##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BbeI BcgI BclI
BlnI BsaHI BseAI BsiWI BspDI
BspEI BspHI BsrBI BssHII BstXI
ClaI CpoI Eam1105I Eco47III FbaI
FseI FspI Hin1I KasI KpnI
KspI MfeI MluI MroI MunI
NaeI NarI NgoMIV NheI NotI
NruI PacI PinAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I XcmI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AflII 3758 CTTAAG C^TTAAG
Alw44I 7699 GTGCAC G^TGCAC
ApaLI 7699 GTGCAC G^TGCAC
AspI 6632 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 5198 CACGTG CAC^GTG
BcnI 2048 CCCGG CC^[GC]GG
BfrI 3758 CTTAAG C^TTAAG
BsiEI 2951 CGGCCG CG[GA][CT]^CG
BsrFI 582 GCCGGT [GA]^CCGG[CT]
BstBI 6756 TTCGAA TT^CGAA
Cfr10I 582 GCCGGT [GA]^CCGG[CT]
DraIII 3565 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 777 GACCATGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2951 CGGCCG C^GGCCG
EclXI 2951 CGGCCG C^GGCCG
Eco52I 2951 CGGCCG C^GGCCG
EcoNI 5782 CCTATGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 470 AGCGCC [GA]GCGC^[CT]
HgaI 4994 GACGC GACGC(5/10)
HpaI 4298 GTTAAC GTT^AAC
NciI 2048 CCCGG CC^[GC]GG
NspV 6756 TTCGAA TT^CGAA
PflFI 6632 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3564 CCACAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 5198 CACGTG CAC^GTG
PmeI 4803 GTTTAAAC GTTT^AAAC
PmlI 5198 CACGTG CAC^GTG
PshAI 2482 GACTAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfuI 6756 TTCGAA TT^CGAA
Tth111I 6632 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3564 CCACAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 2424 CGCG CG^CG
4996 CGCG
AocI 1508 CCTCAGG CC^T[ACGT]AGG
3920 CCTCAGG
BbvCI 2159 CCTCAGC CCTCAGC(-5/-2)
4459 GCTGAGG
BglI 1207 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6376 GCCCTAGAGGC
BlpI 1321 GCTCAGC GC^T[ACGT]AGC
1354 GCTCAGC
Bpu1102I 1321 GCTCAGC GC^T[ACGT]AGC
1354 GCTCAGC
BsaBI 6842 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7469 GATACCAATC
BsaI 1998 GAGACC GGTCTC(1/5)
2296 GAGACC
BsaWI 456 ACCGGA [AT]^CCGG[AT]
5061 ACCGGA
BsmI 1451 GAATGC GAATGC(1/-1)
7638 GAATGC
BspMI 2311 GCAGGT ACCTGC(4/8)
6654 GCAGGT
BssSI 4943 CACGAG CACGAG(-5/-1)
967 CTCGTG
Bst1107I 3191 GTATAC GTA^TAC
6115 GTATAC
BstUI 2424 CGCG CG^CG
4996 CGCG
BstZ17I 3191 GTATAC GTA^TAC
6115 GTATAC
Bsu36I 1508 CCTCAGG CC^T[ACGT]AGG
3920 CCTCAGG
CelII 1321 GCTCAGC GC^T[ACGT]AGC
1354 GCTCAGC
Eco81I 1508 CCTCAGG CC^T[ACGT]AGG
3920 CCTCAGG
EcoRI 297 GAATTC G^AATTC
5958 GAATTC
EcoT22I 6459 ATGCAT ATGCA^T
7676 ATGCAT
HincII 4298 GTTAAC GT[CT]^[GA]AC
5987 GTTGAC
HindII 4298 GTTAAC GT[CT]^[GA]AC
5987 GTTGAC
MamI 6842 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
7469 GATACCAATC
MvnI 2424 CGCG CG^CG
4996 CGCG
NcoI 2282 CCATGG C^CATGG
2476 CCATGG
NsiI 6459 ATGCAT ATGCA^T
7676 ATGCAT
PaeR7I 1386 CTCGAG C^TCGAG
4365 CTCGAG
PstI 202 CTGCAG CTGCA^G
2309 CTGCAG
SapI 931 GAAGAGC GCTCTTC(1/4)
6780 GAAGAGC
SpeI 4583 ACTAGT A^CTAGT
7502 ACTAGT
XbaI 2451 TCTAGA T^CTAGA
3362 TCTAGA
XhoI 1386 CTCGAG C^TCGAG
4365 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvI BciVI BfaI
BglII BmyI BpmI BpuAI BsaAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspLU11I BsrDI BsrGI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoO65I
EcoRII EcoRV EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeI NdeII NlaIII NlaIV
NspI PleI PpuMI PvuII RsaI
SacI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspBI SspI
StuI StyI SwaI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI