI
PmaCI PmeI PmlI PshBI PvuI
RsrII SacII SfiI SfoI SgrAI
SmaI SnaBI SwaI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 7287 GACGTC GACGT^C
Acc65I 1440 GGTACC G^GTACC
AccII 2758 CGCG CG^CG
AclI 4219 AACGTT AA^CGTT
AcyI 7287 GACGTC G[GA]^CG[CT]C
AhdI 2768 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 26 GTGCAC G^TGCAC
Aor51HI 1482 AGCGCT AGC^GCT
ApaLI 26 GTGCAC G^TGCAC
Asp718I 1440 GGTACC G^GTACC
AspEI 2768 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2577 CTCGAG C^[CT]CG[GA]G
BamHI 3906 GGATCC G^GATCC
BsaHI 7287 GACGTC G[GA]^CG[CT]C
BsaI 7866 GGTCTC GGTCTC(1/5)
BsaWI 3690 TCCGGT [AT]^CCGG[AT]
BsmBI 5178 CGTCTC CGTCTC(1/5)
BsoBI 2577 CTCGAG C^[CT]CG[GA]G
BsrBI 1963 GAGCGG CCGCTC(-3/-3)
BsrFI 3733 GCCGGC [GA]^CCGG[CT]
Bst1107I 6914 GTATAC GTA^TAC
BstAPI 2920 GCAGTATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1475 TTCGAA TT^CGAA
BstUI 2758 CGCG CG^CG
BstZ17I 6914 GTATAC GTA^TAC
Cfr10I 3733 GCCGGC [GA]^CCGG[CT]
DraIII 4569 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 671 GACATGAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2768 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1482 AGCGCT AGC^GCT
EcoRI 1549 GAATTC G^AATTC
EcoRV 305 GATATC GAT^ATC
Hin1I 7287 GACGTC G[GA]^CG[CT]C
HpaI 4245 GTTAAC GTT^AAC
KpnI 1440 GGTACC GGTAC^C
MfeI 562 CAATTG C^AATTG
MunI 562 CAATTG C^AATTG
MvnI 2758 CGCG CG^CG
NaeI 3733 GCCGGC GCC^GGC
NcoI 345 CCATGG C^CATGG
NgoMIV 3733 GCCGGC G^CCGGC
NspV 1475 TTCGAA TT^CGAA
PaeR7I 2577 CTCGAG C^TCGAG
PshAI 6929 GACTTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4219 AACGTT AA^CGTT
SbfI 6832 CCTGCAGG CCTGCA^GG
ScaI 6261 AGTACT AGT^ACT
SfuI 1475 TTCGAA TT^CGAA
SpeI 2954 ACTAGT A^CTAGT
Sse8387I 6832 CCTGCAGG CCTGCA^GG
XbaI 3154 TCTAGA T^CTAGA
XhoI 2577 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflII 5087 CTTAAG C^TTAAG
7459 CTTAAG
ApaI 1233 GGGCCC GGGCC^C
1768 GGGCCC
Asp700I 5387 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7574 GAAGTGCTTC
AviII 5844 TGCGCA TGC^GCA
7969 TGCGCA
BalI 612 TGGCCA TGG^CCA
4099 TGGCCA
BciVI 6241 GGATAC GTATCC(6/5)
6774 GGATAC
BcnI 855 CCCGG CC^[GC]GG
2540 CCCGG
BfrI 5087 CTTAAG C^TTAAG
7459 CTTAAG
BglII 230 AGATCT A^GATCT
6489 AGATCT
BsmI 1708 GAATGC GAATGC(1/-1)
4213 GAATGC
BstEII 139 GGTAACC G^GT[ACGT]ACC
5485 GGTAACC
BstPI 139 GGTAACC G^GT[ACGT]ACC
5485 GGTAACC
DsaI 345 CCATGG C^C[GA][CT]GG
3027 CCGTGG
EcoO65I 139 GGTAACC G^GT[ACGT]ACC
5485 GGTAACC
FspI 5844 TGCGCA TGC^GCA
7969 TGCGCA
HgaI 5177 GCGTC GACGC(5/10)
7516 GCGTC
MluNI 612 TGGCCA TGG^CCA
4099 TGGCCA
MscI 612 TGGCCA TGG^CCA
4099 TGGCCA
NciI 855 CCCGG CC^[GC]GG
2540 CCCGG
PpuMI 3795 AGGACCC [GA]G^G[AT]CC[CT]
7278 GGGTCCC
SalI 918 GTCGAC G^TCGAC
2487 GTCGAC
SexAI 2058 ACCAGGT A^CC[AT]GGT
6178 ACCTGGT
XmnI 5387 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
7574 GAAGTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BanI BanII BbsI
BbvCI BbvI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaBI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspHI
BspLU11I BspMI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PstI
PvuII RcaI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI SspBI SspI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII